mouse spermatogonial gc1  (ATCC)

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Determination of the IC50 values (μ m ) for GC1 and GC2 cell lines following 24 h SAL exposure. Representative phase‐contrast images demonstrate the typical epithelioid morphology of cells maintained under standard culture conditions and the reduced viability observed at the IC50 concentration of SAL. The cell images on the left side represent control (C) cells, while the images on the right side represent cells treated with IC50 concentrations of SAL (IC50 SAL). Black arrow: Living cell. White arrow: Dead cell. Scale bar: 100 μm.

Article Snippet: The mouse spermatogonial GC1 (ATCC® CRL‐2053TM Manassas, VA, USA) and GC2 (ATCC® CRL‐2196TM Manassas, VA, USA) cell lines used in the study were purchased commercially from ATCC (USA).

Techniques: Concentration Assay, Control

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Inverted microscope images of GC1 and GC2 experimental groups. Quantitative analysis shows a decrease in viable cell numbers in the HSM‐applied groups compared to controls. Data are expressed as mean ± SD ( n = 3, 10 randomly selected fields per group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (** P < 0.05; statistical analysis was performed using graphpad Software). Scale bar: 100 μm.

Article Snippet: The mouse spermatogonial GC1 (ATCC® CRL‐2053TM Manassas, VA, USA) and GC2 (ATCC® CRL‐2196TM Manassas, VA, USA) cell lines used in the study were purchased commercially from ATCC (USA).

Techniques: Inverted Microscopy, Software

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC1 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields/group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Article Snippet: The mouse spermatogonial GC1 (ATCC® CRL‐2053TM Manassas, VA, USA) and GC2 (ATCC® CRL‐2196TM Manassas, VA, USA) cell lines used in the study were purchased commercially from ATCC (USA).

Techniques: Staining, Generated, Fluorescence, Software

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Relative mRNA expression levels of GRP78 (A), PERK (B), and eIF2α (C) in GC1 and GC2 cells. Gene expression was quantified by qRT‐PCR and analyzed using the 2 − Δ Δ C t method. The control groups (GC1C and GC2C) were designated as calibrators and normalized to a fixed value of 1.0 by definition. Data are presented as mean ± SD from three independent biological replicates, each analyzed in technical triplicates. Individual data points are shown for all groups. Statistical evaluation was performed using the Kruskal–Wallis test, followed by Dunn's post hoc test (* P < 0.05; statistical analysis was performed using graphpad Software).

Article Snippet: The mouse spermatogonial GC1 (ATCC® CRL‐2053TM Manassas, VA, USA) and GC2 (ATCC® CRL‐2196TM Manassas, VA, USA) cell lines used in the study were purchased commercially from ATCC (USA).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Control, Software

Journal: Cell reports

Article Title: mRNA decay pre-complex assembly drives timely cell-state transitions during differentiation

doi: 10.1016/j.celrep.2024.115138

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: GC1-RB -B2 , Molecular Instruments , GenBank: NM_001275561.

Techniques: Recombinant, Staining, Software

Journal: bioRxiv

Article Title: REEP6 deficiency impairs ER and Golgi morphologies and causes retinal degeneration by attenuating the expression of phototransduction proteins

doi: 10.1101/2025.03.02.641069

Figure Lengend Snippet: ( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, GC2, GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.

Article Snippet: The sections were immunostained as previously described using primary antibodies against the following proteins: rhodopsin (1:1,000, custom-made ( )), GC1 (1:200, Proteintech, China), GC2 (1:500, a gift from Dr. Wolfgang Baehr at the University of Utah), PDE6B (1:500, Proteintech, China), GRK1 (1:200, Proteintech, China), and GCAP2 (1:500, a gift from Dr. Wolfgang Baehr at the University of Utah).

Techniques: Western Blot, Expressing, Control, Immunostaining, Transfection, Recombinant, Staining

Journal: bioRxiv

Article Title: REEP6 deficiency impairs ER and Golgi morphologies and causes retinal degeneration by attenuating the expression of phototransduction proteins

doi: 10.1101/2025.03.02.641069

Figure Lengend Snippet: ( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, GC2, GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.

Article Snippet: The following primary antibodies were used at the given dilution: anti-REEP6 (1:2,000, Proteintech, China), anti-GAPDH (1:10,000, Proteintech, China), anti-rhodopsin (1:10,000, custom-made ( )), anti-GC1 (1:5000, Proteinch, China), anti-GRK1 (1:5,000, Proteintech, China), anti-GC2 (1:5,000, a gift from Dr. Wolfgang Baehr at the University of Utah), and anti-PDE6B (1:5,000, Proteintech, China).

Techniques: Western Blot, Expressing, Control, Immunostaining, Transfection, Recombinant, Staining