Journal: eLife

Article Title: Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development

doi: 10.7554/eLife.78202

Figure Lengend Snippet: ( A ) Representative tiled confocal images of WT and Dp10 P4 animals corresponding to the same cerebellar folia in each animal. Brain sections were stained with Hoechst 33342 and the cell proliferation marker Ki67. ( B ) Quantitation of the number of Ki67-positive cells in the external granular layer of WT and Dp10 animals. Graph shows mean ± SD. N=3. Mann-Whitney U test. ( C ) Representative tiled confocal images of WT and Dp10 P4 animals corresponding to the same cerebellar folia in each animal. Brain sections were stained with Hoechst 33342 and the Purkinje cell marker calbindin (CALB1) and the microtubule binding protein doublecortin (DCX). The far-right panel shows insets of DCX staining. ( D ) Quantitation of primary cilia frequency in the inner granule layer of WT and Dp10 animals normalized to WT. Graph shows mean ± SD. N=3. Wilcoxon matched-pairs test. ( E ) Representative tiled confocal images of the cerebellum from P21 wild-type (WT) and Dp10 animals. Brain sections were stained with Hoechst 33342, ARL13B, and γ-tubulin. Insets show zoomed in regions corresponding to the same folia in each animal. ( F ) Quantitation of primary cilia frequency in WT and Dp animals at P21 normalized to WT at P21. Graph shows mean ± SD. N=3. Wilcoxon matched-pairs test. ( G–H ) Quantitation of the granular layer ( G ) and molecular layer ( H ) widths in WT and Dp10 animals at P21. Graphs show mean ± SD. N=3. Mann-Whitney U test.

Article Snippet: Antibody , CALB1 (chicken) , Neuromics , Cat# CH22118, RRID: AB_2737107 , IF (1:1000).

Techniques: Staining, Marker, Quantitation Assay, MANN-WHITNEY, Binding Assay

Journal: eLife

Article Title: Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development

doi: 10.7554/eLife.78202

Figure Lengend Snippet:

Article Snippet: Antibody , CALB1 (chicken) , Neuromics , Cat# CH22118, RRID: AB_2737107 , IF (1:1000).

Techniques: Transduction, Marker, Transfection, Construct, Plasmid Preparation, Sequencing, Negative Control, Antibody Labeling

Journal: PLoS Genetics

Article Title: Rgs6 is Required for Adult Maintenance of Dopaminergic Neurons in the Ventral Substantia Nigra

doi: 10.1371/journal.pgen.1004863

Figure Lengend Snippet: (A) Rgs6 expression revealed by immunohistofluorescence (red) together with TH (green) in mDA neurons of SNc but not VTA. Scale bar 410 µm. (B) Co-immunofluorescence analysis of TH (green) and Rgs6 or Pitx3 (red, as indicated) in SNc and VTA of adult mice. Arrowheads point to Pitx3-negative or Rgs6-negative mDA neurons in dorsal SNc. Scale bar 100 µm. (C) Triple immunofluorescence staining for TH (green), Pitx3 (red) and Calb1 (blue) on tissue sections of adult mice indicates that the majority of TH+Pitx3− cells of dSNc (arrowheads) are positive for Calb1, while TH+Pitx3+ cells of vSNc are negative for Calb1, consistent with depiction in A. Scale bar 100 µm.

Article Snippet: Primary antibodies used were against Pitx3 (rabbit home-made, 1∶400), Rgs6 (rabbit home-made 1∶25), Th (Millipore MAB318, 1∶1000), Th (Millipore AB152, 1∶500), Calb1 (R&D Systems AF3320, 1∶40), Fgf10 (Millipore ABN44, 1∶1000), Slc6a3 (Santa Cruz sc-32258, 1∶250), Drd2 (Santa Cruz sc5303, 1∶100), Aldh1a1 (Abcam ab24343, 1∶400), BDNF (Abcam ab108319, 1∶25), phospho-p27 (Abcam ab32096, 1∶50), phospho-Erk1/2 (Cell Signaling 4376, 1∶25), DJ-1 (Abcam ab18257, 1∶500) Lrrk2 (Epitomics 3514-1, 1∶50), Pink1 (Novus Biologicals BC100-494, 1∶50), LC3B (Cell Signaling 3868, 1∶50), and Vmat2 (Millipore AB1598P, 1∶200).

Techniques: Expressing, Immunohistofluorescence, Immunofluorescence, Staining

Journal: eLife

Article Title: LKB1 coordinates neurite remodeling to drive synapse layer emergence in the outer retina

doi: 10.7554/eLife.56931

Figure Lengend Snippet: Antibodies used in LKB1 mutant tissue analysis. Antibodies were utilized that label individual neuron populations and synapses in the outer retina.

Article Snippet: Calbindin D-28k , Full-length recombinant human Calbindin D-28K , Horizontal cells; amacrine cells; retinal ganglion cells , Novus biologicals; chicken polyclonal; NBP2-50028; no RRID , 1:2000.

Techniques: Mutagenesis, Labeling, Concentration Assay, Recombinant, Derivative Assay, Purification

Journal: Endocrinology

Article Title: Exogenous 1,25-dihydroxyvitamin D3 exerts a skeletal anabolic effect and improves mineral ion homeostasis in mice that are homozygous for both the 1alpha-hydroxylase and parathyroid hormone null alleles.

doi: 10.1210/en.2006-0403

Figure Lengend Snippet: FIG. 1. Effect of exogenous 1,25(OH)2D3 on serum calcium and phosphorus, urine calcium relative to creatinine, and renal calcium transporters in vivo. Serum calcium (A) and phosphorus (B) and urine calcium to creatinine ratio (C) were determined in 2-wk-old vehicle-treated wild-type (WT-vehicle), vehicle-treated PTH/1(OH)ase/ mice (DKO-vehicle), and 1,25(OH)2D3-treated PTH/1(OH)ase/ mice (DKO1,25D) as described in Materials and Methods. Each value is the mean SEM of determinations in six mice of each group. D, Representative RT-PCR analysis of renal extracts for gene expression of TRPV5, calbindin-D9K (CB9K), calbindin-D28K (CB28K), and NCX1. Glyceraldehyde-3- phosphate dehydrogenase (GAPDH) mRNA was the loading control. E, Representative Western blots of renal extracts for expression of TRPV5, CB9K, CB28K, and NCX1. -Tubulin was used as a loading control for Western blots. F, TRPV5, CB28K, CB9,K and NCX1 mRNA levels relative to GAPDH mRNA level were assessed by densitometric analysis and expressed relative to respective levels of these transporters in wild-type mice. Each value is the mean SEM of triplicate determinations. G, TRPV5, CB28K, CB9K, and NCX1 protein levels relative to -tubulin protein level were assessed by densitometric analysis and expressed relative to respective levels of these transporters in wild-type mice. Each value is the mean SEM of triplicate determinations. *, P 0.05; ***, P 0.001, compared with vehicle-treated wild-type mice; #, P 0.05; ###, P 0.001, compared with vehicle-treated double-KO mice.

Article Snippet: Immunoblotting was carried out as described (24–26) using antibodies against TRPV5 (ECaC1), calbindin-D28K, and calbindin-D9K, Na /Ca2 exchanger 1 (NCX1) (Swant, Bellinzona, Switzerland) and -tubulin (Santa Cruz Biotechnology Inc., Santa Cruz, CA).

Techniques: In Vivo, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Control, Western Blot, Expressing