Journal: Acta neuropathologica

Article Title: Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

doi: 10.1007/s00401-012-1020-6

Figure Lengend Snippet: Fig. 2 Co-localization of Trn1 and FUS in all FTLD-FUS subtypes. Double-label immunofluorescence for FUS (red) and Trn1 (green), with DAPI staining of nuclei in the merged images. FUS-positive inclusions in all FTLD-FUS subtypes consistently showed co-localization of FUS and Trn1, as shown for neuronal cytoplasmic inclusions (NCI) and neuronal intranuclear inclusions (NII, arrow in a) in the dentate granule cells in aFTLD-U (a), NCI in the temporal cortex of NIFID (b) and NCI and glial cytoplasmic inclusions in the spinal cord of BIBD (c). Scale bar 10 lm

Article Snippet: Table 3 Trn1 cargo proteins with PY-NLS investigated in FTLD-FUS Protein name Antibody Physiological staining pattern Inclusions in FTLD-FUS Company Dilution Type FUS ProteinTech Group (60160-1-Ig) 1:1,000 MM nucl pos Sigma (HPA008784) 1:2,000 RP nucl pos TAF15 Bethyl Laboratories (IHC-00094-1) 1:200 RP nucl pos EWS Santa Cruz (clone G5) 1:200 MM nucl [ cyto pos Bethyl Laboratories (IHC-00086) 1:200 RP nucl [ cyto pos hnRNP A1 Santa Cruz (clone 4B10) 1:500 MM nucl neg hnRNP A0 Abcam (ab66661) 1:100 RP nucl neg hnRNP A2/B1 Sigma-Aldrich (clone DP3B3) 1:500 MM nucl neg hnRNP M3/M4 Santa Cruz (clone 2A6) 1:250 MM nucl neg hnRNP D ProteinTech Group (12770-1-AP) 1:500 RP nucl neg hnRNP H1 ProteinTech Group (14774-1-AP) 1:50 RP nucl [ cyto neg PQBP-1 ProteinTech Group (16264-1-AP) 1:250 RP nucl neg SAM68 ProteinTech Group (10222-1-AP) 1:250 RP nucl neg SLM-2 ProteinTech Group (13563-1-AP) 1:50 RP nucl neg HEXIM1 ProteinTech Group (15676-1-AP) 1:50 RP nucl neg RBM39 ProteinTech Group (21339-1-AP) 1:50 RP nucl [ cyto neg HuR Santa Cruz (clone 3A2) 1:250 MM nucl neg PABPN1 Abcam (EP3000Y) 1:1,000 RM nucl neg Cyto cytoplasmatic, MM mouse monoclonal, nucl nuclear, pos positive, RP rabbit polyclonal, RM rabbit monoclonal, neg negative Trn1 staining in neurological controls The normal and neurological control cases did not reveal Trn1 immunoreactive pathology with one exception (Table 2).

Techniques: Staining

Journal: Acta neuropathologica

Article Title: Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

doi: 10.1007/s00401-012-1020-6

Figure Lengend Snippet: Fig. 3 Absence of Trn1 pathology in ALS-FUS. Double-label immuno- fluorescence for FUS (red) and Trn1 (green), with DAPI staining of nuclei in the merged images. FUS-positive inclusions in ALS-FUS were not labeled for Trn1 as shown for neuronal cytoplasmic inclusions in the spinal cord for three different FUS mutations (a–c). Note the physiological nuclear staining for Trn1 in inclusion bearing cells. FUS-positive glial cytoplasmic inclusions present in a subset of ALS-FUS cases also showed no co-labeling for Trn1 (arrow in a). Scale bar 10 lm

