Journal: Journal of Neurophysiology

Article Title: Differential expression of genes in the RhoA/ROCK pathway in the hippocampus and cortex following intermittent hypoxia and high-intensity interval training

doi: 10.1152/jn.00422.2023

Figure Lengend Snippet: TaqMan gene expression assay information (RhoA/ROCK pathway)

Article Snippet: BCAN , Target , Bcan , Rn00563814_m1 , 71.

Techniques: Gene Expression, TaqMan Assay, Amplification, Membrane

Journal: Environmental Health

Article Title: Serum dioxin and DNA methylation in the sperm of operation ranch hand veterans exposed to Agent Orange

doi: 10.1186/s12940-019-0533-z

Figure Lengend Snippet: Top 50 EWAS loci ranked by the change in beta value comparing highest exposed with control

Article Snippet: SPAM1 , cg04471202 , 7 , 906 , 0.23 , 6.00E-04 , 0.31 , 0.17.

Techniques:

Journal: Scientific reports

Article Title: Microsomal membrane proteome of low grade diffuse astrocytomas: Differentially expressed proteins and candidate surveillance biomarkers.

doi: 10.1038/srep26882

Figure Lengend Snippet: Figure 3. Verification of differential expression of the representative proteins observed in LC-MS/MS analysis by immunohistochemistry on tissue sections. (A) shows MS/MS spectra of peptides with their reporter ion intensities for representative differentially expressed proteins - BCAN, EGFR, ENPP6 and HNRNP K. (B) immunohistochemistry (IHC) images acquired for the above proteins. IHC protocol is described under Methods and the staining and scoring details for each protein are shown in Supplementary Table S3. For BCAN, normal brain tissue shows low staining with pyramidal cells negative (a), Grade II tumor cells show strong cytoplasmic positivity (b). For EGFR, normal brain tissue shows negative staining (c) and Grade II tumor cells show medium intensity cytoplasmic staining (d). ENPP6 shows medium intensity staining of neurophils in normal brain with no staining of normal glial and neuronal cells (e), while Grade II tumor cells show low to medium intensity staining for ENPP6 in neurophil as well as in tumor cells (f). For HNRNP K, normal brain tissue scored negative (g) whereas Grade II tumor cells showed strong positivity (h).

Article Snippet: Sections were then incubated for 1 h at RT with EGFR (dilution 1:100; Cat No. HPA018530), BCAN (dilution-1:200; Cat No. HPA007865), ENPP6 (dilution-1:10; Cat No. HPA042740) and HNRNP K (dilution-1:250; Cat No. HPA007644) primary antibodies (Atlas Antibodies, Sigma) followed by peroxidase-labelled polymer conjugate to anti-rabbit or anti-mouse immunoglobulins compatible with the primary antibody, for 1 h and developed with DAB system (DAKO, Denmark).

Techniques: Quantitative Proteomics, Liquid Chromatography with Mass Spectroscopy, Immunohistochemistry, Tandem Mass Spectroscopy, Staining, Negative Staining