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ccrf-cem  (ATCC)


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    ATCC ccrf-cem
    Ccrf Cem, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 878 article reviews
    ccrf-cem - by Bioz Stars, 2026-06
    96/100 stars

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    Gene expression analysis induced by P3C.1 and P3C.2 in three different cell lines. ( A ) Heatmap of differentially expressed genes of MDA-MB-231, ( B ) Jurkat and ( C ) CEM cells treated with P3C.1 and P3C.2, or with solvent control (DMSO). Each column represents the expression values of the different treatments performed for 6 h for each corresponding cell line, and each row denotes data from one transcript. The color legend of each expression values (log2 fold change) is displayed on the right. ( D ) Differentially expressed genes; up-regulated (green bars) and down-regulated (red bars) obtained for each cell line and treatments, considering only transcripts with a log2 fold change of >1.0, and a p -adjusted value of <0.05.

    Journal: Oncology Research

    Article Title: Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling Pathways

    doi: 10.32604/or.2026.074945

    Figure Lengend Snippet: Gene expression analysis induced by P3C.1 and P3C.2 in three different cell lines. ( A ) Heatmap of differentially expressed genes of MDA-MB-231, ( B ) Jurkat and ( C ) CEM cells treated with P3C.1 and P3C.2, or with solvent control (DMSO). Each column represents the expression values of the different treatments performed for 6 h for each corresponding cell line, and each row denotes data from one transcript. The color legend of each expression values (log2 fold change) is displayed on the right. ( D ) Differentially expressed genes; up-regulated (green bars) and down-regulated (red bars) obtained for each cell line and treatments, considering only transcripts with a log2 fold change of >1.0, and a p -adjusted value of <0.05.

    Article Snippet: In addition, the RAMOS (ATCC, CRL-1596), CEM (ATCC, CCL-119), JURKAT (ATCC, TIB-152), NALM-6 (ATCC, CRL-3273), RPMI-8226, MM.1S, MM.1R, U266, Rec-1, JVM-13, A549 (ATCC, CRM-CCL-185), HCC70 (ATCC, CRL-2315), HCC1419 (ATCC, CRL-2326), T47D (ATCC, CRL-2865), KMS-11, and OVCAR-5 cell lines were grown in Roswell Park Memorial Institute-1640 (RPMI-1640) culture media (Cytiva-Hyclone, SH30027FS, UT, USA,) supplemented with 10% FBS, 100 U/mL of penicillin, and 100 μg/mL of streptomycin.

    Techniques: Gene Expression, Solvent, Control, Expressing

    P3C.1 and P3C.2 differentially expressed genes induced in common. ( A ) MDA-MB-231, ( B ) Jurkat, and CEM common DEGs identified for both compounds. ( C ) P3C.1 and P3C.2 induced 4 up-regulated genes in common (DUSP8, KLF6, KLF7 and BACH2) in MDA-MB-231, Jurkat and CEM cell lines.

    Journal: Oncology Research

    Article Title: Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling Pathways

    doi: 10.32604/or.2026.074945

    Figure Lengend Snippet: P3C.1 and P3C.2 differentially expressed genes induced in common. ( A ) MDA-MB-231, ( B ) Jurkat, and CEM common DEGs identified for both compounds. ( C ) P3C.1 and P3C.2 induced 4 up-regulated genes in common (DUSP8, KLF6, KLF7 and BACH2) in MDA-MB-231, Jurkat and CEM cell lines.

    Article Snippet: In addition, the RAMOS (ATCC, CRL-1596), CEM (ATCC, CCL-119), JURKAT (ATCC, TIB-152), NALM-6 (ATCC, CRL-3273), RPMI-8226, MM.1S, MM.1R, U266, Rec-1, JVM-13, A549 (ATCC, CRM-CCL-185), HCC70 (ATCC, CRL-2315), HCC1419 (ATCC, CRL-2326), T47D (ATCC, CRL-2865), KMS-11, and OVCAR-5 cell lines were grown in Roswell Park Memorial Institute-1640 (RPMI-1640) culture media (Cytiva-Hyclone, SH30027FS, UT, USA,) supplemented with 10% FBS, 100 U/mL of penicillin, and 100 μg/mL of streptomycin.

