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Nacalai aitc
Aitc, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aitc/aitc/pm39955887-37-0-18
Average 90 stars, based on 1 article reviews

aitc - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Cytotoxicity of allyl isothiocyanate and its metabolites in hepatocellular carcinoma HepG2 cells.
Article Snippet: Objective: To investigate the mechanisms by which allyl isothiocyanate (AITC) exerts its anticancer effects, the present study investigated the cytotoxic effects of AITC and its metabolites on hepatocellular carcinoma HepG2

Article Title: TRPA1-expressing lamina propria mesenchymal cells regulate colonic motility
Article Snippet: The TRPA1 agonist, AITC, was obtained from Nacalai Tesque.

Article Title: Involvement of nociceptive transient receptor potential channels in repellent action of pulegone.
Article Snippet: Accepted Manuscript Involvement of nociceptive transient receptor potential channels in repellent action of pulegone Azusa Majikina, Kenji Takahashi, Shigeru Saito, Makoto Tominaga, Toshio Ohta PII: S0006-2952(18)30089-3 DOI: https://doi.org/10.1016/j.bcp.2018.02.032 Reference: BCP 13077 To appear in: Biochemical Pharmacology Received Date: 23 January 2018 Accepted Date: 26 February 2018 Please cite this article as: A. Majikina, K. Takahashi, S. Saito, M. Tominaga, T. Ohta, Involvement of nociceptive transient receptor potential channels in repellent action of pulegone, Biochemical Pharmacology (2018), doi: https:// doi.org/10.1016/j.bcp.2018.02.032 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

In Vitro:

Article Title: Negative Regulation of TRPA1 by AMPK in Primary Sensory Neurons as a Potential Mechanism of Painful Diabetic Neuropathy.
Article Snippet: .. The following reagents were from commercial sources: AITC, Nacalai Tesque (Kyoto, Japan); capsaicin, AICAR (used for in vitro experiments), and compound C, Sigma-Aldrich; AICAR (used for behavioral tests), Abcam (Cambridge, MA, USA); metformin, Enzo Life Sciences (Farmingdale, NY, USA); and HC030031, Alomone Labs. ..

Modification:

Article Title: Carboplatin Enhances the Activity of Human Transient Receptor Potential Ankyrin 1 through the Cyclic AMP-Protein Kinase A-A-Kinase Anchoring Protein (AKAP) Pathways
Article Snippet: .. The following reagents were used in the present study: carboplatin, poly-D-lysine, U73122, KT5720, forskolin, and Hank’s balanced salt solution (Sigma-Aldrich, St Louis, MO, USA); fetal bovine serum (Gibco, Carlsbad, CA, USA); penicillin/streptomycin and AITC (Nacalai Tesque, Kyoto, Japan); blasticidin S and zeocin (Invitrogen, Carlsbad, CA, USA); acetone, 0.25% trypsin-EDTA, Dulbecco’s modified Eagle’s medium and tetracycline (Fujifilm Wako Pure Chemical, Osaka, Japan); fura-2 acetoxymethyl ester (Dojindo Laboratories, Kumamoto, Japan); AKAP I (Tocris Bioscience, Bristol, UK). ..

Virus:

