anti atl2  (Proteintech)

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: Distributions of offspring from intercrosses between ATL2 gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Staining, Immunohistochemical staining, Control

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A-B: Western blot analysis of the expression of three ATLs in different mouse tissues (A) and mouse brains from different embryonic stages (B). C: Western blot analysis of the expression of three ATLs in control and ATL2 fl/fl Nes-Cre mice. D-E: Western blot analysis of ATL1 and ATL2 expression in mouse (D) and human (E) cerebrum and cerebellum. F-G: Western blot analysis of different ATLs in neuroglial cells from mouse (F) and human (G) brains. H-I: Western blot analysis of three ATLs in mouse cerebrum (H) and cerebellum (I) at different postnatal times. Data are represented as mean ± SD.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Western Blot, Expressing, Control

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: Schematic of cerebellum structure. ml, molecular layer; pcl, Purkinje cell layer; gcl, granule cell layer; wm, white matter; BG, Bergmann glia; PC, Purkinje cell; AC, astrocyte; GC, granule cell. B-D: Brain image and cerebellum H&E staining of ATL2 fl/fl Pcp2-Cre (B), ATL2 fl/fl Atoh1-Cre (C), ATL2 fl/fl Gfap-Cre (D), and their controls (scale bars, 0.5 cm in brain image, 0.5 mm in H&E staining). E-F: Footprint test (upper panel) and rotarod test (lower panel) of Purkinje neuron (E) and granule neuron (F) ATL1/2 double mutant mice. G-H: H&E staining of sagittal brain sections of granule neuron (G) and Purkinje neuron (H) ATL1/2 double knockout mice (scale bars, 0.5 mm). I-L: Brain images (left panel) and H&E staining of sagittal cerebellum sections (right panel) of ATL2 fl/fl Gfap-Cre mice overexpressing ATL1 (I) or ATL2 (K) (scale bar, 0.5 cm in brain image, 0.5 mm in H&E staining). Footprint and rotarod test of ATL2 fl/fl Gfap-Cre mice overexpressing ATL1 (J) or ATL2 (L). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001, ns, not significant.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Staining, Mutagenesis, Double Knockout

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: GFAP IF staining of cerebellum on postnatal day 7 (P7) in control and ATL2 fl/fl Gfap-Cre mice (scale bar, 50 μm). B: Time-lapse imaging of granule neuron migration in the cerebellum cortex of P7 mice. C: FIB-SEM (upper panel) and 3D reconstruction (lower panel) of Bergmann glia from control and ATL2 fl/fl Gfap-Cre mice (scale bar, 2 μm). D: Volume of endoplasmic reticulum fragment in control and ATL2 fl/fl Gfap-Cre Bergmann glia. E-G: Ratio of total volume of endoplasmic reticulum (E), mitochondria (F) and Golgi (G) to cell volume in control and ATL2-deficient Bergmann glia. H: Lipidomics analysis of the cerebellum of control and ATL2 fl/fl Nes-Cre mice. I: Relative cholesterol in the cerebellum of control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Nes-Cre mice overexpressing ATL1 or ATL2. J: RNA-seq analysis of lipid synthesis-related genes in WT and ATL2/3 DKO COS7 cells. K-M: mRNA level of FDFT1 (K), CHKA, PCYT1B (L), HMGCS1, SCD1 , and SCD2 (M) in primary neuron glia from control and ATL2 fl/fl Nes-Cre mice. Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Staining, Control, Imaging, Migration, RNA Sequencing

