Journal: Assay and drug development technologies

Article Title: Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.

doi: 10.1089/adt.2020.1020

Figure Lengend Snippet: Fig. 8. Cisplatin dosing on carcinoma of MCF-7 breast cancer cells grown in a standard 96-well plate. (a) Cancer cell viability responses at varying cisplatin dosing. Relative standard deviation of 5.0% (n = 4). The cumulative distribution plot was used to calculate the inhibitory dosing effect that reduced the cell viability by at least 50% (b) directly in a 96-well plate. Composite scaffolds of: (c) SNP:PEG, (d) SNP:PEG:KPFE, and (e) SNP:PEG:KPLM. The cumulative distribution was obtained by adding the successive cell viability (%) values. Subsequently, the 50% of the cumulative distri- bution was used for the calculation of IC50. The cancer cells were plated at 10,000 cells using 100 mL volume, with 200 mL of cisplatin concentration added to each test samples.

Article Snippet: Cisplatin was purchased from Tocris Bioscience (Minneapolis, MN) and supplied by Invitrogen,.

Techniques: Standard Deviation, Concentration Assay

Journal: Cell reports

Article Title: Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis

doi: 10.1016/j.celrep.2020.02.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Single cell suspensions were stained with Cell-ID Cisplatin (Fluidigm), blocked with Fc Receptor Binding Inhibitor (Thermo Fisher), and stained with heavy metal-conjugated antibodies (Fluidigm) for surface staining.

Techniques: Recombinant, Staining, cDNA Synthesis, SYBR Green Assay, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Software

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Chemotherapeutic Drugs Alter Functional Properties and Proteome of Mouse Testicular Germ Cells In Vitro.

doi: 10.1093/toxsci/kfy098

Figure Lengend Snippet: Figure 2. In vitro proliferation of testicular germ cells after drugs’ exposure. (A) Fluorescent microscopic image of in vitro proliferated testicular germ cell of GFP mouse treated with etoposide, cisplatin, bleomycin,

Article Snippet: Chemotherapeutic agents or drugs named as bleomycin sulfate, etoposide and cisplatin were collected from LKT Laboratories (St Paul, Minnesota).

Techniques: In Vitro

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Chemotherapeutic Drugs Alter Functional Properties and Proteome of Mouse Testicular Germ Cells In Vitro.

doi: 10.1093/toxsci/kfy098

Figure Lengend Snippet: Figure 3. Effects of chemotherapeutic drugs on the apoptotic rate of testicular germ cells. (A) Flow cytometric determination of the apoptotic rate of drugs treated cultured germ cells after stained with Annexin V/PI. Bar graphs (B), (C), (D), and (E) represent the percentages of apoptotic germ cells due to administration of etoposide, cisplatin, bleomycin, and BEP respectively. Values are represented mean ± SEM of 6 independent experiments. Different letters (a and b) indicate significant difference (p < 0.05) compared

Article Snippet: Chemotherapeutic agents or drugs named as bleomycin sulfate, etoposide and cisplatin were collected from LKT Laboratories (St Paul, Minnesota).

Techniques: Cell Culture, Staining

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Chemotherapeutic Drugs Alter Functional Properties and Proteome of Mouse Testicular Germ Cells In Vitro.

doi: 10.1093/toxsci/kfy098

Figure Lengend Snippet: Figure 5. Effects of chemotherapeutic agents on the stemness properties of spermatogonial stem cells (SSCs). Stemness properties of SSCs were evaluated by counting donor germ cell derived colonies from recipient testes. Cultured germ cells treated with 0.05 µM etoposide, 1 µM cisplatin, 10 µM bleomycin, and 0.1 µM BEP were transplanted and colonies were counted after 2 months 5of transplantation. (A) Testes of

Article Snippet: Chemotherapeutic agents or drugs named as bleomycin sulfate, etoposide and cisplatin were collected from LKT Laboratories (St Paul, Minnesota).

