Journal: European Journal of Medical Research
Article Title: Deciphering stromal cell interactions in osteosarcoma highlights CDKN2A and MMP14 as novel diagnostic and therapeutic biomarkers
doi: 10.1186/s40001-026-03902-2
Figure Lengend Snippet: Regulatory role of biomarker MMP14 in the malignant phenotype of Osteosarcoma cell lines. A Expression of MMP14 and CDKN2A in cell lines U-2OS and SJSA-1 compared to hFOB1.19. B Construction of MMP14-silenced U-2OS and SJSA-1 cell lines. C CCK-8 assay measuring cell proliferation in U-2OS/si-MMP14 and SJSA-1/si-MMP14 cells. D Transwell assay assessing relative invasion levels in U-2OS/si-MMP14 and SJSA-1/si-MMP14 cells. E Scratch healing assay for relative migration levels in U-2OS/si-MMP14 and SJSA-1/si-MMP14. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Article Snippet: hFOB1.19, U-2 OS, and SJSA-1 cell lines were purchased from ATCC (USA).
Techniques: Biomarker Discovery, Expressing, CCK-8 Assay, Transwell Assay, Migration