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A : Averaged presynaptic ICa (top) and Cm (bottom) induced by depol 20ms (arrow) in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. B-E : Statistics for presynaptic ICa (B), ΔCm (C), Rate endo (D), and ΔCm 15s (E) induced by depol 20ms from control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice (control, n = 8; ATL2 fl/fl Nes-Cre, n = 10; ATL2 fl/fl Gfap-Cre, n = 7; ATL2 fl/fl Atoh1-Cre, n = 7). F : Averaged presynaptic ICa (top) and Cm (bottom) induced by depol 20msx10 (arrow) in the control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. G-J : Statistics for presynaptic QICa (G), ΔCm (H), Rate endo (I), and ΔCm 30s (J) induced by depol 20msx10 from control (black), ATL2 fl/fl Nes-Cre, ATL2 fl/fl Gfap-Cre, and ATL2 fl/fl Atoh1-Cre (green) mice (control, n = 8; ATL2 fl/fl Nes-Cre, n = 10; ATL2 fl/fl Gfap-Cre, n = 7; ATL2 fl/fl Atoh1-Cre, n = 7). K : Sampled ICa (top) and Cm (bottom) induced by 1 (black), 2 (green), 5 (blue), 10 (purple), 20 (yellow), 30 (orange), and 50 ms (red) depolarization pluses from –80 to +10 mV in the control and ATL2 fl/fl Nes-Cre mice. L : The relationship between ΔCm and the duration of depolarization pulses in the control (n = 8 for each data point; black), ATL2 fl/fl Nes-Cre (n = 8 for each data point; red), ATL2 fl/fl Gfap-Cre (n = 8 for each data point; blue), and ATL2 fl/fl Atoh1-Cre (n = 6 for each data point; green) mice. M : Statistics for the release probability measured by the percentage of RRP release induced by a 1-ms depolarization pulse from –80 to +10 mV in the control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice using one-way ANOVA with Dunnett’s post hoc test. ns, not significant. N : Cm induced by a 20-ms depolarization applied at various intervals after the conditional stimulus (depol 20ms ) in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. Left and right panels show the same data at different scales. Data are represented as mean ± SEM. The statistical significance of mean values was analyzed using a one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Control

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: EM image of red presynaptic calyx and green postsynaptic MNTB principal neuron (left). High-resolution EM images of the active zone (red box) within the calyx of Held (right) (scale bar, 1 μm). B-D : Synapse vesicle density of the calyx of Held from ATL2 fl/fl Nes-Cre (B), ATL2 fl/fl Gfap-Cre (C), and ATL2 fl/fl Atoh1-Cre (D) mice and their controls. The red boxes indicate presynaptic active zones (scale bar, 1 μm). E : Representative traces of mEPSC recorded in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. F : Statistics for the mEPSC amplitude, frequency, decay time, and rise time for all groups (control, n = 6; ATL2 fl/fl Nes-Cre, n = 8; ATL2 fl/fl Gfap-Cre, n = 6; ATL2 fl/fl Atoh1-Cre, n = 8). G : Representative EPSC traces recorded in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. H : Statistics for the EPSC amplitude and paired-pulse ratio (PPR) for all groups (control, n = 6; ATL2 fl/fl Nes-Cre, n = 8; ATL2 fl/fl Gfap-Cre, n = 6; ATL2 fl/fl Atoh1-Cre, n = 8). Data in B-D are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, ** p < 0.01, * p < 0.05, ns, not significant. Data in F and G are represented as mean ± SEM. The statistical significance of mean values was analyzed using one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, * p < 0.05, ns, not significant.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Control

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A : Representative ABR traces recorded using tone stimuli in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. Roman numerals identify wave I in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. B : Statistics for the amplitude and latency of wave I at 16 kHz and 90 dB in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. C : ABR thresholds at frequencies of 4, 8, 12, 24, and 32 kHz were recorded using tone stimuli in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. Data are represented as mean ± SEM. The statistical significance of mean values was analyzed using one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, ** p < 0.05, * p < 0.05.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Control

Journal: eLife

Article Title: Zika virus remodels and hijacks IGF2BP2 ribonucleoprotein complex to promote viral replication organelle biogenesis

doi: 10.7554/eLife.94347

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-LARP1 (A302-087A) and anti-ATL2 (A303-332A) come from Thermo Fisher Scientific.

Techniques: Plasmid Preparation, Amplification, Virus, Cloning, Derivative Assay, Transfection, Construct, Control, shRNA, Immunofluorescence, Staining, Recombinant, In Vitro, Sequencing, SYBR Green Assay, ISH Cell Assay, Software

Journal: eLife

Article Title: Zika virus remodels and hijacks IGF2BP2 ribonucleoprotein complex to promote viral replication organelle biogenesis

doi: 10.7554/eLife.94347

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-ATL2 (Rabbit polyclonal) , Thermo Fisher Scientific , Cat#: A303-332A RRID: AB_10971492 , WB (1:1000).

