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Image Search Results
Journal: ACS Infectious Diseases
Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides Target HadAB/BC Complex in Mycobacterium tuberculosis
doi: 10.1021/acsinfecdis.2c00392
Figure Lengend Snippet: Chemoproteomic profiling of 1 and 2 identified HadA, HadB, and BCG_0547c (HadD) as targets. (A) Compounds 2 (blue) or 1 (red) were analyzed in a Mycobacterium bovis BCG protein extract with compound 3 and 4 attached to a bead matrix. Only three proteins showed competition by both compounds, HadA, HadB, and BCG_0547c (HadD). (B) Compound 1 (red) showed stronger inhibition of all three proteins from bead-binding in comparison to compound 2 (blue), indicating higher potency ( n = 3). (C) Correlation of target binding and antimycobacterial activity of compounds from the 1,3-diarylpyrazolyl-acylsulfonamide series. Active compounds ( 1 - 5 ) showed inhibition of proteins HadA, HadB, and BCG_0547c (HadD) from 4 -bead-binding, whereas inactive compounds ( 6 – 8 ) did not (results on 3 -beads are comparable, Figure S1 ). Numbers indicate compounds. r , Pearson correlation coefficient; p , p -value (calculated probability); pMIC99 defined as −log10( Mtb MIC 99 in M).
Article Snippet:
Techniques: Inhibition, Binding Assay, Comparison, Activity Assay
Journal: ACS Infectious Diseases
Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides Target HadAB/BC Complex in Mycobacterium tuberculosis
doi: 10.1021/acsinfecdis.2c00392
Figure Lengend Snippet: Effect of 1,3-diarylpyrazolyl-acylsulfonamides on mycolic acid biosynthesis in Mycobacterium tuberculosis . Mtb H37Rv mc 2 6206 grown in Middlebrook 7H9-OADC-tyloxapol supplemented with casamino acids, pantothenate, and l -leucine at 37 °C was treated with no drug or 2 or 13 (1 to 6× MIC) and metabolically labeled with [1,2- 14 C]-acetate for 24 h. The same volume of [ 14 C]-acetate-labeled fatty acid and mycolic acid methyl esters (FAMEs and MAMEs) from treated and untreated cells were analyzed by TLC in the solvent system [ n -hexanes/ethyl acetate 95:5; by vol.; three developments] and revealed by PhosphorImaging. Compound 2 , a potent HadAB inhibitor, inhibited biosynthesis of mycolic acids up to 86% at 3× MIC in 24 h, whereas only 0–49% inhibition was observed with a weaker HadAB inhibitor 13 at 3× MIC.
Article Snippet:
Techniques: Metabolic Labelling, Labeling, Solvent, Inhibition
Journal: ACS Infectious Diseases
Article Title: 1,3-Diarylpyrazolyl-acylsulfonamides Target HadAB/BC Complex in Mycobacterium tuberculosis
doi: 10.1021/acsinfecdis.2c00392
Figure Lengend Snippet: Mtb Whole-Cell Activities of 1 and 2
Article Snippet:
Techniques: Solubility