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diamidino 2 phenylindole dapi staining  (10X Genomics)

 
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    10X Genomics diamidino 2 phenylindole dapi staining
    Diamidino 2 Phenylindole Dapi Staining, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/dapi/pm42135486-420-0-17
    Average 86 stars, based on 1 article reviews
    diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Spatial transcriptomic analysis of adult hippocampal neurogenesis in the human brain
    Article Snippet: .. 168 (a) Adult mouse section stained for DAPI and NeuN (https://www.10xgenomics.com/datas- ets/adult-169 mouse-brain-section-1-coronal-stains-dapi-anti-neu-n-1-standard-1-1-0). (b) Elbow plot generated with 170 BayesSpace showing the pseudo-log-likelihood as a function of the number of clusters (q). q=24 was chosen 171 as an appropriate elbow of the plot. (c) Unsupervised clustering of the mouse Visium dataset generated by 172 10x Genomics using the R package BayesSpace (k=24). (d) Log-normalized expression of neurogenesis 173 markers spatially plotted at the sub-spot level using BayesSpace. ..

    Article Title: Unraveling lncRNA diversity at a single cell resolution and in a spatial context across different cancer types.
    Article Snippet: In situ hybridization was carried out overnight using 380 probes from the pre-designed Xenium Immuno Oncology Panel (10x Genomics) and from the add-on custom panel of 76 cuTARs and 19 coding genes not represented in the base panel. .. 4,6-diamidino-2-phenylindole (DAPI) staining was used to label nuclei, which were used for the estimation of cell boundaries (10x Genomics, CG000582, Rev D). ..

    Article Title: Integrating 12 Spatial and Single Cell Technologies to Characterise Tumour Neighbourhoods and Cellular Interactions in three Skin Cancer Types
    Article Snippet: In situ hybridisation was carried out overnight using 260 probes from the pre-designed Xenium Human Skin Panel (10x Genomics). .. DAPI staining was used to label nuclei, which were used for the estimation of cell boundaries (10x Genomics, CG000582, Rev D). ..

    Article Title: Tumour evolution and microenvironment interactions in 2D and 3D space.
    Article Snippet: .. Nuclei were stained with DAPI to improve sample tracking and approximate cell boundaries (10x Genomics, CG000582, Rev D). .. These samples, along with buffers and decoding consumables, were loaded into a Xenium analyzer (10x Genomics, 1000481).

    Article Title: Comparative analysis of BCG vaccination routes in mice reveals preferential reprogramming of pulmonary macrophages upon mucosal administration
    Article Snippet: .. Following sample placement, sections were stained with DAPI according to the protocol CG000312 (10X Genomics) and imaged using a Leica DMI 4000/6000 Widefield Microscope. .. Sections were then permeabilized and prepared for library preparation according to the protocol CG000239 (10X Genomics).

    Generated:

    Article Title: Spatial transcriptomic analysis of adult hippocampal neurogenesis in the human brain
    Article Snippet: .. 168 (a) Adult mouse section stained for DAPI and NeuN (https://www.10xgenomics.com/datas- ets/adult-169 mouse-brain-section-1-coronal-stains-dapi-anti-neu-n-1-standard-1-1-0). (b) Elbow plot generated with 170 BayesSpace showing the pseudo-log-likelihood as a function of the number of clusters (q). q=24 was chosen 171 as an appropriate elbow of the plot. (c) Unsupervised clustering of the mouse Visium dataset generated by 172 10x Genomics using the R package BayesSpace (k=24). (d) Log-normalized expression of neurogenesis 173 markers spatially plotted at the sub-spot level using BayesSpace. ..

    Expressing:

    Article Title: Spatial transcriptomic analysis of adult hippocampal neurogenesis in the human brain
    Article Snippet: .. 168 (a) Adult mouse section stained for DAPI and NeuN (https://www.10xgenomics.com/datas- ets/adult-169 mouse-brain-section-1-coronal-stains-dapi-anti-neu-n-1-standard-1-1-0). (b) Elbow plot generated with 170 BayesSpace showing the pseudo-log-likelihood as a function of the number of clusters (q). q=24 was chosen 171 as an appropriate elbow of the plot. (c) Unsupervised clustering of the mouse Visium dataset generated by 172 10x Genomics using the R package BayesSpace (k=24). (d) Log-normalized expression of neurogenesis 173 markers spatially plotted at the sub-spot level using BayesSpace. ..

    other:

    Article Title: CellBinDB: a large-scale multimodal annotated dataset for cell segmentation with benchmarking of universal models
    Article Snippet: DAPI human skin melanoma: https://www.10xgenomics.com/datasets/human-melanoma-if-stained-ffpe-2-standard .

    Microscopy:

    Article Title: Comparative analysis of BCG vaccination routes in mice reveals preferential reprogramming of pulmonary macrophages upon mucosal administration
    Article Snippet: .. Following sample placement, sections were stained with DAPI according to the protocol CG000312 (10X Genomics) and imaged using a Leica DMI 4000/6000 Widefield Microscope. .. Sections were then permeabilized and prepared for library preparation according to the protocol CG000239 (10X Genomics).



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    Image Search Results


    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Journal: Biomaterials and Biosystems

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    doi: 10.1016/j.bbiosy.2026.100137

    Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

    Techniques: Staining, Cell Culture