Journal: Frontiers in Pharmacology

Article Title: Icariin attenuates diabetic cardiomyopathy by inhibiting NLRP3 inflammasome through SIRT3-mediated TFAM deacetylation

doi: 10.3389/fphar.2026.1829772

Figure Lengend Snippet: Cytosolic mtDNA levels and activation of the NLRP3 inflammasome and pyroptosis in the hearts of DCM mice. (A) mRNA levels of D-loop1, CytB, 16S, and ND4, determined by qRT-PCR. (B,C) Protein expression and quantitative analysis of NLRP3, ASC, caspase-1 (p20/pro-caspase-1), and GSDMD- N/GSDMD, as well as IL-1ẞ and IL-18 in mouse hearts, detected by Western blotting. Data are presented as the mean ± SE. # P<0.05 vs. db/m group.

Article Snippet: The membranes were blocked with 5% skimmed milk at room temperature for 1 h, followed by an overnight incubation at 4 °C with the following primary antibodies: SIRT3 (1:1,000, Cell Signaling Technology); TFAM (1:800, Santa Cruz Biotechnology); NLRP3 (1:2000, Proteintech Group); ASC (1:1,000, ABclonal Technology); Caspase1 p20 (1:1,000, AdipoGen); pro-Caspase1 (1:1,000, MedChemExpress); GSDMD-N (1:2000, Immunoway); GSDMD (1:1,000, Affinity); IL-1β (1:2000, Proteintech); IL-18 (1:2000, Proteintech) and VDAC1 (1:500, Affinity) and β-Tubulin (1:50,000, Affinity).

Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Icariin attenuates diabetic cardiomyopathy by inhibiting NLRP3 inflammasome through SIRT3-mediated TFAM deacetylation

doi: 10.3389/fphar.2026.1829772

Figure Lengend Snippet: ICA is associated with reduced cytosolic mtDNA release, NLRP3 inflammasome activation, and pyroptosis in DCM mice, which may occur in a SIRT3-dependent manner. (A–D) mRNA levels of D-loopl, CytB, 16S, and ND4 were detected by qRT-PCR. (E–K) Protein expression and quantitative analysis of NLRP3, ASC, caspase-1 p20, GSDMD-N, IL-1ẞ, and IL-18 in mouse heart tissue were detected by Western blotting. (L) Representative TEM images of pyroptosis in H9c2 cells (original magnification: x7,000). The red arrow indicates the mitochondria; the orange arrow indicates the rough endoplasmic reticulum; the green arrow indicates the secondary lysosome; the purple arrow indicates the pyroptosis body. Data are presented as the mean ± SE. # P<0.05 vs. db/m group; *P<0.05 vs. db/db group; **P<0.05 vs. ICA group.

Article Snippet: The membranes were blocked with 5% skimmed milk at room temperature for 1 h, followed by an overnight incubation at 4 °C with the following primary antibodies: SIRT3 (1:1,000, Cell Signaling Technology); TFAM (1:800, Santa Cruz Biotechnology); NLRP3 (1:2000, Proteintech Group); ASC (1:1,000, ABclonal Technology); Caspase1 p20 (1:1,000, AdipoGen); pro-Caspase1 (1:1,000, MedChemExpress); GSDMD-N (1:2000, Immunoway); GSDMD (1:1,000, Affinity); IL-1β (1:2000, Proteintech); IL-18 (1:2000, Proteintech) and VDAC1 (1:500, Affinity) and β-Tubulin (1:50,000, Affinity).

Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Western Blot