medium bactotm casitone  (Difco)

Journal: International Journal of Molecular Sciences

Article Title: Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production in Levilactobacillus brevis CRL 2013

doi: 10.3390/ijms27010082

Figure Lengend Snippet: Growth medium acidification ( A ) and GABA production ( B ) by Lv. brevis CRL 2013 in CDM or CDMg supplemented with different nitrogen sources. CDMg, chemically defined medium containing monosodium glutamate; CDMYE, CDM without glutamate supplemented with 1% yeast extract; CDMgYE, CDMg supplemented with 1% yeast extract; CDM Casitone 2%, CDM without glutamate supplemented with 2% Casitone; CDMgC0.5, CDMg supplemented with 0.5% Casitone; CDMgC, CDMg supplemented with 1%; CDMgC2, CDMg supplemented with 2% Casitone; CDMgC5, CDMg supplemented with 5% Casitone; CDMgVP, CDMg supplemented with 1% vegetable peptone. The pH profile observed in CDMgC0.5 was similar to those obtained in CDMg supplemented with 1% tryptone and 1% casamino acids, none of which supported detectable GABA production. Data are expressed as means ± SD from at least three independent biological replicates.

Article Snippet: To evaluate the role of nitrogen sources on GABA production, CDM containing 5% ( w / v ) monosodium glutamate (CDMg) as precursor was supplemented with different nitrogen sources ( ); 1% ( w / v ) yeast extract (Difco Laboratories, Sparks, MD, USA, CDMgYE), Casitone (Difco, 0.5, 1, 2, or 5% w / v ; CDMgC0.5, CDMgC, CDMC2 and CDMC5, respectively), Casamino Acids (Difco 1% w / v ; CDMgCA), Tryptone (Difco, 1% w / v ; CDMgT), or vegetable peptone (Difco, 1% w / v ; CDMgVP).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production in Levilactobacillus brevis CRL 2013

doi: 10.3390/ijms27010082

Figure Lengend Snippet: Differential expression of shared proteins between CDMgYE and CDMgC relative to CDMg. From the 55 proteins overexpressed in both CDMgYE and CDMgC relative to CDMg, the 20 most strongly upregulated in CDMgYE were selected (left blue panel). Their corresponding expression levels in Casitone (CDMgC) are shown for comparison (right green panel). Proteins are ranked according to their mean expression differences (log2) in CDMgYE. The most overexpressed was GadB ( Q03U69 , glutamate decarboxylase), directly involved in GABA synthesis. Bars represent mean log2 expression differences from three independent biological replicates.

Article Snippet: To evaluate the role of nitrogen sources on GABA production, CDM containing 5% ( w / v ) monosodium glutamate (CDMg) as precursor was supplemented with different nitrogen sources ( ); 1% ( w / v ) yeast extract (Difco Laboratories, Sparks, MD, USA, CDMgYE), Casitone (Difco, 0.5, 1, 2, or 5% w / v ; CDMgC0.5, CDMgC, CDMC2 and CDMC5, respectively), Casamino Acids (Difco 1% w / v ; CDMgCA), Tryptone (Difco, 1% w / v ; CDMgT), or vegetable peptone (Difco, 1% w / v ; CDMgVP).

Techniques: Quantitative Proteomics, Expressing, Comparison