Journal: Oncology Reports
Article Title: LRRC59 inhibits perk pathway-induced apoptosis and promotes cell proliferation, migration and invasion in colorectal cancer cells
doi: 10.3892/or.2025.9010
Figure Lengend Snippet: LRRC59 regulates the PERK-mediated ERS signaling pathway. (A) According to the starBase database, LRRC59 is associated with ERS pathway key genes GRP78, ATF4, and CHOP in colorectal cancer. (B-D) Western blot analysis of PERK pathway-related proteins (GRP78, p-PERK, PERK, ATF4, p-EIF2α, EIF2α and CHOP). *P<0.05, **P<0.01 and ***P<0.001. ER, endoplasmic reticulum; PERK, protein kinase RNA-like ER kinase; ERS, ER stress; p-, phosphorylated; si-, small interfering; NC, negative control; COAD, colon adenocarcinoma.
Article Snippet: The following primary antibodies were used: β-tubulin (1:10,000; cat. no. AP0064; Bioworld Technology, Inc.), LRRC59 (1:10,000; cat. no. 27208-1-AP; Proteintech Group, Inc.), GRP78 (1:10,000; cat. no. 11587-1-AP; Proteintech Group, Inc.), PERK (1:1,000; cat. no. 24390-1-AP; Proteintech Group, Inc.), phosphorylated PERK (p-PERK) (1:1,000; cat. no. AP1501; ABclonal Biotech Co., Ltd.), ATF4 (1:10,000; cat. no. 81798-1-RR; Proteintech Group, Inc.), p-EIF2α (1:1,000; cat. no. AP0692; ABclonal Biotech Co., Ltd.), EIF2α (1:10,000; cat. no. 82936-1-PBS; Proteintech Group, Inc.), CHOP (1:1,000; cat. no. 15204-1-AP; Proteintech Group, Inc.), Caspase 3 (1:1,0000; cat. no. 82202-1-RR; Proteintech Group, Inc.), active-caspase-3 (1:1,000; cat. no. A11021; ABclonal Biotech Co., Ltd.), Bax (1:1,000; cat. no. A19684; ABclonal Biotech Co., Ltd.), Bcl-2 (1:1,000; cat. no. A19693; ABclonal Biotech Co., Ltd.).
Techniques: Western Blot, Negative Control
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