Journal: PPAR Research
Article Title: PPAR γ Agonist Rosiglitazone Suppresses Renal mPGES-1/PGE2 Pathway in db/db Mice
doi: 10.1155/2013/612971
Figure Lengend Snippet: Effect of Rosi treatment on the protein expressions of mPGES-2, cPGES, and 15-PGDH in kidneys of db/db mice. (a) Western blots of mPGES-2, cPGES, 15-PGDH, and β -actin. (b) Densitometry of western blots. N = 4-5 in each group. Data are mean ± SE.
Article Snippet: The blots were blocked overnight with 5% nonfat dry milk in Tris-buffered saline (TBS), followed by incubation for 1 h with rabbit anti-COX-1 (Cayman Chemicals, Cat no. 160108), anti-COX-2 (Cayman Chemicals, Cat no. 160106), anti-mPGES-1 (Cayman Chemicals, Cat no. 160140), anti-mPGES-2 (Cayman Chemicals, Cat no. 160145), anti-cPGES (Cayman Chemicals, Cat no. 160150), anti-15-PGDH (Cayman Chemicals, Cat no. 160615), anti-PPAR γ (Santa Cruz, CAT no. SC-7273), and anti β -actin (Sigma Aldrich, Cat no. A1978) at a dilution of 1 : 1000.
Techniques: Western Blot