chi3l1  (Proteintech)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Hepatocyte Growth Factor Confers Trabecular Meshwork Stem Cell Resilience and Paracrine Protection of Trabecular Meshwork Cells in Glaucoma

doi: 10.1167/iovs.67.4.1

Figure Lengend Snippet: Characterization of TM and TMSC markers under TGF-β2 treatment. ( A–D ) CHI3L1 and MYOC expression in TM cells treated with dexamethasone (Dex, 100 nM, 5 days). Immunofluorescent staining is shown in ( A ), quantitative analyses in ( B ), Western blotting results in ( C ), and densitometric analyses in ( D ). ( E–H ) CHI3L1 expression in TM cells and TMSCs and OCT4 expression in TMSCs under control and TGF-β2 (3 ng/mL, 5 days) conditions. Immunofluorescent staining is shown in ( E , F ), and corresponding quantitative analyses are shown in ( G , H ). ( I–L ) Western blotting analysis of CHI3L1 and OCT4 expression in TMSCs ( I ) and CHI3L1 expression in TM cells ( K ) under control and TGF-β2 treatment, with densitometric analyses shown in ( J–L ). Data are presented as mean ± SD ( n ≥ 3). Statistical analyses were performed using two-tailed unpaired t -tests. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Membranes were blocked with EveryBlot Blocking Buffer (Bio-Rad, Hercules, CA, USA) for 5 minutes at room temperature and incubated overnight at 4°C with primary antibodies against CHI3L1 (R&D Systems, 1:500), MYOC (a kind gift from Dr. W. Daniel Stamer, Duke University, 1:3000), OCT4 (Cell Signaling Technology, Danvers, MA, USA; 1:1000), CTGF (Santa Cruz Biotechnology, 1:1000), α-SMA (R&D Systems, 1:1000), fibronectin (FN, Abcam, 1:1000), and GAPDH (Cell Signaling Technology, 1:2000).

Techniques: Expressing, Staining, Western Blot, Control, Two Tailed Test

Journal: Kidney International Reports

Article Title: Kidney Injury Urine Biomarker Normal Ranges in Children

doi: 10.1016/j.ekir.2026.106348

Figure Lengend Snippet: LMS percentiles for urinary (a) KIM-1, (b, boys; c, girls) NGAL, (d, boys; e, girls) DKK3, and (f) CHI3L1 to creatinine ratios in the HARP cohort. The 5th, 10th, 25th (dashed line), 50th (bold line), 75th (dashed line), 90th, and 95th percentiles are given. Data on boys and girls is given as blue triangles and orange dots, respectively. CHI3L1, chitinase 3-like protein-1; crea, creatinine; DKK3, dickkopf-3; KIM-1, kidney-injury molecule-1; LMS, lambda-mu-sigma; NGAL, neutrophil gelatinase-associated lipocalin.

Article Snippet: Urinary concentrations of KIM-1 (Quantikine ELISA, R&D system; MN Catalog No. DKM100; sensitivity 0.046 ng/ml), NGAL (Quantikine ELISA, R&D system; MN, Catalog No. DLCN20; sensitivity 0.04 ng/ml), DKK3 (Duo Set ELISA, R&D system; MN, Catalog No. DY1118, sensitivity 31.2 pg/ml), CHI3L1 (Quantikine ELISA, R&D system; MN, Catalog No. DC3L10; sensitivity 8.15 pg/ml), MCP-1 (Quantikine ELISA, R&D system;MN, Catalog No. DCP00; sensitivity 10 pg/ml), PIIINP (Novus Biologicals, Wiesbaden Nordenstadt, Germany, Catalog No. NBP2-76434; sensitivity 14.06 pg/ml), and EGF (Quantikine ELISA, R&D system; MN, Catalog No. DEG00; sensitivity 0.7 pg/ml) were measured using ELISA, according to the manufacturer’s protocols.

Techniques: