bcan (Proteintech)
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93
Structured Review
Proteintech
bcan
Bcan, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bcan/product/Proteintech
Average 93 stars, based on 4 article reviews
Bcan, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bcan/product/Proteintech
Average 93 stars, based on 4 article reviews
bcan - by Bioz Stars,
2026-03
93/100 stars
Images
1) Product Images from "Piccolo Regulates Secretion of the Extracellular Matrix Components Brevican and Tenascin R from Astrocytes to Drive Synapse Formation: Implications for Pontocerebellar Hypoplasia Type 3 (PCH3)"
Article Title: Piccolo Regulates Secretion of the Extracellular Matrix Components Brevican and Tenascin R from Astrocytes to Drive Synapse Formation: Implications for Pontocerebellar Hypoplasia Type 3 (PCH3)
Journal: bioRxiv
doi: 10.1101/2025.07.03.662734
Figure Legend Snippet: A, Immunohistological staining for BCAN was performed on cerebellar brain sections from Pclo wt/wt and Pclo gt/gt rats. In both genotypes, BCAN is localized in GFAP-positive astrocytes, from which it is secreted. In Pclo wt/wt slices, BCAN is also found in the surrounding tissue, indicating effective secretion. In contrast, in Pclo gt/gt sections, significantly less BCAN was detected outside astrocytes, suggesting impaired secretion of BCAN from astrocytes. B , Quantification of a. BCAN intensity is slightly greater inside astrocytes in Pclo gt/gt cerebellar sections than in Pclo wt/wt cerebellar slices; however, this difference is not significant ( Pclo wt/wt : mean± SEM= 1± 0.05, n=40 astrocytes, 3 independent animals; Pclo gt/gt : mean± SEM= 1.151± 0.06, 2 independent animals; p=0.082, t test). C, Quantification of a. BCAN intensity is significantly lower outside astrocytes in Pclo gt/gt slices than in Pclo wt/wt slices ( Pclo wt/wt : mean± SEM= 1± 0.04, 3 independent animals; Pclo gt/gt : mean± SEM= 0,831± 0.06, 2 independent animals; p=0.018, t test). Scale bar, 10 μm.
Techniques Used: Staining
Figure Legend Snippet: A, Immunocytochemical staining for BCAN on primary Pclo wt/wt and Pclo gt/gt cortical astrocytes. In Pclo gt/gt astrocytes, BCAN intensity accumulates around the nucleus and extends along a line from the center toward the periphery. In Pclo wt/wt astrocytes, BCAN intensity was distributed more evenly. B , Immunocytochemical staining for TNR on Pclo wt/wt and Pclo gt/gt cortical astrocytes. In Pclo gt/gt astrocytes, TNR intensity accumulates around the nucleus and extends along a line from the cell center toward the cell periphery. In contrast, in Pclo wt/wt astrocytes, the TNR intensity was distributed more evenly. C , Quantification of A. A line was drawn from the nucleus to the periphery and divided into 10 bins. BCAN intensity was subsequently measured within each bin and graphically represented. BCAN intensity is greater close to the nucleus in Pclo gt/gt primary cortical astrocytes than in Pclo wt/wt primary cortical astrocytes. D , Quantification of B. A line was drawn from the nucleus to the periphery and divided into 10 bins, subsequently TNR intensity was measured within each bin. TNR intensity is greater close to the nucleus in Pclo gt/gt cortical astrocytes than in Pclo wt/wt . E , Western blot (WB) analysis of the supernatants of Pclo wt/wt and Pclo gt/gt cortical astrocyte cultures. Significantly less BCAN was detected in Pclo gt/gt cortical supernatants ( Pclo wt/wt : mean= 1; Pclo gt/gt : mean± SEM= 0.81± 0.045, n=3 independent experiments; p=0.0024, t test). F , Western blot (WB) analysis of the supernatants of Pclo wt/wt and Pclo gt/gt cortical astrocyte cultures. Significantly more TNR was detected in supernatant from Pclo gt/gt cortical astrocytes ( Pclo wt/wt : mean=1, n=3 independent experiments; Pclo gt/gt : mean± SEM= 1.653± 0.283, n=3 independent experiments; p=0.082, t test). Scale bar, 10 μm.
Techniques Used: Staining, Western Blot
Figure Legend Snippet: A, Immunocytochemical staining BCAN, GM130, GFAP, and DAPI in Pclo wt/wt and Pclo gt/gt cerebellar astrocytes. BCAN fluorescence intensity is diffusely distributed in Pclo wt/wt astrocytes, whereas it accumulates along a defined path in Pclo gt/gt astrocytes . B, Immunocytochemical staining of tenascin-R (TNR), GM130, GFAP, and DAPI in Pclo wt/wt and Pclo gt/gt cerebellar astrocytes. TNR fluorescence intensity is concentrated near the nucleus and extends toward the cell periphery in Pclo gt/gt astrocytes, in contrast to a more uniform distribution in Pclo wt/wt astrocytes. C, Schematic illustration of the cargo distribution analysis method. A line from the nucleus to the cell periphery was segmented into 10 equal bins (0–9), and the fluorescence intensity of the cargo was measured in each bin and normalized to the mean intensity per bin in Pclo wt/wt astrocytes. D, Quantification of a. Normalized BCAN intensity decreases progressively from the nucleus to the periphery in Pclo wt/wt astrocytes but slightly increases in Pclo gt/gt astrocytes. E, Quantification of B. Normalized TNR intensity is greater near the nucleus in Pclo gt/gt astrocytes than in Pclo wt/wt cells. F, Western blot (WB) analysis of BCAN levels in the supernatants of Pclo wt/wt and Pclo gt/gt cerebellar astrocytes. BCAN levels are reduced in Pclo gt/gt astrocytes ( Pclo wt/wt : mean= 1; Pclo gt/gt : mean± SEM= 0.641± 0.141, n=3 independent experiments; p=0.064, t test). G, Western blot (WB) analysis of TNR levels in the supernatants of Pclo wt/wt and Pclo gt/gt cerebellar astrocytes. TNR levels are increased in Pclo gt/gt astrocytes ( Pclo wt/wt : mean= 1; Pclo gt/gt : mean± SEM= 1.246± 0.179, n=3 independent experiments; p=0.242, t test). Scale bar, 10 μm.
Techniques Used: Staining, Fluorescence, Western Blot
