Journal: Neural Regeneration Research
Article Title: Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury
doi: 10.4103/NRR.NRR-D-23-01130
Figure Lengend Snippet: Gas6/Axl signal pathway is inactive in reactive and scar-forming astrocyte after SCI. (A) The violin plot analyses were shown the change of the Gas6 and Axl expression in naive, reactive and scar-forming astrocytes clusters after SCI. (B) Immunofluorescence analysis of Ascc1 + (green, FITC) cells and Gas6 (red, Alexa Fluor 594) immunopositivity in the injured spinal cord. Ascc1 + cells and the immunopositivity of Gas6 were significantly decreased after SCI. (C) Immunofluorescence analysis of Nestin + (green, FITC) cells and Gas6 (red, Alexa Fluor 594) immunopositivity in the injured spinal cord. Nestin + cells were increased and the immunopositivity of Gas6 was significantly decreased after SCI. (D) Immunofluorescence analysis of Bcan + (green, FITC) cells and Gas6 (red, Alexa Fluor 594) immunopositivity in the injured spinal cord. Bcan + cells were increased and the immunopositivity of Gas6 was significantly decreased after SCI. (E) Immunofluorescence analysis of Ascc1 + (red, Alexa Fluor 594) cells and Axl (green, FITC) immunopositivity in the injured spinal cord. Ascc1 + cells and immunopositivity of Axl were significantly decreased after SCI. (F) Immunofluorescence analysis of Nestin + (red, Alexa Fluor 594) cells and Axl (green, FITC) immunopositivity in the injured spinal cord. Nestin + cells were increased and the immunopositivity of Axl was significantly decreased after SCI. (G) Immunofluorescence analysis of Bcan + (red, Alexa Fluor 594) cells and Axl (green, FITC) immunopositivity in the injured spinal cord. Bcan + cells were increased and the immunopositivity of Axl was significantly decreased after SCI. Scale bars: 100 μm (upper), 20 μm (lower). The data are presented as the means ± SD ( n = 3). ** P < 0.01, vs . sham group (unpaired t -test). A.U.: Arbitrary unit; Axl: AXL receptor tyrosine kinase; Bcan: brevican; DAPI: 4′,6-diamidino-2-phenylindole; FITC: fluorescein isothiocyanate isomer I; Gas6: growth arrest specific protein 6; SCI: spinal cord injury.
Article Snippet: ASCC1 , 1:200 , Origene , Rockville, MD, USA , TA503721S , AB_11127958.
Techniques: Expressing, Immunofluorescence
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