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Image Search Results
Journal: Journal for Immunotherapy of Cancer
Article Title: Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma
doi: 10.1136/jitc-2025-014080
Figure Lengend Snippet: MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) Arg1 expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.
Article Snippet: Primers include murine Gapdh (Mm99999915_g1), B2m (Mm00437762_m1), Arg1 (
Techniques: Activation Assay, Expressing, Flow Cytometry, In Vitro, Reverse Transcription, Derivative Assay, Fluorescence
Journal: iScience
Article Title: Nanosphere pharmacodynamics improves safety of immunostimulatory cytokine therapy
doi: 10.1016/j.isci.2024.108836
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Staining, Cell Culture, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Isolation, Multiplex Assay, Software, Sterility