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caco  (ATCC)


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    ATCC caco
    Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 <t>in</t> <t>Caco-2</t> monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
    Caco, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 17051 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco/product/ATCC
    Average 99 stars, based on 17051 article reviews
    caco - by Bioz Stars, 2026-04
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    Images

    1) Product Images from "Harnessing the gut–immune–joint axis: Oral microalgae-based thermoresponsive microspheres enhance intra-articular therapy for rheumatoid arthritis"

    Article Title: Harnessing the gut–immune–joint axis: Oral microalgae-based thermoresponsive microspheres enhance intra-articular therapy for rheumatoid arthritis

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.01.037

    Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 in Caco-2 monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
    Figure Legend Snippet: Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 in Caco-2 monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

    Techniques Used: Disruption, In Vitro, Immunofluorescence, Staining, Fluorescence, Co-Culture Assay



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    Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 <t>in</t> <t>Caco-2</t> monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.
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    Effects of four Antrodia cinnamomea -derived triterpenoid compounds on CRC cell viability. Cell viability was assessed by Cell Counting Kit-8 assay in three human CRC cell lines: (A) HCT116 (KRAS mutant), (B) HT29 (KRAS wild-type) and <t>(C)</t> <t>Caco-2</t> (KRAS wild-type) after treatment with increasing concentrations (0.1, 1, 3 and 10 μ M) of DeEA, DSA, 4AAQB and LT4 for 24, 48 and 72 h. Absorbance was recorded at 450/650 nm and normalized to vehicle-treated controls (0.1% DMSO). Data are presented as the mean ± SD from at least three independent experiments (n≥3). * P<0.05, ** P<0.01, *** P<0.001 vs. vehicle-treated control. CRC, colorectal cancer; DeEA, dehydroeburicoic acid; DSA, dehydrosulphurenic acid; 4AAQB, 4-acetylantroquinonol B; LT4, 4-acetylantrocamol LT3.
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    Image Search Results


    Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 in Caco-2 monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

    Journal: Bioactive Materials

    Article Title: Harnessing the gut–immune–joint axis: Oral microalgae-based thermoresponsive microspheres enhance intra-articular therapy for rheumatoid arthritis

    doi: 10.1016/j.bioactmat.2026.01.037

    Figure Lengend Snippet: Evaluation of the protective effects of CG@GelMA on FSN-induced intestinal epithelial barrier disruption in vitro . (A) Immunofluorescence staining of Claudin-1 in Caco-2 monolayers under different treatments. Scale bar: 100 μm. (B) Relative fluorescence intensity of Claudin-1. (C) Immunofluorescence staining of Occludin in Caco-2 monolayers. Scale bar: 100 μm. (D) Relative fluorescence intensity of Occludin. (E) Immunofluorescence staining of ZO-1 in Caco-2 monolayers. Scale bar: 100 μm. (F) Relative fluorescence intensity of ZO-1. (G) Schematic diagram of the Caco-2/RAW 264.7 Transwell co-culture system. (H) Relative TEER of Caco-2 cell monolayers after different treatments. (I) Relative fluorescence intensity of FD4 across Caco-2 monolayers under different treatments. Data are presented as means ± SD. Statistical significance: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

    Article Snippet: RAW 264.7 macrophages (Procell, Wuhan, China), Caco-2 intestinal epithelial cells (Pricella, Wuhan, China), and IEC-6 small intestinal epithelial cells (ATCC, USA) were maintained in high-glucose DMEM supplemented with 10 % fetal bovine serum (AiTing, Hangzhou, China) and 1 % penicillin-streptomycin (Gibco, USA), with the medium for IEC-6 cells additionally containing 0.1 U/mL human insulin.

    Techniques: Disruption, In Vitro, Immunofluorescence, Staining, Fluorescence, Co-Culture Assay

    Effects of four Antrodia cinnamomea -derived triterpenoid compounds on CRC cell viability. Cell viability was assessed by Cell Counting Kit-8 assay in three human CRC cell lines: (A) HCT116 (KRAS mutant), (B) HT29 (KRAS wild-type) and (C) Caco-2 (KRAS wild-type) after treatment with increasing concentrations (0.1, 1, 3 and 10 μ M) of DeEA, DSA, 4AAQB and LT4 for 24, 48 and 72 h. Absorbance was recorded at 450/650 nm and normalized to vehicle-treated controls (0.1% DMSO). Data are presented as the mean ± SD from at least three independent experiments (n≥3). * P<0.05, ** P<0.01, *** P<0.001 vs. vehicle-treated control. CRC, colorectal cancer; DeEA, dehydroeburicoic acid; DSA, dehydrosulphurenic acid; 4AAQB, 4-acetylantroquinonol B; LT4, 4-acetylantrocamol LT3.

    Journal: International Journal of Molecular Medicine

    Article Title: 4-Acetylantrocamol LT3 suppresses colorectal cancer growth and metastasis via PI3K/AKT and MAPK pathway modulation

    doi: 10.3892/ijmm.2026.5797

    Figure Lengend Snippet: Effects of four Antrodia cinnamomea -derived triterpenoid compounds on CRC cell viability. Cell viability was assessed by Cell Counting Kit-8 assay in three human CRC cell lines: (A) HCT116 (KRAS mutant), (B) HT29 (KRAS wild-type) and (C) Caco-2 (KRAS wild-type) after treatment with increasing concentrations (0.1, 1, 3 and 10 μ M) of DeEA, DSA, 4AAQB and LT4 for 24, 48 and 72 h. Absorbance was recorded at 450/650 nm and normalized to vehicle-treated controls (0.1% DMSO). Data are presented as the mean ± SD from at least three independent experiments (n≥3). * P<0.05, ** P<0.01, *** P<0.001 vs. vehicle-treated control. CRC, colorectal cancer; DeEA, dehydroeburicoic acid; DSA, dehydrosulphurenic acid; 4AAQB, 4-acetylantroquinonol B; LT4, 4-acetylantrocamol LT3.

    Article Snippet: Human CRC cell lines, HCT116 (KRAS mutant), HT-29 and Caco-2, were purchased from the American Type Culture Collection (ATCC; HCT116, CCL-247; HT-29, HTB-38; Caco-2, HTB-37).

    Techniques: Derivative Assay, Cell Counting, Mutagenesis, Control