Article Snippet: Table 3 Trn1 cargo proteins with PY-NLS investigated in FTLD-FUS Protein name Antibody Physiological staining pattern Inclusions in FTLD-FUS Company Dilution Type FUS ProteinTech Group (60160-1-Ig) 1:1,000 MM nucl pos Sigma (HPA008784) 1:2,000 RP nucl pos TAF15 Bethyl Laboratories (IHC-00094-1) 1:200 RP nucl pos EWS Santa Cruz (clone G5) 1:200 MM nucl [ cyto pos Bethyl Laboratories (IHC-00086) 1:200 RP nucl [ cyto pos hnRNP A1 Santa Cruz (clone 4B10) 1:500 MM nucl neg hnRNP A0 Abcam (ab66661) 1:100 RP nucl neg hnRNP A2/B1 Sigma-Aldrich (clone DP3B3) 1:500 MM nucl neg hnRNP M3/M4 Santa Cruz (clone 2A6) 1:250 MM nucl neg hnRNP D ProteinTech Group (12770-1-AP) 1:500 RP nucl neg hnRNP H1 ProteinTech Group (14774-1-AP) 1:50 RP nucl [ cyto neg PQBP-1 ProteinTech Group (16264-1-AP) 1:250 RP nucl neg SAM68 ProteinTech Group (10222-1-AP) 1:250 RP nucl neg SLM-2 ProteinTech Group (13563-1-AP) 1:50 RP nucl neg HEXIM1 ProteinTech Group (15676-1-AP) 1:50 RP nucl neg RBM39 ProteinTech Group (21339-1-AP) 1:50 RP nucl [ cyto neg HuR Santa Cruz (clone 3A2) 1:250 MM nucl neg PABPN1 Abcam (EP3000Y) 1:1,000 RM nucl neg Cyto cytoplasmatic, MM mouse monoclonal, nucl nuclear, pos positive, RP rabbit polyclonal, RM rabbit monoclonal, neg negative Trn1 staining in neurological controls The normal and neurological control cases did not reveal Trn1 immunoreactive pathology with one exception (Table 2).

Techniques: Staining, Labeling

Journal: Acta neuropathologica

Article Title: Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

doi: 10.1007/s00401-012-1020-6

Figure Lengend Snippet: Fig. 5 Absence of selected other Trn1 cargos (hnRNP A1, SAM68 and PABPN1) in FTLD-FUS. Double-label immunofluorescence for FUS (red) and other Trn1 cargos with PY-NLS (hnRNP A1, SAM68 and PABPN1, respectively, green) with DAPI staining of nuclei in the merged images in FTLD-FUS. FUS-positive inclusions in FTLD-FUS as shown here in the dentate gyrus of aFTLD-U were not labeled for hnRNP A1 (a), SAM68 (b) and PABPN1 (c). Scale bar 10 lm

Article Snippet: Table 3 Trn1 cargo proteins with PY-NLS investigated in FTLD-FUS Protein name Antibody Physiological staining pattern Inclusions in FTLD-FUS Company Dilution Type FUS ProteinTech Group (60160-1-Ig) 1:1,000 MM nucl pos Sigma (HPA008784) 1:2,000 RP nucl pos TAF15 Bethyl Laboratories (IHC-00094-1) 1:200 RP nucl pos EWS Santa Cruz (clone G5) 1:200 MM nucl [ cyto pos Bethyl Laboratories (IHC-00086) 1:200 RP nucl [ cyto pos hnRNP A1 Santa Cruz (clone 4B10) 1:500 MM nucl neg hnRNP A0 Abcam (ab66661) 1:100 RP nucl neg hnRNP A2/B1 Sigma-Aldrich (clone DP3B3) 1:500 MM nucl neg hnRNP M3/M4 Santa Cruz (clone 2A6) 1:250 MM nucl neg hnRNP D ProteinTech Group (12770-1-AP) 1:500 RP nucl neg hnRNP H1 ProteinTech Group (14774-1-AP) 1:50 RP nucl [ cyto neg PQBP-1 ProteinTech Group (16264-1-AP) 1:250 RP nucl neg SAM68 ProteinTech Group (10222-1-AP) 1:250 RP nucl neg SLM-2 ProteinTech Group (13563-1-AP) 1:50 RP nucl neg HEXIM1 ProteinTech Group (15676-1-AP) 1:50 RP nucl neg RBM39 ProteinTech Group (21339-1-AP) 1:50 RP nucl [ cyto neg HuR Santa Cruz (clone 3A2) 1:250 MM nucl neg PABPN1 Abcam (EP3000Y) 1:1,000 RM nucl neg Cyto cytoplasmatic, MM mouse monoclonal, nucl nuclear, pos positive, RP rabbit polyclonal, RM rabbit monoclonal, neg negative Trn1 staining in neurological controls The normal and neurological control cases did not reveal Trn1 immunoreactive pathology with one exception (Table 2).

