bioswamp Search Results


hm10669 human amh elisa kit bioswamp  (Multi Sciences (Lianke) Biotech Co Ltd)
94
Multi Sciences (Lianke) Biotech Co Ltd hm10669 human amh elisa kit bioswamp
Hm10669 Human Amh Elisa Kit Bioswamp, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hm10669 human amh elisa kit bioswamp/product/Multi Sciences (Lianke) Biotech Co Ltd
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anti lamc1  (Proteintech)
93
Proteintech anti lamc1
Anti Lamc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tsg101  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc tsg101
Tsg101, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lc3b  (Proteintech)
96
Proteintech lc3b
Lc3b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bax  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc bax
Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gclc  (Proteintech)
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Proteintech anti gclc
Anti Gclc, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glyceraldehyde 3 phosphate dehydrogenase  (Proteintech)
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Proteintech glyceraldehyde 3 phosphate dehydrogenase
Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti zo 1  (Proteintech)
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Proteintech anti zo 1
Anti Zo 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against caspase 3  (Sanying Ltd)
90
Sanying Ltd primary antibodies against caspase 3
Primary Antibodies Against Caspase 3, supplied by Sanying Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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slc27a2  (Proteintech)
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Proteintech slc27a2
Slc27a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrp1  (Proteintech)
93
Proteintech mrp1
FEZF1-AS1 was upregulated in chemo-resistant GC tissues and knockdown of FEZF1-AS1 improved chemo-sensitivity of GC cells. (A) qRT-PCR on the level of FEZF1-AS1 in 27 pairs of specimens from GC patients with poor response to chemotherapy were 3.5-fold increase compared with patients with good response. (B) CCK8 assay on the viability of SGC-7901 and SGC-7901/CDDP cells treated with 10–80 μg/ml of CDDP for 24 h. (C) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/CDDP cells. (D) CCK8 assay on the viability of SGC-7901 and SGC-7901/5-FU cells treated with 5–25 μg/ml of 5-FU for 24 h. (E) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/5-FU cells. (F) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 mg/ml). (G) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 μg/ml). (H) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). (I) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). The protein expression of MDR1 and <t>MRP1</t> in SGC-7901/CDDP (J) and SGC-7901/5-FU (L) cells detected by Western blot, FEZF1-AS1 siRNA decreased the protein levels of MDR1 and MRP1 in both cells. The knockdown efficiency of FEZF1-AS1 in SGC-7901/CDDP (K) and SGC-7901/5-FU (M) cells detected by qRT-PCR. Each experiment was performed in triplicate. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Mrp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyp11a1 pab40042 bioswamp  (Proteintech)
95
Proteintech cyp11a1 pab40042 bioswamp
FEZF1-AS1 was upregulated in chemo-resistant GC tissues and knockdown of FEZF1-AS1 improved chemo-sensitivity of GC cells. (A) qRT-PCR on the level of FEZF1-AS1 in 27 pairs of specimens from GC patients with poor response to chemotherapy were 3.5-fold increase compared with patients with good response. (B) CCK8 assay on the viability of SGC-7901 and SGC-7901/CDDP cells treated with 10–80 μg/ml of CDDP for 24 h. (C) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/CDDP cells. (D) CCK8 assay on the viability of SGC-7901 and SGC-7901/5-FU cells treated with 5–25 μg/ml of 5-FU for 24 h. (E) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/5-FU cells. (F) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 mg/ml). (G) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 μg/ml). (H) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). (I) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). The protein expression of MDR1 and <t>MRP1</t> in SGC-7901/CDDP (J) and SGC-7901/5-FU (L) cells detected by Western blot, FEZF1-AS1 siRNA decreased the protein levels of MDR1 and MRP1 in both cells. The knockdown efficiency of FEZF1-AS1 in SGC-7901/CDDP (K) and SGC-7901/5-FU (M) cells detected by qRT-PCR. Each experiment was performed in triplicate. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Cyp11a1 Pab40042 Bioswamp, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
cyp11a1 pab40042 bioswamp - by Bioz Stars, 2026-04
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Image Search Results