Article Title: O 2 -Dependent Protein Internalization Underlies Astrocytic Sensing of Acute Hypoxia by Restricting Multimodal TRPA1 Channel Responses.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-TRPA1 [82] N/A Mouse monoclonal anti-GFAP Sigma-Aldrich Cat# G3893, RRID:AB_477010 Goat polyclonal anti-ChAT Thermo Fisher Scientific Cat# PA1-4710, RRID:AB_1089881 Rat polyclonal anti-CD31 BD pharmingen Cat#550274; RRID:AB_393571 Rabbit polyclonal anti-GFP Clontech Cat# 632592, RRID:AB_2336883 Rabbit polyclonal anti-TRPA1(Hydroxylated 394) [31] N/A Mouse monoclonal anti-a-tubulin Cell Signaling Cat# T6074, RRID:AB_477582 Mouse monoclonal anti-EEA1 Medical & Biological Laboratories Co., Ltd Cat# M176-3; RRID:AB_10693915 Rat monoclonal anti-LAMP1 Abcam Cat# ab25245; RRID:AB_449893 Mouse monoclonal anti-Synaptophysin Sigma-Aldrich Cat# S5768; RRID:AB_477523 anti-rabbit IgG-Cy3 Rockland Cat# 611-104-122, RRID:AB_218568 anti-mouse IgG-Alexa Fluor 488 Thermo Fisher Scientific Cat# A11001; RRID:AB_2534069 anti-rabbit IgG-Alexa Fluor 488 Thermo Fisher Scientific Cat# A11008; RRID:AB_143165 anti-mouse IgG-Cy3 Rockland Cat# 610-104-121; RRID:AB_218459 Rabbit anti-Flag M2 Stratagene Cat# 200470-21 Bacterial and Virus Strains Yeast: AH109 Clontech N/A Biological Samples Mouse Brain, whole Marathon-Ready cDNA BD Biosciences N/A Chemicals, Peptides, and Recombinant Proteins Fura-2 AM Dojindo Cat# F016 MG-132 Calbiochem Cat# 474790 Heclin Sigma Cat# SML1396 DMOG Sigma Cat# D3695 Quinacrine Sigma Cat# Q3251 SNAP Dojindo Cat#N415 H2O2 Wako Cat#080-01186 Phenyl disulfide Wako Cat#D487630 15-deoxy-D12,14-prostaglandin J2 Cayman Chemical Cat#18570 2-APB Calbiochem Cat#100065 AITC Nacalai Cat# 01415-92 BAPTA-AM Dojindo Cat# B035 Sulfo-NHS-SS-Biotin Thermo Scientific Cat# 21331 AP-18 Sigma Cat# A7232 Dynasore Sigma Cat# D7693 HC-030031 Sigma Cat# H4415 VH298 Abcam Cat# ab230370 A-967079 Wako Cat# 010-26981 Pitstop 1-25 Abcam Cat# ab144651 Fluoroshield Mounting Medium Funakoshi Cat# AR-6500-01 Tetanus toxin from Clostridium tetani Sigma-Aldrich Cat# T3194 ISOGEN Nippon Gene Cat# 311-02501 pyridoxalphosphate-6-azophenyl-20,4’-disulfonic acid Sigma-Aldrich Cat# P178 Mitoquinone mesylate MedChemExpress Cat# HY-100116A BCECF-AM Dojindo Cat# 344-05431 (Continued on next page) e1 Current Biology 30, 1–19.e1–e7, September 7, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-(p-amylcinnamoyl) anthranilic acid Sigma-Aldrich Cat# A8486 Brefeldin A Sigma Cat# B6542 Filipin Sigma Cat# F4767 Nifedipine Wako Cat# 145-05781 Critical Commercial Assays ENLITEN ATP Assay System Promega Cat# FF2000 Signal-SeekerTM Ubiquitination Detection Kit Cytoskeleton Inc. Cat# BK161 Experimental Models: Cell Lines Human: HEK293 cells Boehringer Ingelheim N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J Japan SLC,Inc.

Recombinant:

Activation Assay:

Article Title: Repetitive pulsed-wave ultrasound stimulation suppresses neural activity by modulating ambient GABA levels via effects on astrocytes
Article Snippet: For inhibition of inhibitory synaptic transmission, we used the GABA A receptor antagonists bicuculline ( ) (10 μM; Enzo Life Sciences # BML-EA149) and SR95531 ( ) (500 nM, Gabazine; ABCAM # ab120042). .. For activation of TRPA1, we used AITC ( ) (100 μM in DMSO, allyl isothiocyanate; Nacalai # 01415–92). .. For inhibition of inhibitory synaptic transmission, we used the GABA A receptor antagonists bicuculline ( ) (10 μM; Enzo Life Sciences # BML-EA149) and SR95531 ( ) (500 nM, Gabazine; ABCAM # ab120042).

Mouse Assay:

Article Title: A Cellulose-Rich Diet Disrupts Gut Homeostasis and Leads to Anxiety through the Gut-Brain Axis
Article Snippet: .. 58 Mice were habituated in the 15 cm × 10 cm × 8 cm observation cage for 20 min. AITC (2% vol vol –1 in rape seed oil; Nacalai Tesque Inc., Kyoto, Japan) was administered in 0.05 mL intrarectally, and their behavior was then observed for 15 min. Pain-related behaviors were determined as follows: licking of the abdomen, stretching the abdomen, squashing of the lower abdomen against the floor, and immobility. ..

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