Journal: International Journal of Molecular Sciences

Article Title: TRPA1-Activated Peptides from Saiga Antelope Horn: Screening, Interaction Mechanism, and Bioactivity

doi: 10.3390/ijms26052119

Figure Lengend Snippet: THP and AITC treatments of RIN-14B cells induce Ca 2+ influx and 5-HT release and are positively modulated by the TRPA1 channel. ( A , B ) Fluorescence and its relative quantification for Ca 2+ concentration upon THP and AITC treatments ( n = 3). The release of 5-HT from RIN-14B cells with RCWPDCR ( C ), FGFDGDF ( D ), WFCEGSF (200 μM) ( E ), and AITC, a TRPA1 agonist (30 μM), along with the inhibition of the TRPA1 channel with HC-030031 (30 μM) ( n = 3). Scale bar: 25 μm. Quantification data represented as mean ± SD. One-way ANOVA was applied for statistical analysis. * p < 0.05 vs. control; ** p < 0.01 vs. control; ## p < 0.01 vs. 200 μM THPs.

Article Snippet: Antelope horn powder (Cat no. 210403) was legally purchased from the Chengdu Minjiangyuan company (Chengdu, China); artificial gastric fluid (Cat no. SL6600) and artificial small intestine fluid (Cat no. SL6610A) were purchased from coolaber (Beijing, China); recombinant rat transient receptor potential cation channel subfamily A member 1 (TRPA1) and partial (Cat no. CSB-EP764869RA) were obtained from Cusabio (Wuhan, China); allyl isothiocyanate, a TRPA1 agonist (Cat no. 57-06-7), was purchased from Aladdin (Shanghai, China); HC-030031 (Cat no. HY-15064) was obtained from MedchemExpress (Princeton, NJ, USA); Dulbecco’s modified Eagle medium (Cat no. 8123621) was obtained from Gibco Inc. (Saranac, NY, USA); fetal bovine serum (Catalog No. FSP500) was obtained from Excell (Suzhou, China); a penicillin–streptomycin–amphotericin mixture (Catalog No. E607064-0100) was from Sangon Biotech (Shanghai, China); and Cell Counting Kit-8 (CCK-8) (Catalog No. BS350E) was purchased from Biosharp (Hefei, China); Fluo-4 AM eater (Catalog No. F3013S) was from UElandy (Suzhou, China); A cell total RNA isolation kit (RE-03111) and RT EasyTM II (RT-01022) were obtain from FOREGENE Biotech (Chengdu, China).

Techniques: Fluorescence, Quantitative Proteomics, Concentration Assay, Inhibition, Control

Journal: International Journal of Molecular Sciences

Article Title: TRPA1-Activated Peptides from Saiga Antelope Horn: Screening, Interaction Mechanism, and Bioactivity

doi: 10.3390/ijms26052119

Figure Lengend Snippet: The RT-qPCR validation in the examination of the effects of the TRPA1 agonist AITC (30 μM), the TRPA1 inhibitor HC-030031 (30 μM), THPs (200 μM), and THPs (200 μM) + HC-030031 (30 μM) on the levels of the genes related to the 5-HT synthesis pathway, TPH1, SERT, and Ddc ( n = 3). ( A ) RCWPDCR regulated the expression of the TPH1, Ddc, and SERT genes related to the 5-HT synthesis pathway. ( B ) FGFDGDF regulated the expression of the TPH1, Ddc and SERT genes related to the 5-HT synthesis pathway. ( C ) WFCEGSF regulated the expression of the TPH1, Ddc, and SERT genes related to the 5-HT synthesis pathway. * p < 0.05 vs. control; ** p < 0.01 vs. control; # p < 0.05 vs. 200 μM THPs; ## p < 0.01 vs. 200 μM THPs.

Article Snippet: Antelope horn powder (Cat no. 210403) was legally purchased from the Chengdu Minjiangyuan company (Chengdu, China); artificial gastric fluid (Cat no. SL6600) and artificial small intestine fluid (Cat no. SL6610A) were purchased from coolaber (Beijing, China); recombinant rat transient receptor potential cation channel subfamily A member 1 (TRPA1) and partial (Cat no. CSB-EP764869RA) were obtained from Cusabio (Wuhan, China); allyl isothiocyanate, a TRPA1 agonist (Cat no. 57-06-7), was purchased from Aladdin (Shanghai, China); HC-030031 (Cat no. HY-15064) was obtained from MedchemExpress (Princeton, NJ, USA); Dulbecco’s modified Eagle medium (Cat no. 8123621) was obtained from Gibco Inc. (Saranac, NY, USA); fetal bovine serum (Catalog No. FSP500) was obtained from Excell (Suzhou, China); a penicillin–streptomycin–amphotericin mixture (Catalog No. E607064-0100) was from Sangon Biotech (Shanghai, China); and Cell Counting Kit-8 (CCK-8) (Catalog No. BS350E) was purchased from Biosharp (Hefei, China); Fluo-4 AM eater (Catalog No. F3013S) was from UElandy (Suzhou, China); A cell total RNA isolation kit (RE-03111) and RT EasyTM II (RT-01022) were obtain from FOREGENE Biotech (Chengdu, China).

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: TRPA1-Activated Peptides from Saiga Antelope Horn: Screening, Interaction Mechanism, and Bioactivity

doi: 10.3390/ijms26052119

Figure Lengend Snippet: The Western blot validation in the examination of the effects of the TRPA1 agonist AITC (30 μM), the TRPA1 inhibitor HC-030031 (30 μM), THPs (200 μM), and THPs (200 μM) + HC-030031 (30 μM) on the levels of the genes related to the 5-HT synthesis pathway, TPH1, SERT, and Ddc ( n = 3). ( A , D ) RCWPDCR: representative bands of a Western blot for TPH1, SERT, and Ddc and their relative protein expression. ( B , E ) FGFDGDF: representative bands of a Western blot for TPH1, SERT, and Ddc and their relative protein expression. ( C , F ) WFCEGSF: representative bands of a Western blot for TPH1, SERT, and Ddc and their relative protein expression. * p < 0.05 vs. control; ** p < 0.01 vs. control; # p < 0.05 vs. 200 μM THPs; ## p < 0.01 vs. 200 μM THPs.

