h37rv  (ATCC)

Journal: bioRxiv

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells

doi: 10.64898/2026.05.05.723095

Figure Lengend Snippet: (A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis Triton X-114 extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).

Article Snippet: The M. tuberculosis (H37Rv) Triton X-114 extracted proteins (50 mg) were lyophilized with 7.25 M PlusOne urea (Fisher), 0.4% 3-10 ZOOM carrier ampholytes (ThermoFisher), 1.6% 4-7 pharmalytes, 1% N-octylthioglucoside (EMD Chemicals, Inc) and 2 mM DL-Dithiothreitol (DTT, Fisher).

Techniques: Incubation

Journal: bioRxiv

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells

doi: 10.64898/2026.05.05.723095

Figure Lengend Snippet: (A) The M. tuberculosis Triton X-114 extract was subjected to preparative IEF, and each fraction was analyzed by Western blot and screened for T cell activation. (B) Identification of candidate proteins by proteomic analysis (LC–MS/MS) of the active fractions. Active fractions stimulating each T cell line were identified by T cell “Western blot” assay. (C) Proliferative response of LCD4.G to purified recombinant lipoproteins derived from the 25-kDa M. tuberculosis lipoglycoprotein LppX. mTB son, M. tuberculosis sonicated. mSMEG, M. smegmatis . Data indicate mean of triplicate values for LCD4.G T cell line and are representative of three independent experiments (A–C). See also Figures S2 and S5.

Article Snippet: The M. tuberculosis (H37Rv) Triton X-114 extracted proteins (50 mg) were lyophilized with 7.25 M PlusOne urea (Fisher), 0.4% 3-10 ZOOM carrier ampholytes (ThermoFisher), 1.6% 4-7 pharmalytes, 1% N-octylthioglucoside (EMD Chemicals, Inc) and 2 mM DL-Dithiothreitol (DTT, Fisher).

Techniques: Western Blot, Activation Assay, Liquid Chromatography with Mass Spectroscopy, Purification, Recombinant, Derivative Assay, Sonication

Journal: medRxiv

Article Title: Direct detection and quantification of Mycobacterium tuberculosis from clinical samples by high-resolution melt qPCR

doi: 10.64898/2026.03.07.26347851

Figure Lengend Snippet: (A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Article Snippet: Using the M. tuberculosis H37Rv (ATCC #25618DQ) reference strain, we performed asymmetric PCR with Vent (exo-) DNA polymerase (New England Biolabs).

Techniques: Amplification, Control, Generated