atl2 Search Results


atl2  (Sino Biological)
90
Sino Biological atl2
Atl2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atl2/sino+biological___atl2?v=Sino+Biological
Average 90 stars, based on 1 article reviews
atl2 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
atl2  (Proteintech)
93
Proteintech atl2
Atl2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atl2/pmc09169640__pnas__2104309119__sapp-62-10-11?v=Proteintech
Average 93 stars, based on 1 article reviews
atl2 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
conditional gene overexpression  (Cyagen Biosciences)
93
Cyagen Biosciences conditional gene overexpression
A: Distributions of offspring from intercrosses between <t>ATL2</t> gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.
Conditional Gene Overexpression, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atl2/bio_rxiv__2025__04__12__648519-247-2-15?v=Cyagen+Biosciences
Average 93 stars, based on 1 article reviews
conditional gene overexpression - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
breast tumors  (Atlas Antibodies)
91
Atlas Antibodies breast tumors
A: Distributions of offspring from intercrosses between <t>ATL2</t> gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.
Breast Tumors, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atl2/pm37628611-80-6-20?v=Atlas+Antibodies
Average 91 stars, based on 1 article reviews
breast tumors - by Bioz Stars, 2026-06
91/100 stars
  Buy from Supplier
pcdna3 1 egfp  (Addgene inc)
90
Addgene inc pcdna3 1 egfp
A: Distributions of offspring from intercrosses between <t>ATL2</t> gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.
Pcdna3 1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atl2/pmc09226543-54-14-16?v=Addgene+inc
Average 90 stars, based on 1 article reviews
pcdna3 1 egfp - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
sirna targeting atl2  (Ribobio co)
90
Ribobio co sirna targeting atl2
A: Distributions of offspring from intercrosses between <t>ATL2</t> gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.
Sirna Targeting Atl2, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atl2/pmc06628656__pnas__1908409116__sapp-30-5-22?v=Ribobio+co
Average 90 stars, based on 1 article reviews
sirna targeting atl2 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
mirna 3´utr target expression clone for human atl2  (Genecopoeia)
N/A
GeneCopoeia offers genome-wide human, mouse and rat microRNA (miRNA) 3′ UTR target clones in mammalian expression vectors. miRNA 3′ UTR target clones can be used for miRNA target identification and functional validation of predicted targets,
   Buy from Supplier
ad-h-atl2-shrna  (Vector Biolabs)
N/A
   Buy from Supplier
atl2 antibody (hrp)  (biorbyt)
N/A
Rabbit polyclonal antibody against ATL2 conjugated to HRP. Isotype Note: IgG Host Note: Rabbit Conjugation Note: HRP Reactivity Note: Human Application Note: ELISA
   Buy from Supplier
atl2 nm 019717 mouse tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Atl2 Myc DDK tagged Mouse atlastin GTPase 2 Atl2 transcript variant 1
   Buy from Supplier
arl6ip2 atl2 nm 022374 human 3 utr clone  (OriGene)
N/A
3 UTR clone of atlastin GTPase 2 ATL2 transcript variant 1 for miRNA target validation
   Buy from Supplier
atl2 mouse shrna lentiviral particle  (OriGene)
N/A
Atl2 Mouse shRNA lentiviral particles 4 unique 29mer target specific shRNA 1 scramble control 0 5 ml each 10 7 TU ml
   Buy from Supplier

Image Search Results


A: Distributions of offspring from intercrosses between ATL2 gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: Distributions of offspring from intercrosses between ATL2 gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Staining, Immunohistochemical staining, Control

A-B: Western blot analysis of the expression of three ATLs in different mouse tissues (A) and mouse brains from different embryonic stages (B). C: Western blot analysis of the expression of three ATLs in control and ATL2 fl/fl Nes-Cre mice. D-E: Western blot analysis of ATL1 and ATL2 expression in mouse (D) and human (E) cerebrum and cerebellum. F-G: Western blot analysis of different ATLs in neuroglial cells from mouse (F) and human (G) brains. H-I: Western blot analysis of three ATLs in mouse cerebrum (H) and cerebellum (I) at different postnatal times. Data are represented as mean ± SD.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A-B: Western blot analysis of the expression of three ATLs in different mouse tissues (A) and mouse brains from different embryonic stages (B). C: Western blot analysis of the expression of three ATLs in control and ATL2 fl/fl Nes-Cre mice. D-E: Western blot analysis of ATL1 and ATL2 expression in mouse (D) and human (E) cerebrum and cerebellum. F-G: Western blot analysis of different ATLs in neuroglial cells from mouse (F) and human (G) brains. H-I: Western blot analysis of three ATLs in mouse cerebrum (H) and cerebellum (I) at different postnatal times. Data are represented as mean ± SD.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Western Blot, Expressing, Control