Techniques: Plasmid Preparation, Amplification, Virus, Cloning, Derivative Assay, Transfection, Construct, Control, shRNA, Immunofluorescence, Staining, Recombinant, In Vitro, Sequencing, SYBR Green Assay, ISH Cell Assay, Software

Journal: Genes

Article Title: High Atlastin 2-2 (ATL2-2) Expression Associates with Worse Prognosis in Estrogen-Receptor-Positive Breast Cancer.

doi: 10.3390/genes14081559

Figure Lengend Snippet: Figure 1. ATL2-2 is more highly expressed in breast tumors than normal breast tissue. (A) ATL2-2 transcript levels were compared between breast tumors and normal (non-neoplastic) breast tissue samples from Cohort 2 using a Student’s t-test (263 tumors vs. 36 normal tissue), p = 6.0 × 10−6, and (B) in 36 tumors and their corresponding adjacent normal tissue using a paired t-test, p = 0.05. Tumors are depicted in yellow and normal tissue in blue. (C) ATL2 protein expression was detected by ATL2 antibody in tumor cells (granular brown cytoplasmic stain) and adjacent normal cells (faint brown). A representative figure from immunohistochemical analysis of 13 tumor–normal pairs. The magnification was 40x. ATL2 protein expression was mostly observed in the cytoplasm in granules and as a diffuse stain.

Article Snippet: Tissue microarrays (TMA) from 13 invasive breast tumors and adjacent normal (nonneoplastic) breast tissue were stained with anti-ATL2 antibody (HPA029108, Atlas Antibodies, 0.1 mg/mL, Stockholm, Sweden).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: Genes

Article Title: High Atlastin 2-2 (ATL2-2) Expression Associates with Worse Prognosis in Estrogen-Receptor-Positive Breast Cancer.

doi: 10.3390/genes14081559

Figure Lengend Snippet: Figure 2. ATL2-2 mRNA expression levels were higher in estrogen-receptor-negative tumors as compared to estrogen-receptor-positive tumors. A correlation analysis between ATL2-2 mRNA levels and estrogen receptor status was performed in Cohort 1, Cohort 2, TCGA, and METABRIC. The number of estrogen-receptor-negative and -positive tumors are shown below each boxplot. The difference in ATL2-2 expression between the estrogen-receptor-negative and -positive categories was calculated with a t-test with unequal variance. Cohort 1: p = 0.089, Cohort 2: p = 4 × 10−5, TCGA: p = 0.083, METABRIC: p = 2.2 × 10−16.

Article Snippet: Tissue microarrays (TMA) from 13 invasive breast tumors and adjacent normal (nonneoplastic) breast tissue were stained with anti-ATL2 antibody (HPA029108, Atlas Antibodies, 0.1 mg/mL, Stockholm, Sweden).

Techniques: Expressing

Journal: Genes

Article Title: High Atlastin 2-2 (ATL2-2) Expression Associates with Worse Prognosis in Estrogen-Receptor-Positive Breast Cancer.

doi: 10.3390/genes14081559

Figure Lengend Snippet: Figure 3. High ATL2-2 mRNA levels associated with shorter breast-cancer-specific survival in patients with estrogen-receptor-positive luminal tumors. Breast-cancer-specific survival (BCSS) was analyzed in the METABRIC cohort in patients whose tumors expressed the estrogen receptor and were classified as luminal according to molecular subtyping. The ATL2-2 mRNA values in the patients’ tumors were divided based on the max stat function that finds the best division based on outcome. In the low-expressing group, there were 594 (black line), and there were 503 in the group expressing high ATL2-2 (red line). The log rank p-value was 2 × 10−4. The number of patients at risk at the indicated time point is shown in a table below the Kaplan–Meier graph. The HR was 1.535 (CI 1.228–1.918) prior to adjusting for confounding variables. Table 1 shows the hazard ratios (HRs) and confidence interval (CI) from the Cox regression analysis prior to and after adjusting for confounding variables.

Article Snippet: Tissue microarrays (TMA) from 13 invasive breast tumors and adjacent normal (nonneoplastic) breast tissue were stained with anti-ATL2 antibody (HPA029108, Atlas Antibodies, 0.1 mg/mL, Stockholm, Sweden).

Techniques: Expressing

Journal: Genes

Article Title: High Atlastin 2-2 (ATL2-2) Expression Associates with Worse Prognosis in Estrogen-Receptor-Positive Breast Cancer.

doi: 10.3390/genes14081559

Figure Lengend Snippet: Figure 4. Proliferative pathways were enriched in tumors that express high ATL2-2 mRNA levels. Gene set enrichment analysis was used to analyze which Hallmark pathways were upregulated in tumors with high ATL2-2 mRNA levels. The results show the top three pathways identified in the whole METABRIC cohort, which were also the only three pathways observed in estrogen-receptor- positive luminal B tumors in both METABRIC and TCGA cohorts.

Article Snippet: Tissue microarrays (TMA) from 13 invasive breast tumors and adjacent normal (nonneoplastic) breast tissue were stained with anti-ATL2 antibody (HPA029108, Atlas Antibodies, 0.1 mg/mL, Stockholm, Sweden).

Techniques: