Journal: Research

Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells

doi: 10.34133/research.1248

Figure Lengend Snippet: Isolation and characterization of chorionic plate mesenchymal stem cell (CP-MSC)-derived sEVs. (A) Schematic showing the isolation and characterization of sEVs derived from CP-MSCs cultured under O₂ Nor , O₂ Hypo-5% , or O₂ Hypo-3% conditions. (B) TEM images showing the typical bilayer structure of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% ; scale bar = 100 nm. (C) Nanoparticle tracking analysis (NTA) profiles showing the size distribution and concentration of sEVs Nor , sEVs Hypo-5% , and sEVs Hypo-3% after 400-fold dilution. (D) Western blotting analysis showing the expression of CD9, CD63, TSG101, and Calnexin proteins in these 3 sEVs. (E) Confocal microscopy images showing the internalization of Dil-labeled sEVs by high-glucose human umbilical vein endothelial cells (HG-HUVECs) and high-glucose human dermal fibroblasts (HG-HDFs); scale bar = 100 μm.

Article Snippet: HDFs (ATCC, CRL-4053, USA) were cultured in DMEM, containing 10% FBS, 1% PS, and 50 mM glucose for 60 d.

Techniques: Isolation, Derivative Assay, Cell Culture, Concentration Assay, Western Blot, Expressing, Confocal Microscopy, Labeling