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dihydrochloride dapi staining  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dihydrochloride dapi staining
    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Dihydrochloride Dapi Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/4'%2C6'-DIAMIDINO-2-PHENYLINDOLE+DIHYDROCHLORIDE/pmc13112262-66-41-44
    Average 97 stars, based on 1 article reviews
    dihydrochloride dapi staining - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold"

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    Journal: Biomaterials and Biosystems

    doi: 10.1016/j.bbiosy.2026.100137

    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
    Figure Legend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Techniques Used: Staining, Cell Culture

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    Staining:

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    Article Snippet: Non-specific binding in vWF staining was prevented by incubation with mouse Fc-blocking solution (1:200, BD Biosciences) for 1 h. Isotype controls were applied to confirm specificity of primary antibodies. .. Sections were washed and incubated with fluorochrome-conjugated secondary antibodies (Table S3) for 1 h in the dark, followed by DAPI staining (1:1000 in PBS, Thermo Fisher Scientific) for 15 min. Autofluorescence of elastic fibers was suppressed using the TrueVIEW autofluorescence quenching Kit (Vector Laboratories, Newark, USA). .. Coverslips weremounted onto glass slideswith a fluorescencemountingmedium (Dako, Agilent, Santa-Clara, USA) and imaged using the BZ-9000microscope (Keyence, Osaka, Japan).

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    Article Snippet: For IF, slides were incubated with fluorescent secondary antibodies 1:500 (Alexa Flour 488, A21121 and Alexa Flour 546, A11035, Invitrogen) for 1 h at room temperature. .. Subsequently, slides underwent a 15-minute PBS wash. To visualize cell nuclei, DAPI staining (Thermo Scientific, product number 62248, diluted 1:1000) was applied for 3 min at ambient temperature, followed by an additional 3-minute PBS rinse. .. Anti-fade fluorescence mounting medium (Abcam, catalog number ab104135) was applied and slides were stored in light-protected containers at 4 °C.

    Article Title: Myeloperoxidase impacts vascular function by altering perivascular adipocytes’ secretome and phenotype in obesity
    Article Snippet: Non-specific binding in vWF staining was prevented by incubation with mouse Fc-blocking solution (1:200, BD Biosciences) for 1 h. Isotype controls were applied to confirm specificity of primary antibodies. .. Sections were washed and incubated with fluorochrome-conjugated secondary antibodies ( ) for 1 h in the dark, followed by DAPI staining (1:1000 in PBS, Thermo Fisher Scientific) for 15 min. Autofluorescence of elastic fibers was suppressed using the TrueVIEW autofluorescence quenching Kit (Vector Laboratories, Newark, USA). .. Coverslips were mounted onto glass slides with a fluorescence mounting medium (Dako, Agilent, Santa-Clara, USA) and imaged using the BZ-9000 microscope (Keyence, Osaka, Japan).

    Article Title: Pathogenic germline variants in Chinese pancreatic adenocarcinoma patients
    Article Snippet: A subsequent 1-h incubation was performed using the secondary goat-anti-rabbit Alexa-Fluor 488 antibody (ab150141, Abcam), also diluted 1:200 in the blocking buffer. .. Additionally, DAPI staining (Thermo Fisher Scientific) was applied to PANC-1 cells. .. Captured images were obtained using a Zeiss LSM 900 confocal microscope, with uniform settings maintained across all acquired images.

    Incubation:

    Article Title: Myeloperoxidase impacts vascular function by altering perivascular adipocytes' secretome and phenotype in obesity.
    Article Snippet: Non-specific binding in vWF staining was prevented by incubation with mouse Fc-blocking solution (1:200, BD Biosciences) for 1 h. Isotype controls were applied to confirm specificity of primary antibodies. .. Sections were washed and incubated with fluorochrome-conjugated secondary antibodies (Table S3) for 1 h in the dark, followed by DAPI staining (1:1000 in PBS, Thermo Fisher Scientific) for 15 min. Autofluorescence of elastic fibers was suppressed using the TrueVIEW autofluorescence quenching Kit (Vector Laboratories, Newark, USA). .. Coverslips weremounted onto glass slideswith a fluorescencemountingmedium (Dako, Agilent, Santa-Clara, USA) and imaged using the BZ-9000microscope (Keyence, Osaka, Japan).

    Article Title: Myeloperoxidase impacts vascular function by altering perivascular adipocytes’ secretome and phenotype in obesity
    Article Snippet: Non-specific binding in vWF staining was prevented by incubation with mouse Fc-blocking solution (1:200, BD Biosciences) for 1 h. Isotype controls were applied to confirm specificity of primary antibodies. .. Sections were washed and incubated with fluorochrome-conjugated secondary antibodies ( ) for 1 h in the dark, followed by DAPI staining (1:1000 in PBS, Thermo Fisher Scientific) for 15 min. Autofluorescence of elastic fibers was suppressed using the TrueVIEW autofluorescence quenching Kit (Vector Laboratories, Newark, USA). .. Coverslips were mounted onto glass slides with a fluorescence mounting medium (Dako, Agilent, Santa-Clara, USA) and imaged using the BZ-9000 microscope (Keyence, Osaka, Japan).



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    Image Search Results


    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Journal: Biomaterials and Biosystems

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    doi: 10.1016/j.bbiosy.2026.100137

    Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

    Techniques: Staining, Cell Culture