A: Schematic of cerebellum structure. ml, molecular layer; pcl, Purkinje cell layer; gcl, granule cell layer; wm, white matter; BG, Bergmann glia; PC, Purkinje cell; AC, astrocyte; GC, granule cell. B-D: Brain image and cerebellum H&E staining of ATL2 fl/fl Pcp2-Cre (B), ATL2 fl/fl Atoh1-Cre (C), ATL2 fl/fl Gfap-Cre (D), and their controls (scale bars, 0.5 cm in brain image, 0.5 mm in H&E staining). E-F: Footprint test (upper panel) and rotarod test (lower panel) of Purkinje neuron (E) and granule neuron (F) ATL1/2 double mutant mice. G-H: H&E staining of sagittal brain sections of granule neuron (G) and Purkinje neuron (H) ATL1/2 double knockout mice (scale bars, 0.5 mm). I-L: Brain images (left panel) and H&E staining of sagittal cerebellum sections (right panel) of ATL2 fl/fl Gfap-Cre mice overexpressing ATL1 (I) or ATL2 (K) (scale bar, 0.5 cm in brain image, 0.5 mm in H&E staining). Footprint and rotarod test of ATL2 fl/fl Gfap-Cre mice overexpressing ATL1 (J) or ATL2 (L). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001, ns, not significant.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: Schematic of cerebellum structure. ml, molecular layer; pcl, Purkinje cell layer; gcl, granule cell layer; wm, white matter; BG, Bergmann glia; PC, Purkinje cell; AC, astrocyte; GC, granule cell. B-D: Brain image and cerebellum H&E staining of ATL2 fl/fl Pcp2-Cre (B), ATL2 fl/fl Atoh1-Cre (C), ATL2 fl/fl Gfap-Cre (D), and their controls (scale bars, 0.5 cm in brain image, 0.5 mm in H&E staining). E-F: Footprint test (upper panel) and rotarod test (lower panel) of Purkinje neuron (E) and granule neuron (F) ATL1/2 double mutant mice. G-H: H&E staining of sagittal brain sections of granule neuron (G) and Purkinje neuron (H) ATL1/2 double knockout mice (scale bars, 0.5 mm). I-L: Brain images (left panel) and H&E staining of sagittal cerebellum sections (right panel) of ATL2 fl/fl Gfap-Cre mice overexpressing ATL1 (I) or ATL2 (K) (scale bar, 0.5 cm in brain image, 0.5 mm in H&E staining). Footprint and rotarod test of ATL2 fl/fl Gfap-Cre mice overexpressing ATL1 (J) or ATL2 (L). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001, ns, not significant.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Staining, Mutagenesis, Double Knockout

A: GFAP IF staining of cerebellum on postnatal day 7 (P7) in control and ATL2 fl/fl Gfap-Cre mice (scale bar, 50 μm). B: Time-lapse imaging of granule neuron migration in the cerebellum cortex of P7 mice. C: FIB-SEM (upper panel) and 3D reconstruction (lower panel) of Bergmann glia from control and ATL2 fl/fl Gfap-Cre mice (scale bar, 2 μm). D: Volume of endoplasmic reticulum fragment in control and ATL2 fl/fl Gfap-Cre Bergmann glia. E-G: Ratio of total volume of endoplasmic reticulum (E), mitochondria (F) and Golgi (G) to cell volume in control and ATL2-deficient Bergmann glia. H: Lipidomics analysis of the cerebellum of control and ATL2 fl/fl Nes-Cre mice. I: Relative cholesterol in the cerebellum of control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Nes-Cre mice overexpressing ATL1 or ATL2. J: RNA-seq analysis of lipid synthesis-related genes in WT and ATL2/3 DKO COS7 cells. K-M: mRNA level of FDFT1 (K), CHKA, PCYT1B (L), HMGCS1, SCD1 , and SCD2 (M) in primary neuron glia from control and ATL2 fl/fl Nes-Cre mice. Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: GFAP IF staining of cerebellum on postnatal day 7 (P7) in control and ATL2 fl/fl Gfap-Cre mice (scale bar, 50 μm). B: Time-lapse imaging of granule neuron migration in the cerebellum cortex of P7 mice. C: FIB-SEM (upper panel) and 3D reconstruction (lower panel) of Bergmann glia from control and ATL2 fl/fl Gfap-Cre mice (scale bar, 2 μm). D: Volume of endoplasmic reticulum fragment in control and ATL2 fl/fl Gfap-Cre Bergmann glia. E-G: Ratio of total volume of endoplasmic reticulum (E), mitochondria (F) and Golgi (G) to cell volume in control and ATL2-deficient Bergmann glia. H: Lipidomics analysis of the cerebellum of control and ATL2 fl/fl Nes-Cre mice. I: Relative cholesterol in the cerebellum of control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Nes-Cre mice overexpressing ATL1 or ATL2. J: RNA-seq analysis of lipid synthesis-related genes in WT and ATL2/3 DKO COS7 cells. K-M: mRNA level of FDFT1 (K), CHKA, PCYT1B (L), HMGCS1, SCD1 , and SCD2 (M) in primary neuron glia from control and ATL2 fl/fl Nes-Cre mice. Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Staining, Control, Imaging, Migration, RNA Sequencing

