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mouse anti calbindin d28k  (Proteintech)


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    Structured Review

    Proteintech mouse anti calbindin d28k
    Mouse Anti Calbindin D28k, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calbindin/Calbindin-D28k+Monoclonal+antibody/pm42768773-361-21-25
    Average 93 stars, based on 18 article reviews
    mouse anti calbindin d28k - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Purification:

    Article Title: Effect of conditional deletion of cytoplasmic dynein heavy chain DYNC1H1 on postnatal photoreceptors
    Article Snippet: .. Antibodies, dilutions and sources follow: DYNC1H1 (dilution 1:250, Proteintech 12345-1-AP) [ , ]; rhodopsin (1:1000, 4D2 Abcam 98887); PDE6 (1:1000, MOE Cytosignal) [ ]; OPN1SW (1:500, Millipore Sigma AB5407) [ , ]; OPN1MW/OPN1LW (1:500, Millipore Sigma AB5405) [ , ]; cone arrestin (ARR3, 1:250, outsourced to Covance, Princeton NJ and purified in house) [ ]; CtBP2/Ribeye (1:10,000, BD Biosciences 612044); Calbindin (1:200, Proteintech 14479-1-AP); PKCα (1:150, Cell Signaling 20565); monoclonal anti-Glutamine Synthetase (GluSyn), BD Biosciences 610518). ..

    Immunofluorescence:

    Article Title: Clock knockout in inhibitory neurons reduces predisposition to epilepsy and influences anxiety-like behaviors in mice.
    Article Snippet: .. For immunofluorescence staining, the tissue sections were incubated with PBS containing 0.2% Triton X-100 (v/v) for 20 min, incubated in blocking buffer (10% normal goat serum [v/v] in PBS) for 1 h at room temperature, and then in primary antibodies against parvalbumin (1:200, P3088, Sigma), CLOCK (1:100, ab3517, Abcam), Calretinin (1:500, 66496-1-Ig,Proteintech) Calbindin (1:500, 66394-1-Ig, Proteintech), GFAP (1:500, 3670S, Cell Signaling Technology), NeuN (1:200,12,943 s, Abcam) diluted in 1× PBS + 10% normal goat serum (v/v) at 4 ◦C overnight. .. The next day, the sections were washed three times with 1× PBS and incubated in appropriate secondary antibodies for 2 h at room temperature: Alexa Fluor-488 goat anti-rabbit IgG (1:500; AB150077; Abcam), Alexa Fluor-555 goat antirabbit IgG (1:200; AB150078; Abcam), M5 goat anti-mouse IgG, Cy3conjugated (1:200, MF251; Mei5bio), and M5 goat anti-mouse IgG, FITC-conjugated (1:500, MF201, Mei5bio).

    Article Title: Generation of renal tubular organoids from adult SOX9 + kidney progenitor cells
    Article Snippet: .. Antibodies used for immunofluorescence include: SOX9 (1:200, CY5400, Abways), Ki67 (1:100, 550609, BD bioscience), ATP1A1 (1:50, sc-514614, Santa cruz), AQP1 (1:50, sc-25287, Santa cruz), SLC22A6 (1:200, ab183086, Abcam), Umod (1:200, ab207170, Abcam), AQP2 (1:200, ab199975, Abcam), Calbindin (1:200, 14479-1-AP, Proteintech), SLC12A3 (1:200, ab95302, Abcam), PAX8 (1:200, ab189249, Abcam), SYNPO (1:200, 21064-1-AP, Proteintech), GFP (1:200, ab290, Abcam), GFP (1:500, ab6673, Abcam), HuNu (1:200, ab191181, Abcam), and human specific Lamin A+C (1:200, ab108595, Abcam). .. Alexa Fluor-conjugated Donkey 488/594 (1:200, Life Technologies, USA) were used as secondary antibodies.

    Article Title: Generation of renal tubular organoids from adult SOX9+ kidney progenitor cells
    Article Snippet: .. Antibodies used for immunofluorescence include: SOX9 (1:200, CY5400, Abways), Ki67 (1:100, 550609, BD bioscience), ATP1A1 (1:50, sc-514614, Santa cruz), AQP1 (1:50, sc-25287, Santa cruz), SLC22A6 (1:200, ab183086, Abcam), Umod (1:200, ab207170, Abcam), AQP2 (1:200, ab199975, Abcam), Calbindin (1:200, 14479-1-AP, Proteintech), SLC12A3 (1:200, ab95302, Abcam), PAX8 (1:200, ab189249, Abcam), SYNPO (1:200, 21064-1-AP, Proteintech), GFP (1:200, ab290, Abcam), GFP (1:500, ab6673, Abcam), HuNu (1:200, ab191181, Abcam), and human specific Lamin A+C (1:200, ab108595, Abcam). .. Alexa Fluor-conjugated Donkey 488/594 (1:200, Life Technologies, USA) were used as secondary antibodies.

    Staining:

    Article Title: Clock knockout in inhibitory neurons reduces predisposition to epilepsy and influences anxiety-like behaviors in mice.
    Article Snippet: .. For immunofluorescence staining, the tissue sections were incubated with PBS containing 0.2% Triton X-100 (v/v) for 20 min, incubated in blocking buffer (10% normal goat serum [v/v] in PBS) for 1 h at room temperature, and then in primary antibodies against parvalbumin (1:200, P3088, Sigma), CLOCK (1:100, ab3517, Abcam), Calretinin (1:500, 66496-1-Ig,Proteintech) Calbindin (1:500, 66394-1-Ig, Proteintech), GFAP (1:500, 3670S, Cell Signaling Technology), NeuN (1:200,12,943 s, Abcam) diluted in 1× PBS + 10% normal goat serum (v/v) at 4 ◦C overnight. .. The next day, the sections were washed three times with 1× PBS and incubated in appropriate secondary antibodies for 2 h at room temperature: Alexa Fluor-488 goat anti-rabbit IgG (1:500; AB150077; Abcam), Alexa Fluor-555 goat antirabbit IgG (1:200; AB150078; Abcam), M5 goat anti-mouse IgG, Cy3conjugated (1:200, MF251; Mei5bio), and M5 goat anti-mouse IgG, FITC-conjugated (1:500, MF201, Mei5bio).

    Incubation:

    Article Title: Clock knockout in inhibitory neurons reduces predisposition to epilepsy and influences anxiety-like behaviors in mice.
    Article Snippet: .. For immunofluorescence staining, the tissue sections were incubated with PBS containing 0.2% Triton X-100 (v/v) for 20 min, incubated in blocking buffer (10% normal goat serum [v/v] in PBS) for 1 h at room temperature, and then in primary antibodies against parvalbumin (1:200, P3088, Sigma), CLOCK (1:100, ab3517, Abcam), Calretinin (1:500, 66496-1-Ig,Proteintech) Calbindin (1:500, 66394-1-Ig, Proteintech), GFAP (1:500, 3670S, Cell Signaling Technology), NeuN (1:200,12,943 s, Abcam) diluted in 1× PBS + 10% normal goat serum (v/v) at 4 ◦C overnight. .. The next day, the sections were washed three times with 1× PBS and incubated in appropriate secondary antibodies for 2 h at room temperature: Alexa Fluor-488 goat anti-rabbit IgG (1:500; AB150077; Abcam), Alexa Fluor-555 goat antirabbit IgG (1:200; AB150078; Abcam), M5 goat anti-mouse IgG, Cy3conjugated (1:200, MF251; Mei5bio), and M5 goat anti-mouse IgG, FITC-conjugated (1:500, MF201, Mei5bio).

    Blocking Assay:

    Article Title: Clock knockout in inhibitory neurons reduces predisposition to epilepsy and influences anxiety-like behaviors in mice.
    Article Snippet: .. For immunofluorescence staining, the tissue sections were incubated with PBS containing 0.2% Triton X-100 (v/v) for 20 min, incubated in blocking buffer (10% normal goat serum [v/v] in PBS) for 1 h at room temperature, and then in primary antibodies against parvalbumin (1:200, P3088, Sigma), CLOCK (1:100, ab3517, Abcam), Calretinin (1:500, 66496-1-Ig,Proteintech) Calbindin (1:500, 66394-1-Ig, Proteintech), GFAP (1:500, 3670S, Cell Signaling Technology), NeuN (1:200,12,943 s, Abcam) diluted in 1× PBS + 10% normal goat serum (v/v) at 4 ◦C overnight. .. The next day, the sections were washed three times with 1× PBS and incubated in appropriate secondary antibodies for 2 h at room temperature: Alexa Fluor-488 goat anti-rabbit IgG (1:500; AB150077; Abcam), Alexa Fluor-555 goat antirabbit IgG (1:200; AB150078; Abcam), M5 goat anti-mouse IgG, Cy3conjugated (1:200, MF251; Mei5bio), and M5 goat anti-mouse IgG, FITC-conjugated (1:500, MF201, Mei5bio).

    other:

    Article Title: RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation
    Article Snippet: BDNF (1:1000; Abcam, ab108319), FLAG (1:2500; Sigma–Aldrich, F1804), GAPDH (1:3000; ProteinTech, 60004-1-Ig), PTBP1 (1:1000; Abcam, ab133734), PTBP2 (1:1000; Abcam, ab228930), Lamin A/C (1:3000; Sigma–Aldrich, SAB4200236), Calbindin (1:200; Proteintech, 14479-1-AP), Pax6 (1:150; Millipore, AB2237), Akt (1:2000; Cell Signaling Technology, 9272), pAkt (1:1000; Cell Signaling Technology, 9271), ERK1/2 (1:2000; Cell Signaling Technology, 9102), pERK1/2 (1:1000; Cell Signaling Technology, 9101), CC3 (1:1000; Cell Signaling Technology, 9664), pCREB (1:1000; Proteintech, 28792-1-AP).

    Article Title: RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation.
    Article Snippet: BDNF (1:1000; Abcam, ab108319), FLAG (1:2500; Sigma–Aldrich, F1804), GAPDH (1:3000; ProteinTech, 60004-1-Ig), PTBP1 (1:1000; Abcam, ab133734), PTBP2 (1:1000; Abcam, ab228930), Lamin A/C (1:3000; Sigma–Aldrich, SAB4200236), Calbindin (1:200; Proteintech, 14479-1-AP), Pax6 (1:150; Millipore, AB2237), Akt (1:2000; Cell Signaling Technology, 9272), pAkt (1:1000; Cell Signaling Technology, 9271), ERK1/2 (1:2000;Cell SignalingTechnology, 9102), pERK1/2 (1:1000; Cell Signaling Technology, 9101), CC3 (1:1000; Cell Signaling Technology, 9664), pCREB (1:1000; Proteintech, 28792-1-AP).



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    Image Search Results


    (A) Calbindin-labeled inferior olive somata with little intervening space (arrowheads). (B) PDX1-Cre × Ai9 dataset and soma-center map; orange points indicate somata with close neighbors. (C) Top, soma-equivalent diameters overlap the distribution of nearest-neighbor center distances, indicating that many somata lie close enough to contact. Bottom, close-neighbor prevalence was compared with packing-aware shuffled null datasets for each slice (S1–S8). Colored points show the prevalence measured from the real soma positions, and matching colored curves show the cumulative distributions obtained after shuffling soma positions within each slice footprint. The rightward displacement of real values relative to the shuffled distributions indicates that close somatic neighbors occur more often than expected from packing constraints alone. Scale bars: A, 50 µ m; B, 200 µ m.

    Journal: bioRxiv

    Article Title: Somatic Cx36 gap junctions expand the anatomical substrate for electrical coupling in the adult mouse inferior olive

    doi: 10.64898/2026.09.09.750283

    Figure Lengend Snippet: (A) Calbindin-labeled inferior olive somata with little intervening space (arrowheads). (B) PDX1-Cre × Ai9 dataset and soma-center map; orange points indicate somata with close neighbors. (C) Top, soma-equivalent diameters overlap the distribution of nearest-neighbor center distances, indicating that many somata lie close enough to contact. Bottom, close-neighbor prevalence was compared with packing-aware shuffled null datasets for each slice (S1–S8). Colored points show the prevalence measured from the real soma positions, and matching colored curves show the cumulative distributions obtained after shuffling soma positions within each slice footprint. The rightward displacement of real values relative to the shuffled distributions indicates that close somatic neighbors occur more often than expected from packing constraints alone. Scale bars: A, 50 µ m; B, 200 µ m.

    Article Snippet: Sections were incubated at 4°C for 48 h in antibody buffer (2% BSA and 0.4% Triton X-100 in PBS) containing mouse anti-Cx36 primary antibody (1:200 dilution, 2.5 μ g/mL, #37-4600, Thermo Fisher Invitrogen) and guinea pig anti-calbindin antibody (1 μ g/mL, #214 005, Synaptic Systems), with shaking at 80 rpm.

    Techniques: Labeling

    Confocal images of Calbindin-labeled olivary somata and Cx36 immunolabeling from multiple examples of closely apposed somata. Images are maximum-intensity projections of optical sections acquired at 0.1 µ m z-steps, with each projected substack spanning less than 2 µ m in z . For each example, single-channel Calbindin and Cx36 panels show the cellular context and punctate Cx36 signal, and the merged panels show Cx36 puncta concentrated at or immediately adjacent to the soma-soma interface. The repeated occurrence of puncta at close somatic contacts supports the presence of Cx36-containing junctional specializations beyond dendritic spines. Scale bars: 5 µ m.

    Journal: bioRxiv

    Article Title: Somatic Cx36 gap junctions expand the anatomical substrate for electrical coupling in the adult mouse inferior olive

    doi: 10.64898/2026.09.09.750283

    Figure Lengend Snippet: Confocal images of Calbindin-labeled olivary somata and Cx36 immunolabeling from multiple examples of closely apposed somata. Images are maximum-intensity projections of optical sections acquired at 0.1 µ m z-steps, with each projected substack spanning less than 2 µ m in z . For each example, single-channel Calbindin and Cx36 panels show the cellular context and punctate Cx36 signal, and the merged panels show Cx36 puncta concentrated at or immediately adjacent to the soma-soma interface. The repeated occurrence of puncta at close somatic contacts supports the presence of Cx36-containing junctional specializations beyond dendritic spines. Scale bars: 5 µ m.

    Article Snippet: Sections were incubated at 4°C for 48 h in antibody buffer (2% BSA and 0.4% Triton X-100 in PBS) containing mouse anti-Cx36 primary antibody (1:200 dilution, 2.5 μ g/mL, #37-4600, Thermo Fisher Invitrogen) and guinea pig anti-calbindin antibody (1 μ g/mL, #214 005, Synaptic Systems), with shaking at 80 rpm.

    Techniques: Confocal, Labeling, Immunolabeling