Journal: Cell Cycle

Article Title: Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells

doi: 10.1080/15384101.2022.2103342

Figure Lengend Snippet: Changes in the expression profile of genes differentiating keratinocyte cultures exposed to LPS and cyclosporin A compared to the controls (p < 0.05; −2.0 < FC> +2.0 at least in one comparison).

Article Snippet: The following specific primers (Thermo Fisher Scientific, USA) were used for qRT-PCR analysis: HRH1 , Hs00185542_m1; HRH2 , Hs00254569_s1; HRH3 , Hs00200610_m1; GABRB1 , Hs00181306_m1; GABRB2 , Hs00241451_m1, GABRB3 , Hs00241459_m1; HTMT , Hs02759756_s1); ACTB , Hs03023943_g1. β-actine ( ACTB) served as an endogenous control.

Techniques: Expressing, Comparison

Journal: Cell Cycle

Article Title: Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells

doi: 10.1080/15384101.2022.2103342

Figure Lengend Snippet: Changes in the expression profile of genes differentiating keratinocyte cultures exposed to LPS and Adalimumab compared to the controls (p < 0.05; −2.0 < FC> +2.0 at least in one comparison).

Article Snippet: The following specific primers (Thermo Fisher Scientific, USA) were used for qRT-PCR analysis: HRH1 , Hs00185542_m1; HRH2 , Hs00254569_s1; HRH3 , Hs00200610_m1; GABRB1 , Hs00181306_m1; GABRB2 , Hs00241451_m1, GABRB3 , Hs00241459_m1; HTMT , Hs02759756_s1); ACTB , Hs03023943_g1. β-actine ( ACTB) served as an endogenous control.

Techniques: Expressing, Comparison

Journal: Cell Cycle

Article Title: Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells

doi: 10.1080/15384101.2022.2103342

Figure Lengend Snippet: Quantitative real-time polymerase chain reaction analysis of the mRNA expression patterns of HRH1, HRH2, HRH3, HNMT, GABRB1, GABRB2, and GABRB3 in lipopolysaccharide (LPS)-stimulated/Adalimumab-treated and control HaCaT cells.

Article Snippet: The following specific primers (Thermo Fisher Scientific, USA) were used for qRT-PCR analysis: HRH1 , Hs00185542_m1; HRH2 , Hs00254569_s1; HRH3 , Hs00200610_m1; GABRB1 , Hs00181306_m1; GABRB2 , Hs00241451_m1, GABRB3 , Hs00241459_m1; HTMT , Hs02759756_s1); ACTB , Hs03023943_g1. β-actine ( ACTB) served as an endogenous control.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Cell Cycle

Article Title: Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells

doi: 10.1080/15384101.2022.2103342

Figure Lengend Snippet: Quantitative real-time polymerase chain reaction analysis of mRNA expression patterns of HRH1, HRH2, HRH3, HNMT, GABRB1, GABRB2, and GABRB3 in lipopolysaccharide-stimulated/cyclosporine A-treated HaCaT cells.

Article Snippet: The following specific primers (Thermo Fisher Scientific, USA) were used for qRT-PCR analysis: HRH1 , Hs00185542_m1; HRH2 , Hs00254569_s1; HRH3 , Hs00200610_m1; GABRB1 , Hs00181306_m1; GABRB2 , Hs00241451_m1, GABRB3 , Hs00241459_m1; HTMT , Hs02759756_s1); ACTB , Hs03023943_g1. β-actine ( ACTB) served as an endogenous control.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Translational Psychiatry

Article Title: Gabrb2 -knockout mice displayed schizophrenia-like and comorbid phenotypes with interneuron–astrocyte–microglia dysregulation

doi: 10.1038/s41398-018-0176-9

Figure Lengend Snippet: a Tail-suspension test showing immobility time of mice suspended by the tail to a horizontal bar (WT male n = 16, HT male n = 15, KO male n = 12). b Sucrose-preference test showing preference in terms of sucrose solution consumed as percentage of total liquid consumption (WT male n = 14, HT male n = 15, KO male n = 12). c Elevated-plus maze test showing percentile entries into open arms (WT male n = 12, HT male n = 15, KO male n = 14). In schematic of ( c) , O represents open arm and C represents closed arm. d Schematic of the different phases in audiogenic epilepsy. e KO mouse undergoing tonic seizure. f Audiogenic epilepsy: significant effect of gender on latency of audiogenic epilepsy was displayed by the mice ( F 1,65 = 12.12, p < 0.01; week-3 KO male n = 18, female n = 19; week-10 KO male n = 16, female n = 16). g PTZ-induced seizure: significant effects of gender ( F 1,57 = 6.12, p < 0.05) and genotype ( F 2,57 = 4.71, p < 0.05) on the latency of PTZ-induced seizure were displayed by the mice (WT male n = 11, female n = 11; HT male n = 10, female n = 10; KO male n = 11, female n = 10). In both parts ( f) and (g) , male and female mice are labeled M and F, respectively; and data were analyzed using two-way ANOVA with Dunnett’s post-hoc test. h Schematic representation of two types of HT hemizygosity differing in parental origin of undeleted copy of Gabrb2 . Upper: HT-P mouse displayed the phenotype of the Gabrb2 of paternal origin (light green) while the maternal copy (pink) had been deleted. Lower: HT-M mouse displayed the phenotype of the Gabrb2 of maternal origin (pink) while the paternal copy (light green) had been deleted. i Imprinting effect on locomotor activity (WT male n = 17, HT-P male n = 15, HT-M male n = 17, KO male n = 22). j Imprinting effect on behavioral stereotypy (WT male n = 17, HT-P male n = 15, HT-M male n = 17, KO male n = 22). Except for the epilepsy tests ( f , g ), statistical analysis was performed using one-way ANOVA with Newman–Keuls post-hoc test. WT mice are represented by green dots; HT by orange dots; HT-P by light green dots; HT-M by pink dots; and KO by red dots. Average y values ± SEM in the different plots are represented by horizontal bars. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Gabrb2 HT transgenic mice (C57BL/6-129/SvEv hybrid) supplied by Taconic Farms, Inc. (New York) were bred to yield WT, HT, and KO mice that were weaned at week-3, genotyped using primers specific for the Gabrb2 and Neo genes (Supplementary Methods) and housed in groups of four to six with water and food ad lib, with a 12-h light cycle from 08:00 to 20:00.

Techniques: Suspension, Labeling, Activity Assay