Journal: Neural Regeneration Research

Article Title: Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

doi: 10.4103/NRR.NRR-D-23-01130

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: ASCC1 , 1:200 , Origene , Rockville, MD, USA , TA503721S , AB_11127958.

Techniques:

Journal: Neural Regeneration Research

Article Title: Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

doi: 10.4103/NRR.NRR-D-23-01130

Figure Lengend Snippet: Gas6/Axl signal pathway is inactive in reactive and scar-forming astrocyte after SCI. (A) The violin plot analyses were shown the change of the Gas6 and Axl expression in naive, reactive and scar-forming astrocytes clusters after SCI. (B) Immunofluorescence analysis of Ascc1 + (green, FITC) cells and Gas6 (red, Alexa Fluor 594) immunopositivity in the injured spinal cord. Ascc1 + cells and the immunopositivity of Gas6 were significantly decreased after SCI. (C) Immunofluorescence analysis of Nestin + (green, FITC) cells and Gas6 (red, Alexa Fluor 594) immunopositivity in the injured spinal cord. Nestin + cells were increased and the immunopositivity of Gas6 was significantly decreased after SCI. (D) Immunofluorescence analysis of Bcan + (green, FITC) cells and Gas6 (red, Alexa Fluor 594) immunopositivity in the injured spinal cord. Bcan + cells were increased and the immunopositivity of Gas6 was significantly decreased after SCI. (E) Immunofluorescence analysis of Ascc1 + (red, Alexa Fluor 594) cells and Axl (green, FITC) immunopositivity in the injured spinal cord. Ascc1 + cells and immunopositivity of Axl were significantly decreased after SCI. (F) Immunofluorescence analysis of Nestin + (red, Alexa Fluor 594) cells and Axl (green, FITC) immunopositivity in the injured spinal cord. Nestin + cells were increased and the immunopositivity of Axl was significantly decreased after SCI. (G) Immunofluorescence analysis of Bcan + (red, Alexa Fluor 594) cells and Axl (green, FITC) immunopositivity in the injured spinal cord. Bcan + cells were increased and the immunopositivity of Axl was significantly decreased after SCI. Scale bars: 100 μm (upper), 20 μm (lower). The data are presented as the means ± SD ( n = 3). ** P < 0.01, vs . sham group (unpaired t -test). A.U.: Arbitrary unit; Axl: AXL receptor tyrosine kinase; Bcan: brevican; DAPI: 4′,6-diamidino-2-phenylindole; FITC: fluorescein isothiocyanate isomer I; Gas6: growth arrest specific protein 6; SCI: spinal cord injury.

Article Snippet: ASCC1 , 1:200 , Origene , Rockville, MD, USA , TA503721S , AB_11127958.

Techniques: Expressing, Immunofluorescence

Journal: Molecular Cell

Article Title: The ASC-1 Complex Disassembles Collided Ribosomes

doi: 10.1016/j.molcel.2020.06.006

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-ASCC1 , Bethyl , Cat. #A303-871A.

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Sequencing, Mutagenesis, Software, Cloning

Journal: The Application of Clinical Genetics

Article Title: RAI1 gene mutations: mechanisms of Smith–Magenis syndrome

doi: 10.2147/TACG.S128455

Figure Lengend Snippet: Schematic representation of chromosome 17, common 17p11.2 deletion, and RAI1 . From left to right, the following are shown: the ideogram of G-band pattern of human chromosome 17; a schematic representation of the Smith–Magenis syndrome region with some representative genes; the RAI1 genomic and protein structure – glutamine-rich domain (Poly-Q), bipartite nuclear localization signals (NLS1 and NLS2), two serine-rich domains (Poly-S1 and Poly-S2), and C-terminal plant homeodomain (PHD); and the RAI1 mutations indexed in the HGMD™ Professional (version December 2015.4) known to date. * Indicates either frameshift or nonsense mutations.

Article Snippet: – A recent study by Thaker et al identified a de novo RAI1 mutation in a child with morbid obesity and a clinical diagnosis of the Rapid-onset Obesity with Hypothalamic dysfunction, Hypoventilation and Autonomic Dysregulation (ROHHAD) syndrome.

Techniques: