Journal: Environmental Health

Article Title: Serum dioxin and DNA methylation in the sperm of operation ranch hand veterans exposed to Agent Orange

doi: 10.1186/s12940-019-0533-z

Figure Lengend Snippet: Top 50 EWAS loci ranked by the change in beta value comparing highest exposed with control

Article Snippet: SPAM1 , cg04471202 , 7 , 906 , 0.23 , 6.00E-04 , 0.31 , 0.17.

Techniques:

Journal: Scientific reports

Article Title: Microsomal membrane proteome of low grade diffuse astrocytomas: Differentially expressed proteins and candidate surveillance biomarkers.

doi: 10.1038/srep26882

Figure Lengend Snippet: Figure 3. Verification of differential expression of the representative proteins observed in LC-MS/MS analysis by immunohistochemistry on tissue sections. (A) shows MS/MS spectra of peptides with their reporter ion intensities for representative differentially expressed proteins - BCAN, EGFR, ENPP6 and HNRNP K. (B) immunohistochemistry (IHC) images acquired for the above proteins. IHC protocol is described under Methods and the staining and scoring details for each protein are shown in Supplementary Table S3. For BCAN, normal brain tissue shows low staining with pyramidal cells negative (a), Grade II tumor cells show strong cytoplasmic positivity (b). For EGFR, normal brain tissue shows negative staining (c) and Grade II tumor cells show medium intensity cytoplasmic staining (d). ENPP6 shows medium intensity staining of neurophils in normal brain with no staining of normal glial and neuronal cells (e), while Grade II tumor cells show low to medium intensity staining for ENPP6 in neurophil as well as in tumor cells (f). For HNRNP K, normal brain tissue scored negative (g) whereas Grade II tumor cells showed strong positivity (h).

Article Snippet: Sections were then incubated for 1 h at RT with EGFR (dilution 1:100; Cat No. HPA018530), BCAN (dilution-1:200; Cat No. HPA007865), ENPP6 (dilution-1:10; Cat No. HPA042740) and HNRNP K (dilution-1:250; Cat No. HPA007644) primary antibodies (Atlas Antibodies, Sigma) followed by peroxidase-labelled polymer conjugate to anti-rabbit or anti-mouse immunoglobulins compatible with the primary antibody, for 1 h and developed with DAB system (DAKO, Denmark).

Techniques: Quantitative Proteomics, Liquid Chromatography with Mass Spectroscopy, Immunohistochemistry, Tandem Mass Spectroscopy, Staining, Negative Staining

Journal: Oncology Letters

Article Title: Extracellular matrix differences in glioblastoma patients with different prognoses

doi: 10.3892/ol.2018.9649

Figure Lengend Snippet: List of the analyzed ECM components.

Article Snippet: BCAN , BCAN-Hs00222607_m1.

Techniques:

Journal: Oncology Letters

Article Title: Extracellular matrix differences in glioblastoma patients with different prognoses

doi: 10.3892/ol.2018.9649

Figure Lengend Snippet: Invasion spectrum (the mRNA expression pattern of invasion-associated extracellular matrix components) differs in patients with ‘worse’ and ‘better’ prognoses. mRNA expression measurements were performed twice for each gene to confirm the data. A longer bar on the logarithmic scale indicates reduced expression. *P<0.05 vs. group A (Mann-Whitney U test). Group A, OS <24 months; group B, OS >24 months; BCAN, brevican; CD44, cluster of differentiation 44; CSPG5, chondroitin sulfate proteoglycan 5; EGFR, epidermal growth factor receptor; FLT4, Fms-related tyrosine kinase 4; HMMR, hyaluronan-mediated motility receptor; IDH1, isocitrate dehydrogenase 1; ITGAV, integrin-αV; MDM2, murine double minute 2; MMP-2, matrix metallopeptidase 2; NCAN, neurocan; PDGFA, platelet-derived growth factor α; TNC, tenascin C; VCAN, versican; OS, overall survival.

Article Snippet: BCAN , BCAN-Hs00222607_m1.

Techniques: Expressing, MANN-WHITNEY, Derivative Assay

Journal: Oncology Letters

Article Title: Extracellular matrix differences in glioblastoma patients with different prognoses

doi: 10.3892/ol.2018.9649

Figure Lengend Snippet: Mean immunohistochemistry scores of the ECM components that were determined to be significantly different in the two prognostic groups.

Article Snippet: BCAN , BCAN-Hs00222607_m1.

Techniques: Immunohistochemistry