Journal: Bone Research

Article Title: Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

doi: 10.1038/s41413-023-00265-w

Figure Lengend Snippet: Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm

Article Snippet: hFOB cells (Procell) were cultured at 34 °C in a 5% CO 2 incubator in Dulbecco’s modified Eagle’s medium/F12 supplemented with 10% fetal bovine serum (FBS).

Techniques: Transformation Assay, Expressing, Quantitative Proteomics, Western Blot, Staining, Cell Culture, Derivative Assay

Journal: International Journal of Nanomedicine

Article Title: Electrospun nanofiber blend with improved mechanical and biological performance

doi: 10.2147/IJN.S175619

Figure Lengend Snippet: ( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of osteoblasts. ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.

Article Snippet: Totally 5,000 cells/cm 2 of human osteoblast cells line (hFOB 1.19, bone-forming cells; Lonza, CRL-11372, second passage) were cultured using C27015 media (Osteoblast Basal Medium, Promocell GmbH), 1% penicillin/streptomycin (P/S; Hyclone), and an Osteoblast Growth Medium Supplement Mix under standard cell culture conditions (37°C, 5% CO 2 , and 95% air).

Techniques: Activity Assay, MTS Assay, Control, Produced, Electron Microscopy

Journal: Journal of Clinical Laboratory Analysis

Article Title: COLEC12 regulates apoptosis of osteosarcoma through Toll‐like receptor 4–activated inflammation

doi: 10.1002/jcla.23469

Figure Lengend Snippet: A, Western blot assay of COLEC12 expression in tumor and para‐tumor tissue. B, Western blot assay of COLEC12 expression in hFOB, 143B, MG‐63, Saos‐2, and HOS cells. C, Quantification. and D, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different tissues. E, Quantification. and F, Quantitative RT‐PCR assay for mRNA level of COLEC12 in different cells. Protein and mRNA levels were normalized to β‐actin. (tumor or Saos‐2 cells vs. para‐tumor or hFOB cells, * P < .05, n = 6 per group; all data were represented as mean ± standard error)

Article Snippet: The following reagents and antibodies were used in this study: hFOB cells (CL‐0353, Procell), 143B cells (CRL‐8303, ATCC), MG‐63 cells (CL‐0157, Procell), Saos‐2 cells (CL‐0202, Procell), and HOS cells (CL‐0360, Procell).

Techniques: Western Blot, Expressing, Quantitative RT-PCR