Techniques: Derivative Assay, Cell Culture, Transplantation Assay

Journal: Oncology reports

Article Title: SBI0206965, a novel inhibitor of Ulk1, suppresses non-small cell lung cancer cell growth by modulating both autophagy and apoptosis pathways.

doi: 10.3892/or.2017.5635

Figure Lengend Snippet: Figure 3. Knockdown of Ulk1 sensitizes NSCLC cells to cisplatin-induced apoptosis and inhibition of cell proliferation. (A) The relative mRNA expression of Ulk1 after cisplatin treatment for 36 h in A549 and H460 cells. (B) Western blot analysis of changes in Ulk1 levels after cisplatin treatment for 48 h in A549 and H460 cells. (C) The percentages of apoptotic cells with Annexin V staining were determined by flow cytometry after transfection with the indicated shRNA plasmids for 48 h and treatment with the indicated concentrations of cisplatin for 48 h. (D and E) The proliferation of A549 and H460 cells was determined by CCK8 assay after transfection and cisplatin treatment. **P<0.01, t-test.

Article Snippet: Cisplatin and Z-VAD-FMK were purchased from Selleck (Houston, TX, USA).

Techniques: Knockdown, Inhibition, Expressing, Western Blot, Staining, Flow Cytometry, Transfection, shRNA, CCK-8 Assay

Journal: Oncology reports

Article Title: SBI0206965, a novel inhibitor of Ulk1, suppresses non-small cell lung cancer cell growth by modulating both autophagy and apoptosis pathways.

doi: 10.3892/or.2017.5635

Figure Lengend Snippet: Figure 5. SBI0206965 sensitizes NSCLC cells to cisplatin. (A and B) Viability of NSCLC cells after treating with the specified doses of cisplatin and SBI0206965 for 72 h. (C and D) After 24 h of cisplatin and SBI0206965 exposure, the apoptosis of A549 and H460 cells was analyzed by flow cytometry as described in Materials and methods. **P<0.01, t-test.

Article Snippet: Cisplatin and Z-VAD-FMK were purchased from Selleck (Houston, TX, USA).

Techniques: Flow Cytometry

Journal: Oncology reports

Article Title: SBI0206965, a novel inhibitor of Ulk1, suppresses non-small cell lung cancer cell growth by modulating both autophagy and apoptosis pathways.

doi: 10.3892/or.2017.5635

Figure Lengend Snippet: Figure 6. SBI0206965 sensitizes NSCLC cells to cisplatin partly through autophagy inhibition. (A-C) Western blot analysis of changes in LC3 I, LC3 II, and p62 levels after SBI0206965 and/or cisplatin treatment. (D) A549 cells were transfected with GFP-LC3 plasmids, and the cells were then treated with SBI0206965 and cisplatin either alone or in combination for 24 h. GFP- LC3 dots were observed by laser confocal microscopy. The presented results are representative of three independent experiments. Scale bars, 10 µm.

Article Snippet: Cisplatin and Z-VAD-FMK were purchased from Selleck (Houston, TX, USA).

Techniques: Inhibition, Western Blot, Transfection, Confocal Microscopy

Journal: Molecular medicine reports

Article Title: Cisplatin in combination with programmed cell death protein 5 increases antitumor activity in prostate cancer cells by promoting apoptosis.

doi: 10.3892/mmr.2015.3252

Figure Lengend Snippet: Figure 1. MTT assays determine the survival rates of Du145 prostate cancer cells following cisplatin treatment and Pdcd5 transfection. (A) Chemical structure of cisplatin. (B) Survival rate of Du145 cells following treatment with various concentrations of cisplatin (0, 10, 50 and 250 µM) for 24, 48 and 72 h. (C) Cell viability of Du145 cells transfected with the Pdcd5 or mock plasmid vectors following cisplatin treatment for 24, 48 and 72 h. Values are presented as the mean ± standard deviation (n=3). *P<0.05 vs. untreated cells, **P<0.01 vs. untreated cells. Pdcd5, programmed cell death protein 5; mock, cells transfected with an empty vector.

Article Snippet: Fol lowing t ransfection, cisplatin-treated Du145 cells were subjected to Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual labeling followed by fluorescence‐activated cell sorting (FACS) analysis (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), performed according to the manufacturer's instruction, in order to determine apoptosis.

