cidec  (MedChemExpress)

Journal: The Journal of Biological Chemistry

Article Title: Blockade of the estrogen receptor alpha–pregnane X receptor axis protects ovariectomized mice against ethanol-induced hepatotoxicity

doi: 10.1016/j.jbc.2025.110238

Figure Lengend Snippet: Hepatic gene expression and immunoblot analysis of lipogenic enzymes. Sham and OVX mice were pair-fed either a 5% ethanol (EtOH)-containing liquid diet or control diet for 10 days followed by a single oral dose of EtOH (5 g/kg, 30% EtOH solution) on day 11, and mRNA and liver homogenate were prepared for the assays as described in the Experimental procedures section. A , Liver Srebp1 , Cd36 , Scd1 , Scd2 , Fas , and Acc1a mRNAs (n = 5–6). B , Liver Pparγ mRNA and ( C ) liver Fsp27 / Cidec mRNA (n = 6). D , Liver PPARγ protein, E , liver FSP27/CIDEC protein, F , liver FAS protein, and ( G ) liver ACC1α protein (n = 3–4). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 between indicated groups. OVX, ovariectomy; PPARγ, peroxisome proliferator–activated receptor gamma.

Article Snippet: Fsp27/Cidec , ThermoFisher , Mm00617672_m1.

Techniques: Gene Expression, Western Blot, Control

Journal: Cell Death and Differentiation

Article Title: Epigenetic regulation of HOXA2 expression affects tumor progression and predicts breast cancer patient survival

doi: 10.1038/s41418-024-01430-2

Figure Lengend Snippet: ( a ) Bubble plots showing the significant positive correlation values (R∈[0;1], p-value ≤ 0.05) between the expression of the three selected genes ( HOXA2 , CIDEC and PPARγ) in breast non-tumor/normal and cancer tissues from our cohort (left graph) and from TCGA dataset (right graph). Bubble color (from light to dark blue) indicates the strength (R value) of the correlation. Of note, a strong correlation is in dark blue and corresponds to a R = 1, while a weak correlation is in light blue and corresponds to a R = 0. Bubble size indicates the significance (p-value) of the correlation; bigger is the size of the bubble, lower is the p-value. ( b, c ) The mRNA expression of CIDEC ( b ) and of PPARγ ( c ) was measured in hTERT-HME1 cells wild type (WT) or knockout ( HOXA KO ) for HOXA2 by RT-qPCR. GAPDH was used as endogenous control. ( d, e ) Western blot analysis of the expression of CIDEC and PPARγ in WT and HOXA2 KO cells ( d ) and in WT and HOXA2 KO cells transfected with HOXA2 plasmid (pHOXA2) or the control vector (pCMV) ( e ). Antibodies for Flag ( e ) or β-actin ( d, e ) were employed as control of overexpression of HOXA2 or as loading control, respectively. Experimental workflow employed for lipid droplet tracing ( f ) ( f ; created with BioRender.com). Confocal microscopy images (scale bar equals 10 μm) ( g ) and quantification of the number ( h ) of intracellular lipid droplets (LDs) normalized to cell area and expressed as fold change (FC) to control in hTERT-HME1 WT and HOXA2 KO cells with (+pHOXA2) or without (−pHOXA2) restoration of HOXA2 expression. LDs were stained with BODIPY 493/503 (green), and nuclei were stained with Hoechst (blue). b – e , h Data are presented as mean of ± SD from one representative experiment and analyzed with Student’s t -test or ANOVA. FC fold change; hTERT_WT hTERT-HME1 wild type cells; hTERT_HOXA2 KO hTERT-HME1 HOXA2 knockout cells; RT-qPCR reverse-transcription quantitative real time PCR.

Article Snippet: Then, 100 ng of cDNA were amplified with specific Taqman probes ( HOXA2: Hs00534579_m1, PPARG: Hs01115513_m1, CIDEC : Hs01032998_m1, all from Thermo Fisher Scientific) carrying out the qPCR on the StepOne TM Real-time PCR system (Thermo Fisher Scientific) using TaqMan Fast Advanced Master Mix (Applied Biosystems TM ), according to the manufacturer’s instructions.

Techniques: Expressing, Knock-Out, Quantitative RT-PCR, Control, Western Blot, Transfection, Plasmid Preparation, Over Expression, Confocal Microscopy, Staining, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Human PXR Signaling Exacerbates Ethanol-induced Liver Injury in Females: Evidence From Mouse Models and Human Alcohol-associated Liver Disease Cohorts

doi: 10.1016/j.jcmgh.2025.101589

Figure Lengend Snippet: Plots for selected genes from microarray gene expression. Total RNA extracted from frozen liver was evaluated for changes using microarray. ( A ) Adh4; ( B ) Aldh4a1; ( C ) Cyp2b10 ; ( D ) Cidec ; ( E ) Ppargc1a ; ( F ) Apoa4 ; ( G ) Tnfrsf12a ; ( H ) Cyp2b9 ; ( I ) Cyp2b13 ; ( J ) Sult2a1 ; ( K ) Sult2a2 ; and ( L ) Acot2 . Data represents mean ± SEM (n = 6). ∗ P ≤ .05, ∗∗ P ≤ .01, ∗∗∗ P ≤ .001, ∗∗∗∗ P ≤ .0001 between indicated groups.