Techniques: Staining, Labeling

Journal: Biomedicines

Article Title: Protective Effects of Trimetazidine and Dexmedetomidine on Liver Injury in a Mesenteric Artery Ischemia–Reperfusion Rat Model via Endoplasmic Reticulum Stress

doi: 10.3390/biomedicines12102299

Figure Lengend Snippet: Representative light microscopic screen images of liver tissue sections incubated with CHOP primary antibody. ( A ) (×20). Control group: In the liver tissue sections from the control group, hepatocytes with a normal structure (arrow) were observed to be CHOP-negative (CHOP positivity score: 0(0-0)). ( B ) (×20). I/R group: In the intralobular areas, primarily in Zone 1, hepatocytes showing intense CHOP positivity (spiral arrow) are observed (CHOP positivity score: 2(1-2)). ( C ) (×20). I/R+TMZ group: A decrease in hepatocytes showing CHOP positivity was observed in the perinobular areas, particularly in the intralobular regions (CHOP positivity score: 1(0-1)). ( D ) (×20). I/R+DEX group: A decrease in hepatocytes showing intense immunopositivity in the intralobular and perilobular areas was observed, with a widespread presence of CHOP-negative hepatocytes (arrow, CHOP positivity score: 0.5 (0-1)).

Article Snippet: The liver tissue slices were analyzed via immunohistochemistry labeling with primary antibodies against CHOP (E-AB-70087, 1/300, Elabscience, Houston, TX, USA), GPR78 (SC-13539, 1/350, Santa Cruz Biotechnology Inc. Dallas, TX, USA), 8-hydroxy-2′-deoxyguanosine (8-OHdG) (Santa Cruz, SC-66036, 1/200, Santa Cruz Biotechnology Inc., Dallas, TX, USA), cleaved caspase-3 (Abcam, ab4051, 1/100), anti-Bax ab32503, 1/200, (Abcam, Cambridge, UK), and anti-Bcl-2 (Abcam, ab32124, 1/200).

Techniques: Incubation, Control

Journal: eLife

Article Title: CB 1 R regulates soluble leptin receptor levels via CHOP, contributing to hepatic leptin resistance

doi: 10.7554/eLife.60771

Figure Lengend Snippet:

Article Snippet: Transfected construct ( Mus musculus ) , mCHOP-9E10 , Addgene , CHOP6: mCHOP-WT-9E10-pCDNA, #21913 RRID: Addgene_21913 , .

Techniques: Transfection, Construct, Plasmid Preparation, Luciferase, Enzyme-linked Immunosorbent Assay, Software

Journal: bioRxiv

Article Title: The tyrosine kinase inhibitor GNF-7 targets senescent cells through allosteric activation of GCN2

