Journal: Fundamental & Clinical Pharmacology
Article Title: Differential Effects of Prenatal Poly I:C Exposure and Antipsychotics on NMDA/GABA Receptors and GSK3β‐Mediated Signaling in the Dorsal Raphe Nucleus of Female Rats
doi: 10.1111/fcp.70033
Figure Lengend Snippet: The effect of prenatal Poly I:C exposure and adult antipsychotic (risperidone and olanzapine) treatment on the mRNA expression of (A) GABA A receptor β1( Gabrb1 ) subunit, (B) GABA A receptor β2 ( Gabrb2 ) subunit, (C) GABA A receptor β3 ( Gabrb3 ) subunit, (D) glutamic acid decarboxylase GAD1 , and (E) glutamic acid decarboxylase GAD2 in the dorsal raphe nuclei of female rats. Data were presented as mean ± SEM, * p < 0.05, # p < 0.01.
Article Snippet: Total RNA from the DRN brain tissue was prepared using the PureLink RNA Mini Kit (#12183025; Invitrogen Life Technologies, Carlsbad, CA, USA). cDNA was synthesized from purified RNA using the High‐Capacity cDNA Reverse Transcription Kits (#4368814; Thermo Fisher Scientific, Waltham, MA, USA). qRT‐PCR was performed in duplicate on a Quant Studio qRT‐PCR system (Thermo Fisher, Waltham, MA, USA) using TaqMan Gene Expression Assays (Life Technologies, Sydney, NSW, Australia) for β‐actin (Hs01060665_g1), Gapdh (Rn01775763_g1), Gabrb1 (Rn00564146_m1), Gabrb2 (Rn00564149_m1), Gad2 (Rn00561244_m1), Grin2c (Rn00561359_m1), and Grin2d (Rn00575638_m1).
Techniques: Expressing