Article Snippet: CIDEC , Hs00535724_gH.

Techniques: Microarray, Gene Expression

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Human PXR Signaling Exacerbates Ethanol-induced Liver Injury in Females: Evidence From Mouse Models and Human Alcohol-associated Liver Disease Cohorts

doi: 10.1016/j.jcmgh.2025.101589

Figure Lengend Snippet: Effect of ethanol on alcohol metabolism and PXR target genes. Total RNA was extracted from frozen liver obtained and evaluated for changes in mRNA expression of the following genes by RT-qPCR relative to the housekeeping Gapdh mRNA : ( A ) Adh1 ; ( B ) Aldh2 ; ( C ) Aldh1b1 ; ( D ) Aldh1a1 ; ( E ) Cat ; ( F ) m Pxr ; ( G ) h PXR ; ( H ) Cyp3a11 ; ( I ) Pparg ; ( J ) Cidec ; ( K ) Fsp27b ; and ( L ) Cd36 . Frozen liver homogenates were evaluated for changes in expression of the following proteins by Western blot relative to the housekeeping protein α-tubulin: ( M ) ADH protein for WT; ( N ) ADH protein for hPXR; ( O ) ALDH2 protein for WT; ( P ) ALDH2 protein for hPXR; ( Q ) ALDH1B1 protein for WT; ( R ) ALDH1B1 protein for hPXR; ( S ) PXR protein for WT; ( T ) PXR protein for hPXR; ( U ) CYP3A protein for WT; and ( V ) CYP3A protein for hPXR. The membranes used to probe for ADH1 ( M ), PXR ( S ), and CYP3A ( U ) protein expression were stripped and reprobed with primary antibodies against ALDH2 ( O ), ALDH1B1 ( Q ) and PXR ( T ). Data represents mean ± SEM (n = 6). ∗ P ≤ .05, ∗∗ P ≤ .01, ∗∗∗ P ≤ .001, ∗∗∗∗ P ≤ .0001 between indicated groups.

Article Snippet: CIDEC , Hs00535724_gH.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Human PXR Signaling Exacerbates Ethanol-induced Liver Injury in Females: Evidence From Mouse Models and Human Alcohol-associated Liver Disease Cohorts

doi: 10.1016/j.jcmgh.2025.101589

Figure Lengend Snippet: Patients with ALD also presented with upregulated lipogenic, inflammatory, and hepatotoxic genes. Total RNA was extracted from liver obtained from ALD and normal patients evaluated for changes in mRNA expression of the following genes by RT-qPCR relative to the housekeeping Gapdh mRNA : ( A ) PXR ; ( B ) CYP2B6 ; ( C ) CIDEC ; ( D ) AKR1B10 ; ( E ) FGF21 ; and ( F ) TNFRSF12A . TMA from liver also presented these results: ( G ) PXR1; ( H ) CYP2B6. Data represents mean ± SEM (n = 4–12). ∗ P ≤ .05, ∗∗ P ≤ .01, ∗∗∗ P ≤ .001, ∗∗∗∗ P ≤ .0001 between indicated groups.

Article Snippet: CIDEC , Hs00535724_gH.

Techniques: Expressing, Quantitative RT-PCR

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Human PXR Signaling Exacerbates Ethanol-induced Liver Injury in Females: Evidence From Mouse Models and Human Alcohol-associated Liver Disease Cohorts

doi: 10.1016/j.jcmgh.2025.101589

Figure Lengend Snippet: No significant changes in gene expression were observed between patients with ALD steatohepatitis and patients with ALD cirrhosis. Total RNA was extracted from liver obtained from ALD and normal patients evaluated for changes in mRNA expression of the following genes by RT-qPCR relative to the housekeeping Gapdh mRNA: ( A ) PXR ; ( B ) CYP2B6 ; ( C ) CIDEC ; ( D ) AKR1B10 ; ( E ) FGF21 ; ( F ) TNFRSF12A . Data represents mean ± SEM (n = 2–11). ∗ P ≤ .05, ∗∗ P ≤ .01, ∗∗∗ P ≤ .001, ∗∗∗∗ P ≤ .0001 between indicated groups.

Article Snippet: CIDEC , Hs00535724_gH.

Techniques: Gene Expression, Expressing, Quantitative RT-PCR