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bioswamp  (Bioss)


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    Structured Review

    Bioss bioswamp
    Bioswamp, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioswamp/product/Bioss
    Average 94 stars, based on 71 article reviews
    bioswamp - by Bioz Stars, 2026-04
    94/100 stars

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    In vitro activity assays of PDCoV Macro-Ubl2-PLpro and truncated protein. (A) PDCoV Macro-Ubl2-PLpro and truncated constructs. (B) The hydrolytic activity assay was performed with 2 µM enzyme and 40 µM FRET peptides. The Macro-Ubl2-PLpro fluorescence intensity value was set to 100%. (C) The deubiquitinating activity assay was determined with 1 µM enzyme and 0.4 µM Ub-AMC. (D) The deISGylating activity assay was determined with 1 µM enzyme and 0.4 µM ISG15-AMC. Experiments were performed in triplicate, the wild-type fluorescence intensity value was set to 100%. The error bars represent the standard deviations for a minimum of triplicate samples. Asterisks indicate statistical significance calculated using unpaired two-tailed student’s t -test, and values of 0.05 were considered statistically significant. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Emerging Microbes & Infections

    Article Title: Structure of the multiple functional domains from coronavirus nonstructural protein 3

    doi: 10.1080/22221751.2020.1865840

    Figure Lengend Snippet: In vitro activity assays of PDCoV Macro-Ubl2-PLpro and truncated protein. (A) PDCoV Macro-Ubl2-PLpro and truncated constructs. (B) The hydrolytic activity assay was performed with 2 µM enzyme and 40 µM FRET peptides. The Macro-Ubl2-PLpro fluorescence intensity value was set to 100%. (C) The deubiquitinating activity assay was determined with 1 µM enzyme and 0.4 µM Ub-AMC. (D) The deISGylating activity assay was determined with 1 µM enzyme and 0.4 µM ISG15-AMC. Experiments were performed in triplicate, the wild-type fluorescence intensity value was set to 100%. The error bars represent the standard deviations for a minimum of triplicate samples. Asterisks indicate statistical significance calculated using unpaired two-tailed student’s t -test, and values of 0.05 were considered statistically significant. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Antibodies used in this study were anti-ubiquitin (P4D1, Santa Cruz Biotech), anti-ISG15 (Bioswamp), anti-His (Proteintech), anti-K48-ubiquitin (Boster), goat anti-rabbit secondary antibodies (Proteintech), and goat anti-mouse IgG light-chain secondary antibodies (Proteintech).

    Techniques: In Vitro, Activity Assay, Construct, Fluorescence, Two Tailed Test