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Sangon Biotech dapi stain solution
Dapi Stain Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/dapi+solution+staining/pmc13138055-43-0-4
Average 86 stars, based on 1 article reviews
dapi stain solution - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: Enterohemorrhagic Escherichia coli senses colonic oxygen availability to modulate its virulence and colonization
Article Snippet: .. DAPI Stain Solution , Sangon Biotech , Cat#E607303. .. Actin-Tracker Green-488 , Beyotime , Cat#C2221S.

Article Title: An Immune Microenvironment-Integrated Intestinal-on-a-Chip Model for Investigating Immunopathogenesis in Inflammatory Bowel Disease.
Article Snippet: .. Finally, add DAPI staining solution (Sangon Biotech, Shanghai, China) and incubate in the dark for 5 min to stain cell nuclei. ..

Article Title: Deciphering OCT4A-dose-dependent transcriptional profiles associated with tumorigenic potential in somatic cancer cells.
Article Snippet: Thereafter, cells were incubated with anti-OCT4A (Cell Signaling Technology 2890S) at 4°C overnight. .. Nuclei were counterstained with DAPI Stain Solution (Sangon Biotech E607303-0020). .. Images were captured using a ZEISS Axiovert 5 Microscope.

Article Title: The calcium ion channel protein TRPML is indispensable to the reproduction of Nilaparvata lugens and the transovarial transmission of its yeast-like symbionts.
Article Snippet: The secondary antibody was a goat anti-rabbit IgG antibody conjugated with Dylight 488 fluorescent dye (Abbkine, Atlanta, GA, USA). .. Nuclei were stained with DAPI staining solution (Sangon Biotech, Shanghai, China). .. Samples were observed using a laser scanning confocal microscope (SP8; Leica, Mannheim, Germany).

Article Title: Harnessing the yeast nucleus for the efficient production of valuable monoterpenes.
Article Snippet: Subcellular engineering emerges as a pivotal strategy to amplify terpene production in yeast cell factories.. The yeast nucleus, which shares acetyl-CoA pool with the cytosol and maintains nuclear membrane integrity during mitosis, offers unique compartmentalization capabilities.. Despite these attributes, there is a notable absence of research harnessing the potential of the yeast nucleus for subcellular engineering.

Article Title: An Immune Microenvironment-Integrated Intestinal-on-a-Chip Model for Investigating Immunopathogenesis in Inflammatory Bowel Disease
Article Snippet: .. Finally, add DAPI staining solution (Sangon Biotech, Shanghai, China) and incubate in the dark for 5 min to stain cell nuclei. ..

Article Title: TRIM47 Regulates Energy Metabolism via Glycolytic Reprogramming to Drive Hepatocellular Carcinoma Progression and Represents an Efficient Therapeutic Target
Article Snippet: Meanwhile, experimental cells were seeded in confocal dishes (Thermo Fisher Scientific, USA) at a density of 20%–30% and cultured overnight in a sugar‐free medium. .. The cells were subsequently incubated in glucose‐free DMEM containing 20 μ m 2‐NBDG (Thermo Fisher Scientific, USA) for 2 h. After being washed with PBS, the cells were fixed with 4% paraformaldehyde, and a DAPI working solution (Sangon Biotech, China) was added to stain the cell nuclei for 5 min. .. The stained sections were visualized via a confocal microscope (Zeiss, Germany).

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.

Incubation:

Article Title: TRIM47 Regulates Energy Metabolism via Glycolytic Reprogramming to Drive Hepatocellular Carcinoma Progression and Represents an Efficient Therapeutic Target
Article Snippet: Meanwhile, experimental cells were seeded in confocal dishes (Thermo Fisher Scientific, USA) at a density of 20%–30% and cultured overnight in a sugar‐free medium. .. The cells were subsequently incubated in glucose‐free DMEM containing 20 μ m 2‐NBDG (Thermo Fisher Scientific, USA) for 2 h. After being washed with PBS, the cells were fixed with 4% paraformaldehyde, and a DAPI working solution (Sangon Biotech, China) was added to stain the cell nuclei for 5 min. .. The stained sections were visualized via a confocal microscope (Zeiss, Germany).

Polymerase Chain Reaction:

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.

Plasmid Preparation:

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.

DNA Extraction:

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.



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Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
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Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
Dapi Stain Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining/dapi+solution+staining/pmc13138055-43-0-4
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Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. <t>Phalloidin/DAPI</t> staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.
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Image Search Results


Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Journal: Biomaterials and Biosystems

Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

doi: 10.1016/j.bbiosy.2026.100137

Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

Techniques: Staining, Cell Culture