    Techniques:

    Ingenuity pathways analysis of genes commonly found in P3C.1 and P3C.2-treated MDA-MB231, JURKAT, and CEM cells, identified canonical pathways implicating these genes in kinase-mediated signal transduction (see green asterisks * ), including RAF-independent MAPK1/3 activation, RAF/MAP kinase cascade, Protein Kinase A Signaling, and the SAPK/JNK signaling pathway, as well as cell signaling processes (see blue asterisks * ), and membrane dynamics (see blue asterisks * ). The figure shown was recreated from the original IPA histogram to enlarge features and improve legibility.

    Journal: Oncology Research

    Article Title: Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling Pathways

    doi: 10.32604/or.2026.074945

    Figure Lengend Snippet: Ingenuity pathways analysis of genes commonly found in P3C.1 and P3C.2-treated MDA-MB231, JURKAT, and CEM cells, identified canonical pathways implicating these genes in kinase-mediated signal transduction (see green asterisks * ), including RAF-independent MAPK1/3 activation, RAF/MAP kinase cascade, Protein Kinase A Signaling, and the SAPK/JNK signaling pathway, as well as cell signaling processes (see blue asterisks * ), and membrane dynamics (see blue asterisks * ). The figure shown was recreated from the original IPA histogram to enlarge features and improve legibility.

    Article Snippet: In addition, the RAMOS (ATCC, CRL-1596), CEM (ATCC, CCL-119), JURKAT (ATCC, TIB-152), NALM-6 (ATCC, CRL-3273), RPMI-8226, MM.1S, MM.1R, U266, Rec-1, JVM-13, A549 (ATCC, CRM-CCL-185), HCC70 (ATCC, CRL-2315), HCC1419 (ATCC, CRL-2326), T47D (ATCC, CRL-2865), KMS-11, and OVCAR-5 cell lines were grown in Roswell Park Memorial Institute-1640 (RPMI-1640) culture media (Cytiva-Hyclone, SH30027FS, UT, USA,) supplemented with 10% FBS, 100 U/mL of penicillin, and 100 μg/mL of streptomycin.

    Techniques: Transduction, Activation Assay, Membrane

    Connectivity map analyses revealed strong connectivity of P3C compounds with tubulin inhibitors. The top 20 perturbagens identified using P3C.1 and P3C.2 genes in common for ( A ) MDA-MB-231, and ( B ) Jurkat, and CEM cell lines. Tau scores of 90 or above have been previously recognized as strong and suitable for further investigation ( https://clue.io ).

    Journal: Oncology Research

    Article Title: Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling Pathways

    doi: 10.32604/or.2026.074945

    Figure Lengend Snippet: Connectivity map analyses revealed strong connectivity of P3C compounds with tubulin inhibitors. The top 20 perturbagens identified using P3C.1 and P3C.2 genes in common for ( A ) MDA-MB-231, and ( B ) Jurkat, and CEM cell lines. Tau scores of 90 or above have been previously recognized as strong and suitable for further investigation ( https://clue.io ).

    Article Snippet: In addition, the RAMOS (ATCC, CRL-1596), CEM (ATCC, CCL-119), JURKAT (ATCC, TIB-152), NALM-6 (ATCC, CRL-3273), RPMI-8226, MM.1S, MM.1R, U266, Rec-1, JVM-13, A549 (ATCC, CRM-CCL-185), HCC70 (ATCC, CRL-2315), HCC1419 (ATCC, CRL-2326), T47D (ATCC, CRL-2865), KMS-11, and OVCAR-5 cell lines were grown in Roswell Park Memorial Institute-1640 (RPMI-1640) culture media (Cytiva-Hyclone, SH30027FS, UT, USA,) supplemented with 10% FBS, 100 U/mL of penicillin, and 100 μg/mL of streptomycin.

    Techniques: