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human dermal fibroblast hdf cells  (ATCC)


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    Structured Review

    ATCC human dermal fibroblast hdf cells
    Human Dermal Fibroblast Hdf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1824 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hdfs/10__3390_slash_polym18101209-95-7-16?v=ATCC
    Average 99 stars, based on 1824 article reviews
    human dermal fibroblast hdf cells - by Bioz Stars, 2026-06
    99/100 stars

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    Isolation and characterization of chorionic plate mesenchymal stem cell (CP-MSC)-derived sEVs. (A) Schematic showing the isolation and characterization of sEVs derived from <t>CP-MSCs</t> <t>cultured</t> under O₂ Nor , O₂ Hypo-5% , or O₂ Hypo-3% conditions. (B) TEM images showing the typical bilayer structure of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% ; scale bar = 100 nm. (C) Nanoparticle tracking analysis (NTA) profiles showing the size distribution and concentration of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% after 400-fold dilution. (D) Western blotting analysis showing the expression of CD9, CD63, TSG101, and Calnexin proteins in these 3 sEVs. (E) Confocal microscopy images showing the internalization of Dil-labeled sEVs by high-glucose human umbilical vein endothelial cells (HG-HUVECs) and high-glucose human dermal fibroblasts <t>(HG-HDFs);</t> scale bar = 100 μm.
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    Isolation and characterization of chorionic plate mesenchymal stem cell (CP-MSC)-derived sEVs. (A) Schematic showing the isolation and characterization of sEVs derived from <t>CP-MSCs</t> <t>cultured</t> under O₂ Nor , O₂ Hypo-5% , or O₂ Hypo-3% conditions. (B) TEM images showing the typical bilayer structure of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% ; scale bar = 100 nm. (C) Nanoparticle tracking analysis (NTA) profiles showing the size distribution and concentration of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% after 400-fold dilution. (D) Western blotting analysis showing the expression of CD9, CD63, TSG101, and Calnexin proteins in these 3 sEVs. (E) Confocal microscopy images showing the internalization of Dil-labeled sEVs by high-glucose human umbilical vein endothelial cells (HG-HUVECs) and high-glucose human dermal fibroblasts <t>(HG-HDFs);</t> scale bar = 100 μm.
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    Image Search Results


    Isolation and characterization of chorionic plate mesenchymal stem cell (CP-MSC)-derived sEVs. (A) Schematic showing the isolation and characterization of sEVs derived from CP-MSCs cultured under O₂ Nor , O₂ Hypo-5% , or O₂ Hypo-3% conditions. (B) TEM images showing the typical bilayer structure of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% ; scale bar = 100 nm. (C) Nanoparticle tracking analysis (NTA) profiles showing the size distribution and concentration of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% after 400-fold dilution. (D) Western blotting analysis showing the expression of CD9, CD63, TSG101, and Calnexin proteins in these 3 sEVs. (E) Confocal microscopy images showing the internalization of Dil-labeled sEVs by high-glucose human umbilical vein endothelial cells (HG-HUVECs) and high-glucose human dermal fibroblasts (HG-HDFs); scale bar = 100 μm.

    Journal: Research

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells

    doi: 10.34133/research.1248

    Figure Lengend Snippet: Isolation and characterization of chorionic plate mesenchymal stem cell (CP-MSC)-derived sEVs. (A) Schematic showing the isolation and characterization of sEVs derived from CP-MSCs cultured under O₂ Nor , O₂ Hypo-5% , or O₂ Hypo-3% conditions. (B) TEM images showing the typical bilayer structure of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% ; scale bar = 100 nm. (C) Nanoparticle tracking analysis (NTA) profiles showing the size distribution and concentration of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% after 400-fold dilution. (D) Western blotting analysis showing the expression of CD9, CD63, TSG101, and Calnexin proteins in these 3 sEVs. (E) Confocal microscopy images showing the internalization of Dil-labeled sEVs by high-glucose human umbilical vein endothelial cells (HG-HUVECs) and high-glucose human dermal fibroblasts (HG-HDFs); scale bar = 100 μm.

    Article Snippet: HDFs (ATCC, CRL-4053, USA) were cultured in DMEM, containing 10% FBS, 1% PS, and 50 mM glucose for 60 d.

    Techniques: Isolation, Derivative Assay, Cell Culture, Concentration Assay, Western Blot, Expressing, Confocal Microscopy, Labeling