A : Averaged presynaptic ICa (top) and Cm (bottom) induced by depol 20ms (arrow) in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. B-E : Statistics for presynaptic ICa (B), ΔCm (C), Rate endo (D), and ΔCm 15s (E) induced by depol 20ms from control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice (control, n = 8; ATL2 fl/fl Nes-Cre, n = 10; ATL2 fl/fl Gfap-Cre, n = 7; ATL2 fl/fl Atoh1-Cre, n = 7). F : Averaged presynaptic ICa (top) and Cm (bottom) induced by depol 20msx10 (arrow) in the control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. G-J : Statistics for presynaptic QICa (G), ΔCm (H), Rate endo (I), and ΔCm 30s (J) induced by depol 20msx10 from control (black), ATL2 fl/fl Nes-Cre, ATL2 fl/fl Gfap-Cre, and ATL2 fl/fl Atoh1-Cre (green) mice (control, n = 8; ATL2 fl/fl Nes-Cre, n = 10; ATL2 fl/fl Gfap-Cre, n = 7; ATL2 fl/fl Atoh1-Cre, n = 7). K : Sampled ICa (top) and Cm (bottom) induced by 1 (black), 2 (green), 5 (blue), 10 (purple), 20 (yellow), 30 (orange), and 50 ms (red) depolarization pluses from –80 to +10 mV in the control and ATL2 fl/fl Nes-Cre mice. L : The relationship between ΔCm and the duration of depolarization pulses in the control (n = 8 for each data point; black), ATL2 fl/fl Nes-Cre (n = 8 for each data point; red), ATL2 fl/fl Gfap-Cre (n = 8 for each data point; blue), and ATL2 fl/fl Atoh1-Cre (n = 6 for each data point; green) mice. M : Statistics for the release probability measured by the percentage of RRP release induced by a 1-ms depolarization pulse from –80 to +10 mV in the control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice using one-way ANOVA with Dunnett’s post hoc test. ns, not significant. N : Cm induced by a 20-ms depolarization applied at various intervals after the conditional stimulus (depol 20ms ) in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. Left and right panels show the same data at different scales. Data are represented as mean ± SEM. The statistical significance of mean values was analyzed using a one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A : Averaged presynaptic ICa (top) and Cm (bottom) induced by depol 20ms (arrow) in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. B-E : Statistics for presynaptic ICa (B), ΔCm (C), Rate endo (D), and ΔCm 15s (E) induced by depol 20ms from control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice (control, n = 8; ATL2 fl/fl Nes-Cre, n = 10; ATL2 fl/fl Gfap-Cre, n = 7; ATL2 fl/fl Atoh1-Cre, n = 7). F : Averaged presynaptic ICa (top) and Cm (bottom) induced by depol 20msx10 (arrow) in the control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. G-J : Statistics for presynaptic QICa (G), ΔCm (H), Rate endo (I), and ΔCm 30s (J) induced by depol 20msx10 from control (black), ATL2 fl/fl Nes-Cre, ATL2 fl/fl Gfap-Cre, and ATL2 fl/fl Atoh1-Cre (green) mice (control, n = 8; ATL2 fl/fl Nes-Cre, n = 10; ATL2 fl/fl Gfap-Cre, n = 7; ATL2 fl/fl Atoh1-Cre, n = 7). K : Sampled ICa (top) and Cm (bottom) induced by 1 (black), 2 (green), 5 (blue), 10 (purple), 20 (yellow), 30 (orange), and 50 ms (red) depolarization pluses from –80 to +10 mV in the control and ATL2 fl/fl Nes-Cre mice. L : The relationship between ΔCm and the duration of depolarization pulses in the control (n = 8 for each data point; black), ATL2 fl/fl Nes-Cre (n = 8 for each data point; red), ATL2 fl/fl Gfap-Cre (n = 8 for each data point; blue), and ATL2 fl/fl Atoh1-Cre (n = 6 for each data point; green) mice. M : Statistics for the release probability measured by the percentage of RRP release induced by a 1-ms depolarization pulse from –80 to +10 mV in the control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice using one-way ANOVA with Dunnett’s post hoc test. ns, not significant. N : Cm induced by a 20-ms depolarization applied at various intervals after the conditional stimulus (depol 20ms ) in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. Left and right panels show the same data at different scales. Data are represented as mean ± SEM. The statistical significance of mean values was analyzed using a one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Control

