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anti camk2b  (Proteintech)


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    Structured Review

    Proteintech anti camk2b
    Anti Camk2b, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/camkii/CaMKII+beta+Antibody/pm42757697-65-11-15
    Average 93 stars, based on 13 article reviews
    anti camk2b - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Mechanical stretch-mediated fibroblast activation: The pivotal role of Piezo1 channels.
    Article Snippet: Then, it was fixed with 4 % paraformaldehyde (Solarbio, P1110) for 20 min. After three times PBS (Solarbio, P1010) washes, 1 mL of 0.2 % Triton X-100 (Solarbio, T8200) was added for 10 min. .. Following another set of PBS washes, 1 mL blocking solution (Solarbio, SW3015) was added, and cells were incubated at 37 ◦C for 1 h. Subsequently, 600 μL of primary antibodies Ki67 (1:250, Abcam, Cambridge, UK, ab15580), Piezo1 (1:200, SAB, Maryland, USA,30549), YAP1 (1:200, Proteintech, Wuhan, China, 13584-1-AP), α-SMA (1:200, Immunoway, USA, CA, YM3364), CaMKII (1:250, Proteintech, 12666-2-AP), or Phalloidin-iFluor® 488 Conjugate (AAT Bioquest, California, USA,23115) were added and incubated at 4 ◦C for 2 h. After three PBS washes, 600 μL Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (1:500, SAB, L35365) or Goat Anti-Rabbit IgG H&L (Alexa Flu-or® 488) (1:500, SAB, L35193) was added and incubated at 37 ◦C in the dark for 1 h. Following another three PBS washes. ..

    Incubation:

    Article Title: Mechanical stretch-mediated fibroblast activation: The pivotal role of Piezo1 channels.
    Article Snippet: Then, it was fixed with 4 % paraformaldehyde (Solarbio, P1110) for 20 min. After three times PBS (Solarbio, P1010) washes, 1 mL of 0.2 % Triton X-100 (Solarbio, T8200) was added for 10 min. .. Following another set of PBS washes, 1 mL blocking solution (Solarbio, SW3015) was added, and cells were incubated at 37 ◦C for 1 h. Subsequently, 600 μL of primary antibodies Ki67 (1:250, Abcam, Cambridge, UK, ab15580), Piezo1 (1:200, SAB, Maryland, USA,30549), YAP1 (1:200, Proteintech, Wuhan, China, 13584-1-AP), α-SMA (1:200, Immunoway, USA, CA, YM3364), CaMKII (1:250, Proteintech, 12666-2-AP), or Phalloidin-iFluor® 488 Conjugate (AAT Bioquest, California, USA,23115) were added and incubated at 4 ◦C for 2 h. After three PBS washes, 600 μL Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (1:500, SAB, L35365) or Goat Anti-Rabbit IgG H&L (Alexa Flu-or® 488) (1:500, SAB, L35193) was added and incubated at 37 ◦C in the dark for 1 h. Following another three PBS washes. ..

    Article Title: Vericiguat Alleviates Atrial Electrical and Structural Remodeling in Rats with Atrial Fibrillation
    Article Snippet: Proteins were then transferred to PVDF membranes (Millipore, USA) at 200 mA for 90 minutes. .. Membranes were blocked with a protein-free rapid sealing solution (PS108, Epizyme) and incubated overnight at 4°C with primary antibodies against: Collagen I (1:500, AF7001, Affinity), CaMKII (1:4000, 12666-2-AP, Proteintech), Cx43 (1:4000, 26980-1-AP, Proteintech), ATG7 (1:1000, ET1610-53, HuaAn), P62 (1:1000, A7785, ABclonal), LC3B (1:2000, ab192890, Abcam), GAPDH (1:30,000, AC001, ABclonal), and Tubulin (1:3000, 11224-1- AP; Proteintech). .. After washing with Tris-buffered saline with Tween 20, membranes were incubated with horseradish peroxidase–conjugated goat anti-rabbit IgG (1:10,000, bs-0295G-HRP, Bioss) for 1 hour at room temperature.

    Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress‐Induced Depression in Male Mice
    Article Snippet: For paraffin sections, the brains were embedded in paraffin (Thermo, USA), and then serially sectioned into 5‐μm‐thick transverse slices. .. For immunofluorescence analysis, the 30‐μm‐thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA‐1 (GB12105, Servicebio), CaMKII (11533‐1‐AP, Proteintech), GAD67 (PA5‐21397, Invitrogen), c‐Fos (OB‐PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523‐1‐AP, Proteintech). .. The sections were then incubated with fluorescence‐conjugated secondary antibodies at room temperature for 4 h. All sections were stained with DAPI for 10 min. For cell death detection, the 5‐μm‐thick sections were stained using the TUNEL kit (A112, Vazyme).

    Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress-Induced Depression in Male Mice.
    Article Snippet: For paraffin sections, the brains were embedded in paraffin (Thermo, USA), and then serially sectioned into 5-μm-thick transverse slices. .. For immunofluorescence analysis, the 30-μm-thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA-1 (GB12105, Servicebio), CaMKII (11533-1-AP, Proteintech), GAD67 (PA5-21397, Invitrogen), c-Fos (OB-PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523-1-AP, Proteintech). .. The sections were then incubated with fluorescence-conjugated secondary antibodies at room temperature for 4 h. All sections were stained with DAPI for 10 min. For cell death detection, the 5-μm-thick sections were stained using the TUNEL kit (A112, Vazyme).

    Article Title: α-Conotoxin LvID, an antagonist of α7 nicotinic acetylcholine receptor, mitigates Alzheimer-associated phenotypes by inhibiting Aβ deposition and reactive astrogliosis.
    Article Snippet: Alzheimer's disease (AD) is the most common form of dementia in older adults.. Neuritic plaques and reactive astrogliosis are neuropathological hallmarks of AD.. Amyloid beta (Aβ), the major component of neuritic plaques, is derived from amyloid precursor protein (APP) by sequential cleavages by β-secretase and γ-secretase.

    Immunofluorescence:

    Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress‐Induced Depression in Male Mice
    Article Snippet: For paraffin sections, the brains were embedded in paraffin (Thermo, USA), and then serially sectioned into 5‐μm‐thick transverse slices. .. For immunofluorescence analysis, the 30‐μm‐thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA‐1 (GB12105, Servicebio), CaMKII (11533‐1‐AP, Proteintech), GAD67 (PA5‐21397, Invitrogen), c‐Fos (OB‐PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523‐1‐AP, Proteintech). .. The sections were then incubated with fluorescence‐conjugated secondary antibodies at room temperature for 4 h. All sections were stained with DAPI for 10 min. For cell death detection, the 5‐μm‐thick sections were stained using the TUNEL kit (A112, Vazyme).

    Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress-Induced Depression in Male Mice.
    Article Snippet: For paraffin sections, the brains were embedded in paraffin (Thermo, USA), and then serially sectioned into 5-μm-thick transverse slices. .. For immunofluorescence analysis, the 30-μm-thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA-1 (GB12105, Servicebio), CaMKII (11533-1-AP, Proteintech), GAD67 (PA5-21397, Invitrogen), c-Fos (OB-PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523-1-AP, Proteintech). .. The sections were then incubated with fluorescence-conjugated secondary antibodies at room temperature for 4 h. All sections were stained with DAPI for 10 min. For cell death detection, the 5-μm-thick sections were stained using the TUNEL kit (A112, Vazyme).

    Saline:

    Article Title: α-Conotoxin LvID, an antagonist of α7 nicotinic acetylcholine receptor, mitigates Alzheimer-associated phenotypes by inhibiting Aβ deposition and reactive astrogliosis.
    Article Snippet: Alzheimer's disease (AD) is the most common form of dementia in older adults.. Neuritic plaques and reactive astrogliosis are neuropathological hallmarks of AD.. Amyloid beta (Aβ), the major component of neuritic plaques, is derived from amyloid precursor protein (APP) by sequential cleavages by β-secretase and γ-secretase.



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    Image Search Results


    A Representative western blotting images showing the protein expression of p-CaMKII (Thr286) in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.

    Journal: Cell Death & Disease

    Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission

    doi: 10.1038/s41419-026-09084-3

    Figure Lengend Snippet: A Representative western blotting images showing the protein expression of p-CaMKII (Thr286) in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.

    Article Snippet: Anti-p-CaMKII (Thr286) , 12716 , Rabbit , CST , WB (1:1000).

    Techniques: Western Blot, Expressing, Incubation, Immunofluorescence, Staining, Morphology, CCK-8 Assay, Control

    List of primary antibodies, secondary antibodies, and nuclear dyes used in this study.

    Journal: Cell Death & Disease

    Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission

    doi: 10.1038/s41419-026-09084-3

    Figure Lengend Snippet: List of primary antibodies, secondary antibodies, and nuclear dyes used in this study.

    Article Snippet: Anti-p-CaMKII (Thr286) , 12716 , Rabbit , CST , WB (1:1000).

    Techniques: