jurkat t cells  (ATCC)

Journal: Oncology Letters

Article Title: BUD23 is associated with malignancy and correlates with immune infiltration in NSCLC

doi: 10.3892/ol.2026.15608

Figure Lengend Snippet: BUD23 knockdown suppresses the proliferative and migration of NSCLC cells. (A) RT-qPCR was used to quantify BUD23 mRNA levels in HBE cells and a panel of NSCLC cell lines. Validation of BUD23 knockdown efficiency in (B) A549 and (C) H1299 cells by RT-qPCR. Assessment of cell viability in (D) A549 and (E) H1299 cells via CCK-8 assay and cell migration in (F) A549 and (G) H1299 cells by wound healing assay (magnification, ×10). CCK-8 assays of (H) A549 and (I) H1299 cells 24 h after BUD23 knockdown with or without subsequent co-culture with Jurkat T cells for 48 h in Transwell chambers. (J) Quantification of apoptosis in A549 and H1299 cells via Annexin V/PI flow cytometry. **P<0.01 and ***P<0.001 vs. HBE, NC, Con or as indicated. NSCLC, non-small cell lung cancer; RT-qPCR, reverse transcription-quantitative PCR; HBE, human bronchial epithelial; CCK-8, Cell Counting Kit-8; NC, negative control; Con, control; Si1/2, small interfering RNA targeting BUD23.

Article Snippet: The HBE [full name: HBE4-E6/E7 (Human Bronchial Epithelial Cells; cat. no. CRL-2078)] cell line, A549 lung adenocarcinoma cell line (cat. no. CCL-185), H1299 lung large cell carcinoma cell line (cat. no. CRL-5803), H460 lung large cell carcinoma cell line (cat. no. HTB-177) and Jurkat T cells (cat. no. TIB-152; a childhood T acute lymphoblastic leukemia T-cell line), were purchased from the American Type Culture Collection.

Techniques: Knockdown, Migration, Quantitative RT-PCR, Biomarker Discovery, CCK-8 Assay, Wound Healing Assay, Co-Culture Assay, Flow Cytometry, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Counting, Negative Control, Control, Small Interfering RNA

Journal: Current Protocols

Article Title: Engineering, Expression, Purification, and Application of Glycosaminoglycan‐Specific Antibodies

doi: 10.1002/cpz1.70358

Figure Lengend Snippet: Use of anti‐HS scFvs as staining reagents for flow cytometry. ( A‐C ) Workflow, gating strategy, and representative histograms for FACS analyses of ( A ) live Jurkat E6‐1 cells stained with anti‐HS scFv‐H HS001 and PE‐conjugated anti‐His antibody (data collected on Sony SA3800 spectral analyzer); ( B ) Vero cells stained with anti‐HS scFv‐H HS001, then fixed and detected using AlexaFluor647‐conjugated anti‐human IgG antibody (data collected on Novocyte Quanteon in AlexaFluor647 channel); and ( C ) live Vero cells stained with TAMRA‐conjugated anti‐HS scFvs (data collected on Novocyte Quanteon in the PE/TAMRA channel). HS034 is a negative control scFv and has no known epitope. scFv staining observed in live, single‐cell populations.

Article Snippet: Figure shows representative data as heatmaps for % live cells showing scFv staining obtained using the FACS staining protocol detailed in Basic Protocol for two cell lines: HEK293 cells (ATCC, CRL‐1573, maintained in DMEM supplemented with l ‐glutamine, glucose, sodium pyruvate, and 10% FBS) and Jurkat E6‐1 cells (ATCC, TIB‐152, maintained in IMDM with GlutaMAX and 10% FBS).

Techniques: Staining, Flow Cytometry, Negative Control, Single Cell

Journal: Current Protocols

Article Title: Engineering, Expression, Purification, and Application of Glycosaminoglycan‐Specific Antibodies

doi: 10.1002/cpz1.70358

Figure Lengend Snippet: Data for qualitative validation of the anti‐HS scFv‐H panel. Representative data for % live cells showing scFv staining observed for HEK293 and Jurkat E6‐1 cells, obtained following indirect FACS staining as in Basic Protocol ).

Article Snippet: Figure shows representative data as heatmaps for % live cells showing scFv staining obtained using the FACS staining protocol detailed in Basic Protocol for two cell lines: HEK293 cells (ATCC, CRL‐1573, maintained in DMEM supplemented with l ‐glutamine, glucose, sodium pyruvate, and 10% FBS) and Jurkat E6‐1 cells (ATCC, TIB‐152, maintained in IMDM with GlutaMAX and 10% FBS).

Techniques: Biomarker Discovery, Staining