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caspase1 antibody  (Proteintech)


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    Structured Review

    Proteintech caspase1 antibody
    Caspase1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase1/Caspase+1%2Fp20%2Fp10+Antibody/pmc12995703-8-0-3
    Average 96 stars, based on 1683 article reviews
    caspase1 antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Exploring the mechanism of Cucurbitacin B against myocardial ischemia reperfusion injury based on network pharmacology and experimental validation.
    Article Snippet: Myocardial ischemia/reperfusion injury (MI/RI) is a complex state involving numerous inflammatory mediators, which significantly worsens the outcomes of patients with cardiovascular diseases.. However, no standardized treatment plan for MI/RI prevails at present.. Cucurbitacin B (Cb-B) is a natural bioactive substance extracted from the stem of melon flowers, and is associated with Wnt-β-Catenin, NF-κB, and PI3K/AKT signaling pathways to initiate apoptosis and inflammatory responses in various cancers.

    Membrane:

    Article Title: Exploring the mechanism of Cucurbitacin B against myocardial ischemia reperfusion injury based on network pharmacology and experimental validation.
    Article Snippet: Myocardial ischemia/reperfusion injury (MI/RI) is a complex state involving numerous inflammatory mediators, which significantly worsens the outcomes of patients with cardiovascular diseases.. However, no standardized treatment plan for MI/RI prevails at present.. Cucurbitacin B (Cb-B) is a natural bioactive substance extracted from the stem of melon flowers, and is associated with Wnt-β-Catenin, NF-κB, and PI3K/AKT signaling pathways to initiate apoptosis and inflammatory responses in various cancers.

    Incubation:

    Article Title: Exploring the mechanism of Cucurbitacin B against myocardial ischemia reperfusion injury based on network pharmacology and experimental validation.
    Article Snippet: Myocardial ischemia/reperfusion injury (MI/RI) is a complex state involving numerous inflammatory mediators, which significantly worsens the outcomes of patients with cardiovascular diseases.. However, no standardized treatment plan for MI/RI prevails at present.. Cucurbitacin B (Cb-B) is a natural bioactive substance extracted from the stem of melon flowers, and is associated with Wnt-β-Catenin, NF-κB, and PI3K/AKT signaling pathways to initiate apoptosis and inflammatory responses in various cancers.

    Article Title: Carbon-based nanomaterials induce ovarian dysfunction via NLRP3 inflammasome-mediated pyroptosis in granulosa cells.
    Article Snippet: .. After blocking with 5% BSA for 1 h, the membranes were incubated with different primary antibody overnight at 4°C: Bax (A0207, ABclonal), Bcl2 (A0208, ABclonal), Caspase3 (A11319, ABclonal), NLPR3 (19771-1-AP, Proteintech), ASC (A22046, ABclonal), Caspase1 (22915-1-AP, Proteintech), IL-1b (26048-1-AP, Proteintech), HO-1 (10701-1-AP, Proteintech), NRF2 (A21176, ABclonal), CD206 (18704-1-AP, Proteintech), iNOS (A3774, ABclonal), bactin (AC026, ABclonal). .. Next, they were incubated with secondary antibodies (dilution 1:5000, ABclonal), and the signals were detected by the enhanced ECL system (Bio-Rad, USA).

    Article Title: Irisolidone Ameliorates Cyclophosphamide-Induced POI via Inhibiting Inflammatory Response.
    Article Snippet: .. After cooling, the sections were incubated with 3% hydrogen peroxide for 10– 15 minutes to block endogenous peroxidase activity, followed by blocking with 5% BSA for 30 minutes. phosphorylated nuclear factor kappa B (p-NFκB; 1:200, Proteintech, Cat. No. 10745-1-AP, Wuhan, Hubei, China), NOD-like receptor pyrin domain-containing 3 (NLRP3; 1:200, ABclonal, Cat. No. A5652, Wuhan, Hubei, China), and Caspase1 (1:200, Proteintech, Cat No. 22915-1-AP, Wuhan, China) were added, and the sectionswere incubated overnight at 4 °C. .. Subsequent to this, the sections were incubated with the polymer HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Cat: RGAR011, Proteintech, Wuhan, China) at room temperature for 60 minutes.

    Article Title: Irisolidone Ameliorates Cyclophosphamide-Induced POI via Inhibiting Inflammatory Response.
    Article Snippet: .. This was followed by overnight incubation at 4 °C with primary antibodies against the following targets: glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, ABclonal, Cat. No. AC002, Wuhan, China), phosphorylated nuclear factor kappa B (p-NFκB; 1:1000, Proteintech, Cat. No. 10745-1-AP,Wuhan, China), NOD-like receptor pyrin domain-containing 3 (NLRP3; 1:1000, ABclonal, Cat. No. A5652, Wuhan, China), interleukin-1β (IL1β; 1:1000, ABclonal, Cat. No. A16288, Wuhan, China), and Caspase1 (1:1000, Proteintech, Cat No. 22915-1-AP, Wuhan, China). ..