FEZF1-AS1 was upregulated in chemo-resistant GC tissues and knockdown of FEZF1-AS1 improved chemo-sensitivity of GC cells. (A) qRT-PCR on the level of FEZF1-AS1 in 27 pairs of specimens from GC patients with poor response to chemotherapy were 3.5-fold increase compared with patients with good response. (B) CCK8 assay on the viability of SGC-7901 and SGC-7901/CDDP cells treated with 10–80 μg/ml of CDDP for 24 h. (C) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/CDDP cells. (D) CCK8 assay on the viability of SGC-7901 and SGC-7901/5-FU cells treated with 5–25 μg/ml of 5-FU for 24 h. (E) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/5-FU cells. (F) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 mg/ml). (G) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 μg/ml). (H) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). (I) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). The protein expression of MDR1 and MRP1 in SGC-7901/CDDP (J) and SGC-7901/5-FU (L) cells detected by Western blot, FEZF1-AS1 siRNA decreased the protein levels of MDR1 and MRP1 in both cells. The knockdown efficiency of FEZF1-AS1 in SGC-7901/CDDP (K) and SGC-7901/5-FU (M) cells detected by qRT-PCR. Each experiment was performed in triplicate. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: LncRNA FEZF1-AS1 Promotes Multi-Drug Resistance of Gastric Cancer Cells via Upregulating ATG5

doi: 10.3389/fcell.2021.749129

Figure Lengend Snippet: FEZF1-AS1 was upregulated in chemo-resistant GC tissues and knockdown of FEZF1-AS1 improved chemo-sensitivity of GC cells. (A) qRT-PCR on the level of FEZF1-AS1 in 27 pairs of specimens from GC patients with poor response to chemotherapy were 3.5-fold increase compared with patients with good response. (B) CCK8 assay on the viability of SGC-7901 and SGC-7901/CDDP cells treated with 10–80 μg/ml of CDDP for 24 h. (C) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/CDDP cells. (D) CCK8 assay on the viability of SGC-7901 and SGC-7901/5-FU cells treated with 5–25 μg/ml of 5-FU for 24 h. (E) qRT-PCR on the level of FEZF1-AS1 in SGC-7901 and SGC-7901/5-FU cells. (F) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 mg/ml). (G) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/CDDP cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with CDDP (40 μg/ml). (H) Representative images and quantitation of flow cytometric analysis of apoptotic SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). (I) Western blot detected apoptotic proteins, BCL2, BAX, and Cleaved caspase3 in SGC-7901/5-FU cells with or without the treatment of FEZF1-AS1 siRNA after 24 h incubation with 5-FU (20 μg/ml). The protein expression of MDR1 and MRP1 in SGC-7901/CDDP (J) and SGC-7901/5-FU (L) cells detected by Western blot, FEZF1-AS1 siRNA decreased the protein levels of MDR1 and MRP1 in both cells. The knockdown efficiency of FEZF1-AS1 in SGC-7901/CDDP (K) and SGC-7901/5-FU (M) cells detected by qRT-PCR. Each experiment was performed in triplicate. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature and incubated overnight at 4°C with the first antibodies against drug resistance-associated proteins MDR1 (1:2000, PAB30805, Bioswamp), MRP1 (1:1000, PAB33537, Bioswamp), ATG5 (1:1000, 10181-2-AP, Proteintech), β-actin (1:1000, 66009-1-Ig, Proteintech), Ubiquitin (1:1000, 3936, CST), LC3B (1:1000, 3836, CST), BCL2 (1:1000, 12789-1-AP, Proteintech), BAX (1:1000, 50599-2-Ig, Proteintech) and Cleaved caspase3 (1:1000, 9661, CST).

Techniques: Knockdown, Quantitative RT-PCR, CCK-8 Assay, Quantitation Assay, Incubation, Western Blot, Expressing