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cisplatin  (MedChemExpress)


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    Structured Review

    MedChemExpress cisplatin
    Cisplatin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cisplatin/Cisplatin/custom%40hy-17394%4042664821
    Average 99 stars, based on 1108 article reviews
    cisplatin - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Solvent:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod

    Recombinant:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod

    Cell Culture:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod

    Concentration Assay:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod

    Gene Expression:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod

    Suspension:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod

    Expressing:

    Article Title: PDK1/AKT signaling is involved in the anti-tumor effect of gramine and cisplatin chemoresistance in ovarian cancer cells
    Article Snippet: ˚C in a humidified atmosphere containing 5% CO 2 . Gramine (HY-N0166, purity: 99.80%), Cisplatin (HY-17394, purity: 99.30%) and dimethyl sulfoxide (DMSO; HY-Y0320C, purity: 99.98%) were purchased from MedChemExpress. DMSO was used as the vehicle, and the final concentration of DMSO in all cell culture systems was maintained <0.1% (v/v) to exclude solvent cytotoxicity. Recombinant lentiviruses encod



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    Detection of ferroptosis‐related pathway in chemoresistant NSCLC cells. (A) Detection of IC 50 in A549 and <t>A549/DDP</t> cell lines. (B) Western blot analysis of the expression levels of SLC7A11, GPX4, and FTH1, in A549 and A549/DDP. (C) qRT‐PCR for SLC7A11, GPX4, and FTH1 mRNA levels in A549 and A549/DDP. (D) Flow cytometry analysis of Lipid ROS levels in A549 cells and A549/DDP. (E) Verification of Lipid ROS fluorescence intensity in A549 and A549/DDP. (F) Detection of GSH levels in A549 and A549/DDP. (G) Detection of MDA levels in A549 and A549/DDP. (H) CCK‐8 assay for the sensitivity of A549/DDP cells to <t>cisplatin</t> after Erastin treatment. (ns, no significant, * p < 0.05, ** p < 0.01, *** p < 0.001).
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    Image Search Results


    Detection of ferroptosis‐related pathway in chemoresistant NSCLC cells. (A) Detection of IC 50 in A549 and A549/DDP cell lines. (B) Western blot analysis of the expression levels of SLC7A11, GPX4, and FTH1, in A549 and A549/DDP. (C) qRT‐PCR for SLC7A11, GPX4, and FTH1 mRNA levels in A549 and A549/DDP. (D) Flow cytometry analysis of Lipid ROS levels in A549 cells and A549/DDP. (E) Verification of Lipid ROS fluorescence intensity in A549 and A549/DDP. (F) Detection of GSH levels in A549 and A549/DDP. (G) Detection of MDA levels in A549 and A549/DDP. (H) CCK‐8 assay for the sensitivity of A549/DDP cells to cisplatin after Erastin treatment. (ns, no significant, * p < 0.05, ** p < 0.01, *** p < 0.001).

    Journal: The FASEB Journal

    Article Title: PCK2 Inhibition Reverses Cisplatin Resistance of Non‐Small Cell Lung Cancer by Triggering Ferroptosis

    doi: 10.1096/fj.202602464R

    Figure Lengend Snippet: Detection of ferroptosis‐related pathway in chemoresistant NSCLC cells. (A) Detection of IC 50 in A549 and A549/DDP cell lines. (B) Western blot analysis of the expression levels of SLC7A11, GPX4, and FTH1, in A549 and A549/DDP. (C) qRT‐PCR for SLC7A11, GPX4, and FTH1 mRNA levels in A549 and A549/DDP. (D) Flow cytometry analysis of Lipid ROS levels in A549 cells and A549/DDP. (E) Verification of Lipid ROS fluorescence intensity in A549 and A549/DDP. (F) Detection of GSH levels in A549 and A549/DDP. (G) Detection of MDA levels in A549 and A549/DDP. (H) CCK‐8 assay for the sensitivity of A549/DDP cells to cisplatin after Erastin treatment. (ns, no significant, * p < 0.05, ** p < 0.01, *** p < 0.001).

    Article Snippet: Cisplatin (DDP), Necrostatin‐1, Z‐VAD‐FMK, Ferrostatin‐1, and Chloroquine were purchased from MedChemexpress (USA).

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Flow Cytometry, Fluorescence, CCK-8 Assay