A: EM image of red presynaptic calyx and green postsynaptic MNTB principal neuron (left). High-resolution EM images of the active zone (red box) within the calyx of Held (right) (scale bar, 1 μm). B-D : Synapse vesicle density of the calyx of Held from ATL2 fl/fl Nes-Cre (B), ATL2 fl/fl Gfap-Cre (C), and ATL2 fl/fl Atoh1-Cre (D) mice and their controls. The red boxes indicate presynaptic active zones (scale bar, 1 μm). E : Representative traces of mEPSC recorded in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. F : Statistics for the mEPSC amplitude, frequency, decay time, and rise time for all groups (control, n = 6; ATL2 fl/fl Nes-Cre, n = 8; ATL2 fl/fl Gfap-Cre, n = 6; ATL2 fl/fl Atoh1-Cre, n = 8). G : Representative EPSC traces recorded in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. H : Statistics for the EPSC amplitude and paired-pulse ratio (PPR) for all groups (control, n = 6; ATL2 fl/fl Nes-Cre, n = 8; ATL2 fl/fl Gfap-Cre, n = 6; ATL2 fl/fl Atoh1-Cre, n = 8). Data in B-D are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, ** p < 0.01, * p < 0.05, ns, not significant. Data in F and G are represented as mean ± SEM. The statistical significance of mean values was analyzed using one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, * p < 0.05, ns, not significant.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: EM image of red presynaptic calyx and green postsynaptic MNTB principal neuron (left). High-resolution EM images of the active zone (red box) within the calyx of Held (right) (scale bar, 1 μm). B-D : Synapse vesicle density of the calyx of Held from ATL2 fl/fl Nes-Cre (B), ATL2 fl/fl Gfap-Cre (C), and ATL2 fl/fl Atoh1-Cre (D) mice and their controls. The red boxes indicate presynaptic active zones (scale bar, 1 μm). E : Representative traces of mEPSC recorded in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. F : Statistics for the mEPSC amplitude, frequency, decay time, and rise time for all groups (control, n = 6; ATL2 fl/fl Nes-Cre, n = 8; ATL2 fl/fl Gfap-Cre, n = 6; ATL2 fl/fl Atoh1-Cre, n = 8). G : Representative EPSC traces recorded in control (black), ATL2 fl/fl Nes-Cre (red), ATL2 fl/fl Gfap-Cre (blue), and ATL2 fl/fl Atoh1-Cre (green) mice. H : Statistics for the EPSC amplitude and paired-pulse ratio (PPR) for all groups (control, n = 6; ATL2 fl/fl Nes-Cre, n = 8; ATL2 fl/fl Gfap-Cre, n = 6; ATL2 fl/fl Atoh1-Cre, n = 8). Data in B-D are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, ** p < 0.01, * p < 0.05, ns, not significant. Data in F and G are represented as mean ± SEM. The statistical significance of mean values was analyzed using one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, * p < 0.05, ns, not significant.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Control

A : Representative ABR traces recorded using tone stimuli in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. Roman numerals identify wave I in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. B : Statistics for the amplitude and latency of wave I at 16 kHz and 90 dB in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. C : ABR thresholds at frequencies of 4, 8, 12, 24, and 32 kHz were recorded using tone stimuli in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. Data are represented as mean ± SEM. The statistical significance of mean values was analyzed using one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, ** p < 0.05, * p < 0.05.

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A : Representative ABR traces recorded using tone stimuli in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. Roman numerals identify wave I in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. B : Statistics for the amplitude and latency of wave I at 16 kHz and 90 dB in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. C : ABR thresholds at frequencies of 4, 8, 12, 24, and 32 kHz were recorded using tone stimuli in control, ATL2 fl/fl Nes-Cre, and ATL2 fl/fl Gfap-Cre mice. Data are represented as mean ± SEM. The statistical significance of mean values was analyzed using one-way ANOVA with Dunnett’s post hoc test, *** p < 0.001, ** p < 0.05, * p < 0.05.

Article Snippet: Animals with conditional gene overexpression ( lsl-Atl1 , lsl-Atl2 , lsl-Cdh2 ) were constructed by Cyagen using CRISPR-Cas9.

Techniques: Control