doi: 10.1101/2025.08.29.673062

Figure Lengend Snippet: ( A ) Dot plot representing significantly upregulated biological processes in senescent HT-1080 T cells upon treatment with GNF-7 (200nM, 6 h). The X-axis indicates the enrichment score (-Log10pvalue). For the analysis the top 100 hits sorted by False Discovery Rate (FDR) and with a positive log2FoldChange were selected. Senescence was induced by exposure to doxycycline (1μg/ml) for 6 days. ( B ) WB analysis of phosphorylated GCN2 (pGCN2) and CHOP levels in proliferating and senescent HT’1080 cells. GNF-7 was used at the indicated concentrations for 3 h. Senescence was induced by palbociclib (5 μM) for 7 days. ( C ) Heatmap representing the EGFP median fluorescence intensity in DLD-1-CHOP EGFP cells after treatment with the indicated drugs for 24 h. Cells were infected with mCherry-expressing lentiviral vectors carrying sgRNAs to target the four ISR kinases. Control cells were infected with the empty vector. EGFP MFI ratios between mCherry-positive (KO) and negative (WT) cells were calculated and normalized to the control levels for each treatment: untreated, Tigecycline 10 μM, Neratinib 500 nM and GNF-7 200 nM. ( D ) Competition assay in wild type (red, WT) and GCN2-deficient (green, GCN2 KO ) HT-1080 cells cultured for 12 days in the presence or absence of 50 nM GNF-7. (E) Percentage of apoptotic cells (measured by FACS as Annexin V positive and PI negative) upon GNF-7 treatment in proliferating cells, as well as senescent WT and GCN2 KO HT-1080 cells. Senescence was induced by palbociclib (5 μM) for 7 days. GNF-7 was used at the indicated doses, and navitoclax at 5 μM.

Article Snippet: To monitor CHOP levels, we used the lentiviral plasmid pCLX-CHOP-dGFP (Addgene, 71299).

Techniques: Fluorescence, Infection, Expressing, Control, Plasmid Preparation, Competitive Binding Assay, Cell Culture

Journal: Biomedicines

Article Title: Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response

doi: 10.3390/biomedicines8100384

Figure Lengend Snippet: Effect of idebenone on mRNA levels of endoplasmic reticulum stress (ER stress) markers in the Winnie model of spontaneous chronic colitis. Gene expression of ( A ) GRP78 , ( B ) CHOP , ( C ) ATF6 , ( D ) XBP-1 and ( E ) PERK were determined for the proximal (PC) and distal colon (DC) for all the three groups. mRNA levels were normalized to GAPDH gene and presented as fold change. Data represents mean ± SEM ( n = 4/group) using One-way ANOVA followed by Tukey’s post-test, where * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: The membranes were then blocked in 5% skim milk prepared in TBST and incubated overnight with primary antibody against NQO-1 (1:1000, ab34173, Abcam), GRP78 (1:1000, NBP1-06274, Novus biologicals) CHOP (1:1000, NBP2-13172, Novus Biologicals) and β-actin (1:8000, NB600-503, Novus Biologicals) at 4 °C.

Techniques: Gene Expression

Journal: Biomedicines

Article Title: Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response

doi: 10.3390/biomedicines8100384

Figure Lengend Snippet: Effect of idebenone on endoplasmic reticulum stress (ER stress) and oxidative stress in spontaneous chronic colitis in Winnie mice. ( A ) protein levels of GRP78, CHOP and NQO-1 were analyzed using western blotting of tissue samples from the distal colon (DC), ( B – D ) densitometry of GRP78, CHOP and NQO-1 expression. Band densities were normalized to β-actin. ( E ) malondialdehyde (MDA) levels in distal colon. Data represents mean ± SEM ( n = 3/group). Statistical significance was determined using One-way ANOVA followed by Tukey’s post-test, where * p < 0.05, ** p < 0.01 and *** p < 0.001 and ns: nonsignificant.

Article Snippet: The membranes were then blocked in 5% skim milk prepared in TBST and incubated overnight with primary antibody against NQO-1 (1:1000, ab34173, Abcam), GRP78 (1:1000, NBP1-06274, Novus biologicals) CHOP (1:1000, NBP2-13172, Novus Biologicals) and β-actin (1:8000, NB600-503, Novus Biologicals) at 4 °C.

Techniques: Western Blot, Expressing