    Article Title: Bionic Nanoparticle Hydrogel for Astrocyte-Targeted Estradiol Delivery Ameliorates Perimenopausal Depression via P2X7 receptor Inhibition
    Article Snippet: .. Specific primary antibodies against NLRP3 (ab263899, Abcam), GSDMD, Caspase1 (31020-1-AP, Proteintech), or GAPDH (60004-1-Ig, Proteintech) were incubated at 4°C. ..

    Blocking Assay:

    Article Title: Carbon-based nanomaterials induce ovarian dysfunction via NLRP3 inflammasome-mediated pyroptosis in granulosa cells.
    Article Snippet: .. After blocking with 5% BSA for 1 h, the membranes were incubated with different primary antibody overnight at 4°C: Bax (A0207, ABclonal), Bcl2 (A0208, ABclonal), Caspase3 (A11319, ABclonal), NLPR3 (19771-1-AP, Proteintech), ASC (A22046, ABclonal), Caspase1 (22915-1-AP, Proteintech), IL-1b (26048-1-AP, Proteintech), HO-1 (10701-1-AP, Proteintech), NRF2 (A21176, ABclonal), CD206 (18704-1-AP, Proteintech), iNOS (A3774, ABclonal), bactin (AC026, ABclonal). .. Next, they were incubated with secondary antibodies (dilution 1:5000, ABclonal), and the signals were detected by the enhanced ECL system (Bio-Rad, USA).

    Article Title: Irisolidone Ameliorates Cyclophosphamide-Induced POI via Inhibiting Inflammatory Response.
    Article Snippet: .. After cooling, the sections were incubated with 3% hydrogen peroxide for 10– 15 minutes to block endogenous peroxidase activity, followed by blocking with 5% BSA for 30 minutes. phosphorylated nuclear factor kappa B (p-NFκB; 1:200, Proteintech, Cat. No. 10745-1-AP, Wuhan, Hubei, China), NOD-like receptor pyrin domain-containing 3 (NLRP3; 1:200, ABclonal, Cat. No. A5652, Wuhan, Hubei, China), and Caspase1 (1:200, Proteintech, Cat No. 22915-1-AP, Wuhan, China) were added, and the sectionswere incubated overnight at 4 °C. .. Subsequent to this, the sections were incubated with the polymer HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Cat: RGAR011, Proteintech, Wuhan, China) at room temperature for 60 minutes.

    Western Blot:

    Article Title: Antihypertension and Improvement Effects of Hexapeptide APPLRP on Exosomes-Mediated Vascular Endothelial Inflammation in Spontaneously Hypertensive Rats.
    Article Snippet: The hexapeptide APPLRP from the edible Grifola frondosa mushroom has been verified to possess significant ACE inhibitory activity and ameliorates vascular remodeling.. However, in vivo effects have not been systematically investigated.. The present study aimed to explore the in vivo mechanisms of antihypertensive peptide APPLRP.

    Activity Assay:

    Article Title: Irisolidone Ameliorates Cyclophosphamide-Induced POI via Inhibiting Inflammatory Response.
    Article Snippet: .. After cooling, the sections were incubated with 3% hydrogen peroxide for 10– 15 minutes to block endogenous peroxidase activity, followed by blocking with 5% BSA for 30 minutes. phosphorylated nuclear factor kappa B (p-NFκB; 1:200, Proteintech, Cat. No. 10745-1-AP, Wuhan, Hubei, China), NOD-like receptor pyrin domain-containing 3 (NLRP3; 1:200, ABclonal, Cat. No. A5652, Wuhan, Hubei, China), and Caspase1 (1:200, Proteintech, Cat No. 22915-1-AP, Wuhan, China) were added, and the sectionswere incubated overnight at 4 °C. .. Subsequent to this, the sections were incubated with the polymer HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Cat: RGAR011, Proteintech, Wuhan, China) at room temperature for 60 minutes.



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    Image Search Results


    Cytosolic mtDNA levels and activation of the NLRP3 inflammasome and pyroptosis in the hearts of DCM mice. (A) mRNA levels of D-loop1, CytB, 16S, and ND4, determined by qRT-PCR. (B,C) Protein expression and quantitative analysis of NLRP3, ASC, caspase-1 (p20/pro-caspase-1), and GSDMD- N/GSDMD, as well as IL-1ẞ and IL-18 in mouse hearts, detected by Western blotting. Data are presented as the mean ± SE. # P<0.05 vs. db/m group.