Techniques: Transfection, Plasmid Preparation, Standard Deviation

Journal: Molecular medicine reports

Article Title: Cisplatin in combination with programmed cell death protein 5 increases antitumor activity in prostate cancer cells by promoting apoptosis.

doi: 10.3892/mmr.2015.3252

Figure Lengend Snippet: Figure 2. Effect of Pdcd5 overexpression on the chemosensitivity of Du145 prostate cancer cells. (A) Morphological changes of Du145 following transient transfection of a Pdcd5 or mock vector (magnification, x200). (B) An MTT assay was used to detect cell viability of mock‑ and Pdcd5‑transfected Du145 cells treated with various concentrations of cisplatin (0, 10, 50 and 250 µM) for 24, 48 and 72 h. Values are presented as the mean ± standard deviation (n=3). *P<0.05 vs. mock vector, **P<0.01 vs. mock vector. Pdcd5, programmed cell death protein 5.

Article Snippet: Fol lowing t ransfection, cisplatin-treated Du145 cells were subjected to Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual labeling followed by fluorescence‐activated cell sorting (FACS) analysis (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), performed according to the manufacturer's instruction, in order to determine apoptosis.

Techniques: Over Expression, Transfection, Plasmid Preparation, MTT Assay, Standard Deviation

Journal: Molecular medicine reports

Article Title: Cisplatin in combination with programmed cell death protein 5 increases antitumor activity in prostate cancer cells by promoting apoptosis.

doi: 10.3892/mmr.2015.3252

Figure Lengend Snippet: Figure 3. Cell viability of Du145 and PC3 prostate cancer cells following transfection of Pdcd5. MTT assays were used to determine the IC50 values of (A) Du145 and (B) PC3 cells following treatment with various concentrations of cisplatin (1, 5, 10, 25, 50, 100, 250 and 500 µM) for 48 h. Values are presented as the mean ± standard deviation (n=3). Pdcd5, programmed cell death protein 5.

Article Snippet: Fol lowing t ransfection, cisplatin-treated Du145 cells were subjected to Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual labeling followed by fluorescence‐activated cell sorting (FACS) analysis (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), performed according to the manufacturer's instruction, in order to determine apoptosis.

Techniques: Transfection, Standard Deviation

Journal: Molecular medicine reports

Article Title: Cisplatin in combination with programmed cell death protein 5 increases antitumor activity in prostate cancer cells by promoting apoptosis.

doi: 10.3892/mmr.2015.3252

Figure Lengend Snippet: Figure 5. Western blot analysis of apoptosis‑associated protein expression levels in Pdcd5‑transfected Du145 prostate cancer cells following treatment with 50 µM cisplatin. Du145 cells were treated with 50 µM cisplatin for 24 h. β‑actin was used as an internal control. Ctrl, control group of untransfected Du145 cells.

Article Snippet: Fol lowing t ransfection, cisplatin-treated Du145 cells were subjected to Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual labeling followed by fluorescence‐activated cell sorting (FACS) analysis (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), performed according to the manufacturer's instruction, in order to determine apoptosis.

Techniques: Western Blot, Expressing, Control

Journal: Molecular medicine reports

Article Title: Cisplatin in combination with programmed cell death protein 5 increases antitumor activity in prostate cancer cells by promoting apoptosis.

doi: 10.3892/mmr.2015.3252

Figure Lengend Snippet: Figure 4. Annexin V‑FITC/PI staining and fluorescence‑activated cell sorting analysis of apotosis in Du145 cells following transfection of Pdcd5 and cis platin treatment. (A) Analysis of apoptosis in Du145 cells transfected with Pdcd5 or mock plasmind vectors with or without cisplatin treatment for 24 h. (B) Histogram of apoptotic rates of Du145 cells in different treatment groups. Values are presented as the mean ± standard deviation (n=3). Pdcd5, programmed cell death protein 5; FITC, fluorescein isothiocyanate; PI, propidium iodide.

Article Snippet: Fol lowing t ransfection, cisplatin-treated Du145 cells were subjected to Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) dual labeling followed by fluorescence‐activated cell sorting (FACS) analysis (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), performed according to the manufacturer's instruction, in order to determine apoptosis.

Techniques: Staining, FACS, Transfection, Standard Deviation