Journal: Bioactive Materials
Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
doi: 10.1016/j.bioactmat.2025.10.023
Figure Lengend Snippet: MXMoS 2 DNAgel alleviates inflammation and promotes osteogenesis by modulating macrophage polarization. (A) qRT-PCR analysis of NOS 2 , Il6 , Arg 1 and Il10 levels of RAW264.7 cells after 24h of inflammatory stimulation. (B) ELISA quantification of IL-6 and IL-10 levels of RAW264.7 cells after 24h of inflammatory stimulation. (C – D) Quantitative analysis and representative immunofluorescence staining of iNOS and ARG1. (E) Representative dot plots showing the distribution of iNOS and ARG1 expression in macrophages isolated from POM C57BL/6 mice after DNA hydrogel, MXene/MoS 2 DNA hydrogel, and MXene/MoS 2 DNA hydrogel with NIR irradiation treatment. (F) The stacked bar graphs summarize the proportions of four macrophage subsets (iNOS + ARG1 - , iNOS − ARG1 + , iNOS − ARG1 - , iNOS + ARG1 + ) across groups (n = 3). (G) qRT-PCR analysis of Alp , Runx2 and Sp7 levels of MC3T3-E1 cells after 7 days of osteogenic induction under different treatments. (H) ALP staining of MC3T3-E1 after 7 days. (I) Quantitative analysis of ALP staining. (NC: control group, PC: LPS-treated group) ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.
Article Snippet: Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China).
Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing, Isolation, Irradiation, Control
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