    Journal: Frontiers in Pharmacology

    Article Title: Icariin attenuates diabetic cardiomyopathy by inhibiting NLRP3 inflammasome through SIRT3-mediated TFAM deacetylation

    doi: 10.3389/fphar.2026.1829772

    Figure Lengend Snippet: Cytosolic mtDNA levels and activation of the NLRP3 inflammasome and pyroptosis in the hearts of DCM mice. (A) mRNA levels of D-loop1, CytB, 16S, and ND4, determined by qRT-PCR. (B,C) Protein expression and quantitative analysis of NLRP3, ASC, caspase-1 (p20/pro-caspase-1), and GSDMD- N/GSDMD, as well as IL-1ẞ and IL-18 in mouse hearts, detected by Western blotting. Data are presented as the mean ± SE. # P<0.05 vs. db/m group.

    Article Snippet: The membranes were blocked with 5% skimmed milk at room temperature for 1 h, followed by an overnight incubation at 4 °C with the following primary antibodies: SIRT3 (1:1,000, Cell Signaling Technology); TFAM (1:800, Santa Cruz Biotechnology); NLRP3 (1:2000, Proteintech Group); ASC (1:1,000, ABclonal Technology); Caspase1 p20 (1:1,000, AdipoGen); pro-Caspase1 (1:1,000, MedChemExpress); GSDMD-N (1:2000, Immunoway); GSDMD (1:1,000, Affinity); IL-1β (1:2000, Proteintech); IL-18 (1:2000, Proteintech) and VDAC1 (1:500, Affinity) and β-Tubulin (1:50,000, Affinity).

    Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Western Blot

    ICA is associated with reduced cytosolic mtDNA release, NLRP3 inflammasome activation, and pyroptosis in DCM mice, which may occur in a SIRT3-dependent manner. (A–D) mRNA levels of D-loopl, CytB, 16S, and ND4 were detected by qRT-PCR. (E–K) Protein expression and quantitative analysis of NLRP3, ASC, caspase-1 p20, GSDMD-N, IL-1ẞ, and IL-18 in mouse heart tissue were detected by Western blotting. (L) Representative TEM images of pyroptosis in H9c2 cells (original magnification: x7,000). The red arrow indicates the mitochondria; the orange arrow indicates the rough endoplasmic reticulum; the green arrow indicates the secondary lysosome; the purple arrow indicates the pyroptosis body. Data are presented as the mean ± SE. # P<0.05 vs. db/m group; *P<0.05 vs. db/db group; **P<0.05 vs. ICA group.

    Journal: Frontiers in Pharmacology

    Article Title: Icariin attenuates diabetic cardiomyopathy by inhibiting NLRP3 inflammasome through SIRT3-mediated TFAM deacetylation

    doi: 10.3389/fphar.2026.1829772

    Figure Lengend Snippet: ICA is associated with reduced cytosolic mtDNA release, NLRP3 inflammasome activation, and pyroptosis in DCM mice, which may occur in a SIRT3-dependent manner. (A–D) mRNA levels of D-loopl, CytB, 16S, and ND4 were detected by qRT-PCR. (E–K) Protein expression and quantitative analysis of NLRP3, ASC, caspase-1 p20, GSDMD-N, IL-1ẞ, and IL-18 in mouse heart tissue were detected by Western blotting. (L) Representative TEM images of pyroptosis in H9c2 cells (original magnification: x7,000). The red arrow indicates the mitochondria; the orange arrow indicates the rough endoplasmic reticulum; the green arrow indicates the secondary lysosome; the purple arrow indicates the pyroptosis body. Data are presented as the mean ± SE. # P<0.05 vs. db/m group; *P<0.05 vs. db/db group; **P<0.05 vs. ICA group.

    Article Snippet: The membranes were blocked with 5% skimmed milk at room temperature for 1 h, followed by an overnight incubation at 4 °C with the following primary antibodies: SIRT3 (1:1,000, Cell Signaling Technology); TFAM (1:800, Santa Cruz Biotechnology); NLRP3 (1:2000, Proteintech Group); ASC (1:1,000, ABclonal Technology); Caspase1 p20 (1:1,000, AdipoGen); pro-Caspase1 (1:1,000, MedChemExpress); GSDMD-N (1:2000, Immunoway); GSDMD (1:1,000, Affinity); IL-1β (1:2000, Proteintech); IL-18 (1:2000, Proteintech) and VDAC1 (1:500, Affinity) and β-Tubulin (1:50,000, Affinity).

    Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Western Blot