Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: DAVID software analyzed the possible biological processes of the significantly affected genes in treated HL60 cells. Biological activation based on extracellular signals was highly affected after TQ treatment. The area of each category represents the percentages of involved genes to each other ( p < 0.05).

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Software, Activation Assay

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: DAVID software analyzed the possible active locations of the significantly affected genes in the cellular components of TQ-treated HL60 cells. The majority of genes were activated in the plasma membrane, which suggests that TQ affects signaling activation in the plasma membrane. The area of each category represents the percentages of involved genes to each other ( p < 0.05).

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Software, Clinical Proteomics, Membrane, Activation Assay

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: RT−qPCR results of JAK2 , STAT3 , STAT5a , and STAT5b expression in HL60 cells. The relative normalization of RT−qPCR showed that TQ significantly downregulated the expression of targeted genes in treated cells. STAT3 and STAT5a were downregulated in treated cells approximately 5−fold lower compared with untreated cells. Data are presented as the mean ± SEM. (*** p < 0.001).

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Quantitative RT-PCR, Expressing

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: RT−qPCR results of PI3K , AKT, and mTOR expression in HL60 cells. The relative normalization of RT−qPCR showed that TQ significantly downregulated the expression of the targeted genes in treated cells. PI3K , AKT, and mTOR were downregulated in treated cells approximately 2-fold lower than in untreated cells. Data are presented as the mean ± SEM (*** p < 0.001).

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Quantitative RT-PCR, Expressing

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: The c− Myc expression level was determined using a RT−qPCR assay in HL60 cells. RT−qPCR revealed that TQ significantly downregulated c − Myc gene expression by 4−fold in treated cells, and the untreated cells were used as a negative control group. Data are presented as the mean ± SEM (*** p < 0.001).

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Expressing, Quantitative RT-PCR, Gene Expression, Negative Control

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: Effects of TQ on the expression of JAK/STAT proteins in HL60 cells. ( A ) JAK2, STAT3, p-STAT3, STAT5, and p-STAT5 protein expression in TQ-treated cells compared with untreated cells or negative control. Data are presented as the mean ± SEM ( *** p < 0.001). ( B ) Images of JAK2, STAT3, p-STAT3, STAT5, and p-STAT5 protein expression from capillary Western blotting in treated and untreated HL60 cells.

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Expressing, Negative Control, Western Blot

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: Effects of TQ on the expression of PI3K/AKT proteins in HL60 cells. ( A ) PI3K, p-PI3K, AKT, p-AKT, and PTEN protein expression in TQ-treated cells compared with untreated cells or negative control. Data are presented as the mean ± SEM (*** p < 0.001; ** p < 0.01). ( B ) Image of PI3K, p-PI3K, AKT, p-AKT, and PTEN protein expression from capillary Western blotting in treated and untreated HL60 cells.

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Expressing, Negative Control, Western Blot

Journal: Pharmaceuticals

Article Title: Gene Expression Profiling and Protein Analysis Reveal Suppression of the C-Myc Oncogene and Inhibition JAK/STAT and PI3K/AKT/mTOR Signaling by Thymoquinone in Acute Myeloid Leukemia Cells

doi: 10.3390/ph15030307

Figure Lengend Snippet: Effects of TQ on the expression of c-Myc protein in HL60 cells. ( A ) c-Myc protein expression in TQ-treated cells compared with untreated cells or negative control. Data are presented as the mean ± SEM (*** p < 0.001). ( B ) Image of c-Myc protein expression from capillary Western blotting in treated and untreated HL60 cells.

Article Snippet: The HL60 cell line was obtained from (Elabscience Biotech Co., Ltd., Wuhan, China) and cultured in a complete medium containing Roswell Park Memorial Institute (RPMI) 1640 Medium (Nacalai Tesque, Kyoto, Japan), fetal bovine serum (FBS) 10% (Tico Europe, Holland), and 1% penicillin/streptomycin (Nacalai Tesque, Kyoto, Japan) in T-25 culture flasks at 37 °C with 5% CO 2 in a humid incubator [ ].

Techniques: Expressing, Negative Control, Western Blot

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: E. coli O175:H7 strains used for antibacterial experiments.

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Isolation

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: Antibacterial activity of synthesized AgNPs at 1000 μg/mL concentrations in the water against E. coli O157:H7 strains ( A – I ).

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Activity Assay, Synthesized

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: Antibacterial efficacy of probiotic-mediated synthesized AgNPs against E. coli O157:H7 strains.

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Synthesized, Inhibition

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: Antibacterial activity of some commercial antibiotics against nine E. coli O157:H7 strains ( A – I ). Abbreviations: P (penicillin G, 10 μg/disc), E (erythromycin, 15 μg/disc), and VA (vancomycin, 30 μg/disc).

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Activity Assay

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: Antibacterial efficacy of some commercial antibiotics against E. coli O157:H7 strains—no inhibition zone.

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Inhibition

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: Growth curves of nine E. coli O157:H7 strains ( A – I ) cultured in LB broth with various concentrations of synthesized AgNPs to determine MIC. Control (●); 200 μg/mL (◦); 100 μg/mL (▼) 50 μg/mL (△); 25 μg/mL (§); 12.5 μg/mL (□); 6.25 μg/mL (w); 3.12 μg/mL (◊).

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Cell Culture, Synthesized, Control

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: MBC of probiotic-mediated synthesized AgNPs against E. coli O157:H7 strains ( A – I ).

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Synthesized

Journal: Polymers

Article Title: Probiotic-Mediated Biosynthesis of Silver Nanoparticles and Their Antibacterial Applications against Pathogenic Strains of Escherichia coli O157:H7

doi: 10.3390/polym14091834

Figure Lengend Snippet: FE-SEM images of normal E. coli O157:H7 (ATCC 35150) ( A , B ) and 1 × MBC probiotic-mediated synthesized AgNPs treated E. coli O157:H7 (ATCC 35150) ( C , D ).

Article Snippet: Minimum bactericidal concentrations (MBCs) were 25 μg/mL for E. coli O157:H7 ATCC 35150, E. coli O157:H7 ATCC 43895, E. coli O157:H7 ATCC 43890, E. coli O157:H7 ATCC 43889, and E. coli ATCC 25922; and 50 μg/mL for E. coli O157:H7 2257, E. coli O157: NM 3204-92, E. coli O157:H7 8624 and E. coli O157:H7 ATCC 43894.

Techniques: Synthesized