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dlgap5  (Proteintech)


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    Proteintech dlgap5
    Dlgap5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dlgap5/pm40962169-79-3-26?v=Proteintech
    Average 93 stars, based on 11 article reviews
    dlgap5 - by Bioz Stars, 2026-07
    93/100 stars

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    Analysis of cell trajectory, subpopulation abundance, and gene expression in glioma. A , B Cell trajectory analysis using the CytoTRACE algorithm. The inferred pseudotime map visualizes the differentiation potential of glioma-related subpopulations, with darker shades indicating less differentiated (more stem-like) cells and lighter shades indicating more differentiated states. The trajectory highlights the pivotal position of oligodendrocytes at a branching differentiation point, suggesting their potential role in glioma progression. C Abundance proportions of different cell subpopulations in control and glioma groups, with notable increases in T cells, proliferating cancer cells, and inhibitory neurons, and reductions in oligodendrocytes, astrocytes, and tumor-associated macrophages (TAM) in the glioma group. D Expression patterns of specific genes (CHEK1, DLGAP5, MKI67, and TOP2A) across different cell subpopulations, with high expression observed in proliferating cancer cells

    Journal: Discover Oncology

    Article Title: Identification of key biomarkers and immune microenvironment features in gliomas based on single-cell analysis combined with bioinformatics

    doi: 10.1007/s12672-025-03450-x

    Figure Lengend Snippet: Analysis of cell trajectory, subpopulation abundance, and gene expression in glioma. A , B Cell trajectory analysis using the CytoTRACE algorithm. The inferred pseudotime map visualizes the differentiation potential of glioma-related subpopulations, with darker shades indicating less differentiated (more stem-like) cells and lighter shades indicating more differentiated states. The trajectory highlights the pivotal position of oligodendrocytes at a branching differentiation point, suggesting their potential role in glioma progression. C Abundance proportions of different cell subpopulations in control and glioma groups, with notable increases in T cells, proliferating cancer cells, and inhibitory neurons, and reductions in oligodendrocytes, astrocytes, and tumor-associated macrophages (TAM) in the glioma group. D Expression patterns of specific genes (CHEK1, DLGAP5, MKI67, and TOP2A) across different cell subpopulations, with high expression observed in proliferating cancer cells

    Article Snippet: HRP-conjugated goat anti-rabbit IgG (S0001), TOP2A antibody (AF0793), DLGAP5 antibody (DF6920), and Chk1 antibody (AF6007) were purchased from Affinity Biosciences Ltd (USA).

    Techniques: Gene Expression, Control, Expressing

    Molecular docking analysis of quercetin (QU) with key target genes. A Chemical structure of QU. B–E Docking interactions between quercetin and the four target genes (CHEK1, DLGAP5, MKI67, and TOP2A), visualized using PyMOL software. All four interactions show binding energies below − 5 kJ/mol, indicating strong binding affinities

    Journal: Discover Oncology

    Article Title: Identification of key biomarkers and immune microenvironment features in gliomas based on single-cell analysis combined with bioinformatics

    doi: 10.1007/s12672-025-03450-x

    Figure Lengend Snippet: Molecular docking analysis of quercetin (QU) with key target genes. A Chemical structure of QU. B–E Docking interactions between quercetin and the four target genes (CHEK1, DLGAP5, MKI67, and TOP2A), visualized using PyMOL software. All four interactions show binding energies below − 5 kJ/mol, indicating strong binding affinities

    Article Snippet: HRP-conjugated goat anti-rabbit IgG (S0001), TOP2A antibody (AF0793), DLGAP5 antibody (DF6920), and Chk1 antibody (AF6007) were purchased from Affinity Biosciences Ltd (USA).

    Techniques: Software, Binding Assay

    Effects of quercetin on cell viability and protein expression in glioma cells. A CCK-8 assay showing the viability of U87MG cells treated with increasing concentrations of quercetin (0, 10, 25, 50, 100, 150, 200, 250 µM) for 24 h. Quercetin significantly reduced cell viability in a dose-dependent manner, with approximately 50% inhibition observed at 100 µM. B Western blot analysis of CHEK1, DLGAP5, and TOP2A protein expression in the control group (HEB), model group (U87MG), and QU group (U87MG + 100 µM quercetin). C Quantitative analysis of protein band intensity. Quercetin treatment significantly downregulated the expression of target proteins compared to untreated U87MG cells. β-actin was used as the internal control. Data are presented as mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Discover Oncology

    Article Title: Identification of key biomarkers and immune microenvironment features in gliomas based on single-cell analysis combined with bioinformatics

    doi: 10.1007/s12672-025-03450-x

    Figure Lengend Snippet: Effects of quercetin on cell viability and protein expression in glioma cells. A CCK-8 assay showing the viability of U87MG cells treated with increasing concentrations of quercetin (0, 10, 25, 50, 100, 150, 200, 250 µM) for 24 h. Quercetin significantly reduced cell viability in a dose-dependent manner, with approximately 50% inhibition observed at 100 µM. B Western blot analysis of CHEK1, DLGAP5, and TOP2A protein expression in the control group (HEB), model group (U87MG), and QU group (U87MG + 100 µM quercetin). C Quantitative analysis of protein band intensity. Quercetin treatment significantly downregulated the expression of target proteins compared to untreated U87MG cells. β-actin was used as the internal control. Data are presented as mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: HRP-conjugated goat anti-rabbit IgG (S0001), TOP2A antibody (AF0793), DLGAP5 antibody (DF6920), and Chk1 antibody (AF6007) were purchased from Affinity Biosciences Ltd (USA).

    Techniques: Expressing, CCK-8 Assay, Inhibition, Western Blot, Control