casitone Search Results


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Thermo Fisher macrocystis pyrifera kelp casein enzymatic digest
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Thermo Fisher casein hydrolysate usb cspd appliedbiosystems nitro block
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MiddleBrook Pharmaceuticals mtb mic middlebrook 7h9/glucose/bsa/tx
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Mtb Mic Middlebrook 7h9/Glucose/Bsa/Tx, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioLife Solutions casitone
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Casitone, supplied by BioLife Solutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson bactotm casitone medium
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Bactotm Casitone Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson casitone
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Casitone, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HiMedia Laboratories casitone
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Casitone, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casitone/casitone/pmc06367689-66-0-7
Average 90 stars, based on 1 article reviews
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Becton Dickinson bacto-casitone
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Bacto Casitone, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Anaerobe Systems Inc yeast casitone fatty acid (ycfa, anaerobe system inc., morgan hill, ca, usa)
Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as <t>−log10(</t> <t>Mtb</t> <t>MIC</t> 99 in M).
Yeast Casitone Fatty Acid (Ycfa, Anaerobe System Inc., Morgan Hill, Ca, Usa), supplied by Anaerobe Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as −log10( Mtb MIC 99 in M).

Journal: ACS Infectious Diseases

Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides Target HadAB/BC Complex in Mycobacterium tuberculosis

doi: 10.1021/acsinfecdis.2c00392

Figure Lengend Snippet: Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as −log10( Mtb MIC 99 in M).

Article Snippet: Mtb MIC (Middlebrook 7H9/glucose/BSA/Tx) , 1.2 , 4.7.

Techniques: Inhibition, Binding Assay, Comparison, Activity Assay

Effect of 1,3-diarylpyrazolyl-acylsulfonamides on mycolic acid biosynthesis in Mycobacterium tuberculosis . Mtb H37Rv mc 2 6206 grown in Middlebrook 7H9-OADC-tyloxapol supplemented with casamino acids, pantothenate, and l -leucine at 37 °C was treated with no drug or 2 or 13 (1 to 6× MIC) and metabolically labeled with [1,2- 14 C]-acetate for 24 h. The same volume of [ 14 C]-acetate-labeled fatty acid and mycolic acid methyl esters (FAMEs and MAMEs) from treated and untreated cells were analyzed by TLC in the solvent system [ n -hexanes/ethyl acetate 95:5; by vol.; three developments] and revealed by PhosphorImaging. Compound 2 , a potent HadAB inhibitor, inhibited biosynthesis of mycolic acids up to 86% at 3× MIC in 24 h, whereas only 0–49% inhibition was observed with a weaker HadAB inhibitor 13 at 3× MIC.

Journal: ACS Infectious Diseases

Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides Target HadAB/BC Complex in Mycobacterium tuberculosis

doi: 10.1021/acsinfecdis.2c00392

Figure Lengend Snippet: Effect of 1,3-diarylpyrazolyl-acylsulfonamides on mycolic acid biosynthesis in Mycobacterium tuberculosis . Mtb H37Rv mc 2 6206 grown in Middlebrook 7H9-OADC-tyloxapol supplemented with casamino acids, pantothenate, and l -leucine at 37 °C was treated with no drug or 2 or 13 (1 to 6× MIC) and metabolically labeled with [1,2- 14 C]-acetate for 24 h. The same volume of [ 14 C]-acetate-labeled fatty acid and mycolic acid methyl esters (FAMEs and MAMEs) from treated and untreated cells were analyzed by TLC in the solvent system [ n -hexanes/ethyl acetate 95:5; by vol.; three developments] and revealed by PhosphorImaging. Compound 2 , a potent HadAB inhibitor, inhibited biosynthesis of mycolic acids up to 86% at 3× MIC in 24 h, whereas only 0–49% inhibition was observed with a weaker HadAB inhibitor 13 at 3× MIC.

Article Snippet: Mtb MIC (Middlebrook 7H9/glucose/BSA/Tx) , 1.2 , 4.7.

Techniques: Metabolic Labelling, Labeling, Solvent, Inhibition

 Mtb  Whole-Cell Activities of 1 and 2

Journal: ACS Infectious Diseases

Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides Target HadAB/BC Complex in Mycobacterium tuberculosis

doi: 10.1021/acsinfecdis.2c00392

Figure Lengend Snippet: Mtb Whole-Cell Activities of 1 and 2

Article Snippet: Mtb MIC (Middlebrook 7H9/glucose/BSA/Tx) , 1.2 , 4.7.

Techniques: Solubility