Journal: STAR Protocols

Article Title: Protocol for CRISPR-Cas12a genome editing of protein tyrosine phosphatases in human pluripotent stem cells and functional β-like cell generation

doi: 10.1016/j.xpro.2024.103297

Figure Lengend Snippet: Reagents and small molecules used in the β-like cell differentiation

Article Snippet: Sobetirome (GC-1) , Tocris , Cat# 4554.

Techniques: Concentration Assay, Solvent, Recombinant

Journal: STAR Protocols

Article Title: Protocol for CRISPR-Cas12a genome editing of protein tyrosine phosphatases in human pluripotent stem cells and functional β-like cell generation

doi: 10.1016/j.xpro.2024.103297

Figure Lengend Snippet:

Article Snippet: Sobetirome (GC-1) , Tocris , Cat# 4554.

Techniques: Recombinant, Membrane, Knock-Out, Electroporation, Passaging, Transfection, Control, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Pore Size

Journal: bioRxiv

Article Title: REEP6 deficiency impairs ER and Golgi morphologies and causes retinal degeneration by attenuating the expression of phototransduction proteins

doi: 10.1101/2025.03.02.641069

Figure Lengend Snippet: ( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, GC2, GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.

Article Snippet: The sections were immunostained as previously described using primary antibodies against the following proteins: rhodopsin (1:1,000, custom-made ( )), GC1 (1:200, Proteintech, China), GC2 (1:500, a gift from Dr. Wolfgang Baehr at the University of Utah), PDE6B (1:500, Proteintech, China), GRK1 (1:200, Proteintech, China), and GCAP2 (1:500, a gift from Dr. Wolfgang Baehr at the University of Utah).

Techniques: Western Blot, Expressing, Control, Immunostaining, Transfection, Recombinant, Staining

Journal: Advanced Science

Article Title: Deciphering the Metabolic Impact and Clinical Relevance of N‐Glycosylation in Colorectal Cancer through Comprehensive Glycoproteomic Profiling

doi: 10.1002/advs.202415645

Figure Lengend Snippet: Screening for Potential CRC Prognostic Signature. (A) Point plot showing the AUC values for each IGP. Differential colors represent differential N‐Glycoform. Differential size represents the number of values detected in 90 samples. (B) Boxplot illustrating the AUC for each glycoform. Differential colors represent differential N‐Glycoform. (C) Comparison of AUC values for glycoproteins versus non‐glycoproteins. (D) Top 10 glycoproteins with the highest number of IGPs. The color represents the average number of N‐glycan across all sites in each protein, with higher saturation representing a higher average. (E) ROC curve displaying the performance of multiple glycoproteins logistic regression modules in identifying CRC. Each red dot represents an independent ROC value for each protein. (F) ROC curve showing the results of constructing a random forest model using a combination of CLCA1 and OLFM4. Each red dot represents an independent ROC value for each protein. (G) ROC curve presenting the training results of a random forest model for CLCA1 and OLFM4 using public data. Each red dot represents an independent ROC value for each protein. (H) ROC curve illustrating the testing results of a random forest model for CLCA1 and OLFM4 using public data. Each red dot represents an independent ROC value for each protein. (I) Multivariate survival analyses of IHC patients involved Cox regression analysis. The red line segments represent high risk and the blue color represents low risk.

Article Snippet: IHC staining of OLFM4 (1:1000, 28432‐1‐AP, Proteintech) and CLCA1 (1:100, A15041, Abcam) were performed on paraffin‐embedded human colorectal cancer tissue microarray (HColA180Su21, Shanghai Outdo Biotech Co. Ltd., Shanghai, China), as previously described.

Techniques: Comparison, Glycoproteomics