col2a1 (Servicebio Inc)
86
Structured Review
Servicebio Inc
col2a1
Col2a1, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col2a1/anti+col2a1/pmc13265696-99-12-13
Average 86 stars, based on 1 article reviews
Col2a1, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col2a1/anti+col2a1/pmc13265696-99-12-13
Average 86 stars, based on 1 article reviews
col2a1 - by Bioz Stars,
2026-09
86/100 stars
Images
1) Product Images from "Dermal fibroblasts attenuate osteoarthritis by restoring synovial fibroblast homeostasis"
Article Title: Dermal fibroblasts attenuate osteoarthritis by restoring synovial fibroblast homeostasis
Journal: Journal of Orthopaedic Translation
doi: 10.1016/j.jot.2026.101138
Figure Legend Snippet: Intra-articular injection of DFbs preserved articular cartilage structure and homeostasis. A) H&E staining and SO/FG staining of articular cartilage. B) Representative micro-CT 3D images and coronal images of knee joints of rats at 8 weeks after ACLT. C) Grading of osteoarthritic cartilage damage (OARSI Criteria). D) Immunofluorescence staining of MMP3, MMP13, iNOS and COL2A1. E) Quantification of positive cells and positive area in the cartilage. n = 6. Data were presented as mean ± SD. Results were analyzed by one-way ANOVA.
Techniques Used: Injection, Staining, Micro-CT, Immunofluorescence
Figure Legend Snippet: DFbs improve the inflammatory phenotype of synovial fibroblasts and chondrocytes via paracrine secretion. A) Schematic of the transwell co-culture system for synovial fibroblasts/chondrocytes and DFbs. B) Volcano plot of DEGs in the FI group and DFI group. C) GO enrichment analysis of DEGs between the FI group and DFI group. D) GSEA highlighting the enrichment of gene sets associated with inflammatory response. E) Expression profiles of inflammation-related genes and cell adhesion-related genes in the three cell groups. F) IL-6 immunofluorescence staining of synovial fibroblasts in the normal culture, IL-1β-stimulated, and IL-1β + DFb co-culture groups. Scale bar: 50 μm (Row 1); 25 μm (Row 2). G) Quantification of IL-6+ cells. n = 3. Data were presented as mean ± SD. Results were analyzed by one-way ANOVA. H) Axial fluorescence intensity of IL-6 in representative synovial fibroblasts. I) Volcano plot of DEGs between the CI group and DCI group. J) Intersection of downregulated genes in IL1β-stimulated chondrocytes and upregulated genes in IL1β-stimulated chondrocytes co-cultured with DFbs (vs. IL1β-stimulated chondrocytes alone) and intersection of upregulated genes in IL1β-stimulated chondrocytes and downregulated genes in IL1β-stimulated chondrocytes co-cultured with DFbs (vs. IL1β-stimulated chondrocytes alone). K) GO enrichment analysis of genes derived from the aforementioned intersection. L) Clustered heatmap of genes related to inflammation and ECM. M) RT-qPCR analysis of Mmp9 , Mmp13 , Acan , and Col2a1 . n = 6. Data were presented as mean ± SD. Results were analyzed by one-way ANOVA. N) Alcian blue staining of chondrocytes in three groups (Control, IL-1β-stimulated group, IL-1β-stimulated + DFb co-culture group). Scale bar: 2 mm.
Techniques Used: Co-Culture Assay, Expressing, Immunofluorescence, Staining, Fluorescence, Cell Culture, Derivative Assay, Quantitative RT-PCR, Control
Figure Legend Snippet: Paracrine APOD from DFbs inhibited inflammatory activation of FLS and catabolic phenotype of chondrocytes. A) Workflow diagram of proteomic sequencing of fibroblast supernatants. B) Intersection of proteins detected in DFb supernatant, human secreted proteins, and human secreted immunoglobulins. C) GO enrichment analysis of secreted proteins from DFbs. D) Intersection of DFb secreted proteins, downregulated DEGs in OA SL Fb, and downregulated DEGs in in vitro cultured OA FLS. E) Cluster heatmap of expression profiles of genes from the aforementioned intersection in DFbs, healthy synovial fibroblasts (HF), and OA FLS. F) Fold change in APOD mRNA expression between healthy SL Fb and OA SL Fb (with healthy SL Fb as the reference). G) Fold change in APOD mRNA expression between healthy synovial fibroblasts (HF), DFb and OA FLS (with healthy synovial fibroblasts as the reference). H, J) RT-qPCR analysis of pro-inflammatory genes ( Nos2 , Il6 , Ptgs2 ) in FLS (H), and matrix metabolic genes ( Mmp9 , Mmp13 , Col2a1 ) in chondrocytes (J), after co-culture with control, empty vector-transfected, APOD-overexpressing (APOD-OE) DFbs, or treatment with recombinant APOD protein. I, K) RT-qPCR analysis of the above gene panels in FLS (I) and chondrocytes (K), after co-culture with control, negative control siRNA-transfected, or APOD-knockdown (APOD-si) DFb. n = 6. Data were presented as mean ± SD. Results were analyzed by one-way ANOVA. ∗∗P < 0.01. L) Representative Western blots of iNOS and IL-6 in FLS from the indicated groups, with β-actin (ACTB) as the loading control. M) Representative Western blots of MMP9 in chondrocytes from the indicated groups, with ACTB as the loading control.
Techniques Used: Activation Assay, Sequencing, In Vitro, Cell Culture, Expressing, Quantitative RT-PCR, Co-Culture Assay, Control, Plasmid Preparation, Transfection, Recombinant, Negative Control, Knockdown, Western Blot
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