dapt (Tocris)

Tocris is a verified supplier

Tocris manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Tocris dapt
Dapt, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapt/DAPT/pm42885744-141-41-42
Average 96 stars, based on 534 article reviews

dapt - by Bioz Stars, 2026-11

96/100 stars

Images

other:

Article Title: iPSC-induced multilineage liver organoids, small intestinal organoids and brain organoids sustain pangenotype hepatitis E virus propagation
Article Snippet: Next, the cells were cultured with posterior foregut medium for another 4 days: 100 ng/mL Activin A, 1 ng/mL bone morphogenetic protein 4 (BMP4), 5 ng/mL fibroblast growth factor 2 (FGF2, R&D Systems, USA) and 10 ng/mL vascular endothelial factor (VEGF, Peprotech, USA) in RPMI 1640 supplemented with 1×GlutaMAX, 1×NB27 (iORGANtech, China), and 1×NB2 (iORGANtech, China).

Recombinant:

Article Title: Dissecting the transcriptome of Parkin (PRKN)-linked Parkinson’s disease using 2D and 3D hiPSC-neuronal models
Article Snippet: During days 7 to 10 of differentiation, increasing amounts of Neurobasal medium (Gibco) plus B27 supplement (Gibco) were added to the KSR medium (25%, 50%, and 75%). .. On day 12, maturation of mDA neurons was initiated by adding recombinant Human BDNF (Peprotech, 20 ng/ml), recombinant Human GDNF (Peprotech, 20 ng/ml), ascorbic acid (AA, Sigma-Aldrich, 0.2 mM), recombinant Human TGF-beta 3 (ß3, Peprotech, 1 ng/ml), dibutyryl-cyclic-AMP (dbcAMP, Sigma-Aldrich, 0.5 mM), and DAPT (Tocris, 10 μM). .. On day 20 of differentiation, cells differentiated in 2D were passaged en bloc or as single cells on 24-well plates coated with Poly-D-lysine (Sigma-Aldrich) and Laminin (Sigma-Aldrich) (PDL/LA).

Cell Culture:

Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.
Article Snippet: On day 7, spheres were maintained in culture with RA and Smoothened agonist, supplemented with recombinant human brain-derived neurotrophic factor (BDNF; 10 ng ml−1; R&D Systems) in N2/B27 medium to generate MN progenitors. .. From day 9, MN progenitors were cultured in the day 7 medium with the addition of DAPT (10 μM; Tocris Bio-Techne), an inhibitor of Notch signaling, for an additional 5–7 days. .. At days 14–16, MN spheres were dissociated using 0.05% trypsin–EDTA (Gibco, Thermo Fisher Scientific), and cells were plated as a monolayer onto dishes coated with laminin from Engelbreth–Holm–Swarm mouse sarcoma basement membrane (5 μg ml−1; Sigma-Aldrich), fibronectin human plasma (10 μg ml−1; Sigma-Aldrich) and Matrigel (1:20), which had been pretreated with poly-L-ornithine bromide (100 μg ml−1; Sigma-Aldrich).

Article Title: Spatial modelling of NOTCH signalling predicts tuneable dynamic heterogeneity in endothelia
Article Snippet: For experiments, 4 × 10 4 ECs per well were seeded for confluent experiments and 1 × 10 3 ECs per well for sparse experiments in fibronectin-coated 96-well plates (μClear, Greiner). .. Cells were cultured for 24 h for sparse and 96 h for confluent under basal conditions (EGMV2, Promocell) or treated with DAPT (5 μM, Tocris bioscience, UK). ..

Inhibition:

Article Title: Divergent consequences of PSEN1 knockout and PSEN2 knockout in stem cell derived models of the brain
Article Snippet: .. Day 100 was taken as the final time point for mature cortical glutamatergic neuronal cultures. γ-secretase inhibition was achieved using 10μM DAPT (Tocris) treatment for 48 hours. iPSCs were differentiated to microglia-like cells (iMGLCs) using published protocols [ , ] previously established [ ]. ..

Review