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mycobacterium tuberculosis h37rv  (ATCC)
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Mycobacterium Tuberculosis H37rv, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m tuberculosis h37rv triton x 114 extracted proteins  (Thermo Fisher)
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(A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis <t>Triton</t> <t>X-114</t> extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).
M Tuberculosis H37rv Triton X 114 Extracted Proteins, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium tuberculosis strain h37rv genome  (Biotechnology Information)
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(A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis <t>Triton</t> <t>X-114</t> extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).
Mycobacterium Tuberculosis Strain H37rv Genome, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis <t>Triton</t> <t>X-114</t> extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).
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cell wall reporter assay 8 mycobacterium tuberculosis h37rv expressing pini lux8  (Bacto Laboratories)
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(A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis <t>Triton</t> <t>X-114</t> extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).
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virus strains m tuberculosis h37rv lab stock atcc 27294 m tuberculosis h37ra lab stock atcc 25177 cell lines hepg2 cells  (ATCC)
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(A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis <t>Triton</t> <t>X-114</t> extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).
Virus Strains M Tuberculosis H37rv Lab Stock Atcc 27294 M Tuberculosis H37ra Lab Stock Atcc 25177 Cell Lines Hepg2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m tuberculosis h37rv  (ATCC)
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ATCC m tuberculosis h37rv
(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
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m tuberculosis h37rv strain  (ATCC)
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ATCC m tuberculosis h37rv strain
(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
M Tuberculosis H37rv Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium tuberculosis h37rv  (Pasteur Institute)
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Pasteur Institute mycobacterium tuberculosis h37rv
(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
Mycobacterium Tuberculosis H37rv, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis Triton X-114 extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).

Journal: bioRxiv

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells

doi: 10.64898/2026.05.05.723095

Figure Lengend Snippet: (A) LCD4.G, LCD4.C, and LCD4.D T cell lines responses to M. tuberculosis (mTB son), M. leprae (mLEP son), M. smegmatis (mSMEG) and non-mycobacterial lysates. (B) LCD4.G and LCD4.CD T cell lines were tested for recognition of known CD1-presented antigens, including dideoxymycobactin (DDM), glucose monomycolate (GMM), mycolic acid, mannose-capped lipoarabinomannan (manLAM), and mannosyl phosphodolichol (MPD). (C) Effect of proteinase K and heat treatment of the M. tuberculosis Triton X-114 extract on T cell proliferation. TX-114 extract was added to the T cell assay after one of four treatments: untreated; proteinase K treatment followed by heat inactivation; heat treatment alone; or incubation with heat-inactivated proteinase K. Data indicate mean ± SEM of triplicate values and are representative of three independent experiments (A–C).

Article Snippet: The M. tuberculosis (H37Rv) Triton X-114 extracted proteins (50 mg) were lyophilized with 7.25 M PlusOne urea (Fisher), 0.4% 3-10 ZOOM carrier ampholytes (ThermoFisher), 1.6% 4-7 pharmalytes, 1% N-octylthioglucoside (EMD Chemicals, Inc) and 2 mM DL-Dithiothreitol (DTT, Fisher).

Techniques: Incubation

(A) The M. tuberculosis Triton X-114 extract was subjected to preparative IEF, and each fraction was analyzed by Western blot and screened for T cell activation. (B) Identification of candidate proteins by proteomic analysis (LC–MS/MS) of the active fractions. Active fractions stimulating each T cell line were identified by T cell “Western blot” assay. (C) Proliferative response of LCD4.G to purified recombinant lipoproteins derived from the 25-kDa M. tuberculosis lipoglycoprotein LppX. mTB son, M. tuberculosis sonicated. mSMEG, M. smegmatis . Data indicate mean of triplicate values for LCD4.G T cell line and are representative of three independent experiments (A–C). See also Figures S2 and S5.

Journal: bioRxiv

Article Title: CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells

doi: 10.64898/2026.05.05.723095

Figure Lengend Snippet: (A) The M. tuberculosis Triton X-114 extract was subjected to preparative IEF, and each fraction was analyzed by Western blot and screened for T cell activation. (B) Identification of candidate proteins by proteomic analysis (LC–MS/MS) of the active fractions. Active fractions stimulating each T cell line were identified by T cell “Western blot” assay. (C) Proliferative response of LCD4.G to purified recombinant lipoproteins derived from the 25-kDa M. tuberculosis lipoglycoprotein LppX. mTB son, M. tuberculosis sonicated. mSMEG, M. smegmatis . Data indicate mean of triplicate values for LCD4.G T cell line and are representative of three independent experiments (A–C). See also Figures S2 and S5.

Article Snippet: The M. tuberculosis (H37Rv) Triton X-114 extracted proteins (50 mg) were lyophilized with 7.25 M PlusOne urea (Fisher), 0.4% 3-10 ZOOM carrier ampholytes (ThermoFisher), 1.6% 4-7 pharmalytes, 1% N-octylthioglucoside (EMD Chemicals, Inc) and 2 mM DL-Dithiothreitol (DTT, Fisher).

Techniques: Western Blot, Activation Assay, Liquid Chromatography with Mass Spectroscopy, Purification, Recombinant, Derivative Assay, Sonication

(A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Journal: medRxiv

Article Title: Direct detection and quantification of Mycobacterium tuberculosis from clinical samples by high-resolution melt qPCR

doi: 10.64898/2026.03.07.26347851

Figure Lengend Snippet: (A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Article Snippet: Using the M. tuberculosis H37Rv (ATCC #25618DQ) reference strain, we performed asymmetric PCR with Vent (exo-) DNA polymerase (New England Biolabs).

Techniques: Amplification, Control, Generated