Article Snippet: Antelope horn powder (Cat no. 210403) was legally purchased from the Chengdu Minjiangyuan company (Chengdu, China); artificial gastric fluid (Cat no. SL6600) and artificial small intestine fluid (Cat no. SL6610A) were purchased from coolaber (Beijing, China); recombinant rat transient receptor potential cation channel subfamily A member 1 (TRPA1) and partial (Cat no. CSB-EP764869RA) were obtained from Cusabio (Wuhan, China); allyl isothiocyanate, a TRPA1 agonist (Cat no. 57-06-7), was purchased from Aladdin (Shanghai, China); HC-030031 (Cat no. HY-15064) was obtained from MedchemExpress (Princeton, NJ, USA); Dulbecco’s modified Eagle medium (Cat no. 8123621) was obtained from Gibco Inc. (Saranac, NY, USA); fetal bovine serum (Catalog No. FSP500) was obtained from Excell (Suzhou, China); a penicillin–streptomycin–amphotericin mixture (Catalog No. E607064-0100) was from Sangon Biotech (Shanghai, China); and Cell Counting Kit-8 (CCK-8) (Catalog No. BS350E) was purchased from Biosharp (Hefei, China); Fluo-4 AM eater (Catalog No. F3013S) was from UElandy (Suzhou, China); A cell total RNA isolation kit (RE-03111) and RT EasyTM II (RT-01022) were obtain from FOREGENE Biotech (Chengdu, China).

Techniques: Western Blot, Biomarker Discovery, Expressing, Control

Journal: Frontiers in Plant Science

Article Title: Weed suppression and antioxidant activity of Astragalus sinicus L. decomposition leachates

doi: 10.3389/fpls.2022.1013443

Figure Lengend Snippet: The concentration of goosegrass-suppressed active substances in different decay duration decomposition leachates.

Article Snippet: Allyl isothiocyanate was obtained from ChromaDex ® (J&K Scientific, China), with a purity of 99%.

Techniques: Concentration Assay

Journal: Frontiers in Plant Science

Article Title: Weed suppression and antioxidant activity of Astragalus sinicus L. decomposition leachates

doi: 10.3389/fpls.2022.1013443

Figure Lengend Snippet: Effect of different identified substances on germination and growth of goosegrass. Different letters above each group of the bar chart denoted significantly different among different concentration treatments of identified substance at P < 0.05 by LSD test, respectively. Bars represent 10, 3, and 5 repetitions ± SD in plant height, fresh weight, and germination-related parameters, respectively. (A) 4-Ethylphenol. (B) Allyl isothiocyanate. (C) N-acrylimorpholine. (D) 2-Hydroxyethyl acrylate. (E) Mixture of (A-D) .

Article Snippet: Allyl isothiocyanate was obtained from ChromaDex ® (J&K Scientific, China), with a purity of 99%.

Techniques: Concentration Assay

Journal: Frontiers in Plant Science

Article Title: Weed suppression and antioxidant activity of Astragalus sinicus L. decomposition leachates

doi: 10.3389/fpls.2022.1013443

Figure Lengend Snippet: RI accumulation value of different identified substances on germination and growth of goosegrass. (A) 4-Ethylphenol. (B) Allyl isothiocyanate. (C) N-acrylimorpholine. (D) 2-hydroxyethyl acrylate. (E) Mixture of (A) to (D) .

Article Snippet: Allyl isothiocyanate was obtained from ChromaDex ® (J&K Scientific, China), with a purity of 99%.

Techniques:

Journal: Langenbeck's Archives of Surgery

Article Title: Indocyanine green and height of anastomosis in colorectal surgery– a network meta-analysis

doi: 10.1007/s00423-025-03765-x

Figure Lengend Snippet: Network plot for direct comparisons of anastomotic leak in studies of High anterior resection (HAR) and Low anterior resection (LAR) and use of indocyanine green (ICG)

Article Snippet: Hasegawa et al. [ ] , 2020 , 2007–2017 (Japan) , Retrospective cohort , 852 , Low anterior resection or intersphincteric resections for rectal cancer , ICG 5 mg before , No ICG in matched controls during the same time period , AL , Lap (serosa) , Cancer.

Techniques:

Journal: Langenbeck's Archives of Surgery

Article Title: Indocyanine green and height of anastomosis in colorectal surgery– a network meta-analysis

doi: 10.1007/s00423-025-03765-x

Figure Lengend Snippet: Forest plot comparing interventions in relation to rates of anastomotic leak in studies of High anterior resection (HAR) and Low anterior resection (LAR) and use of indocyanine green (ICG)

Article Snippet: Hasegawa et al. [ ] , 2020 , 2007–2017 (Japan) , Retrospective cohort , 852 , Low anterior resection or intersphincteric resections for rectal cancer , ICG 5 mg before , No ICG in matched controls during the same time period , AL , Lap (serosa) , Cancer.

Techniques:

Journal: Langenbeck's Archives of Surgery

Article Title: Indocyanine green and height of anastomosis in colorectal surgery– a network meta-analysis

doi: 10.1007/s00423-025-03765-x

Figure Lengend Snippet: Pairwise comparisons of odds ratios of anastomotic leak, in network meta-analysis of studies of High anterior resection (HAR) and Low anterior resection (LAR) and with and without use of indocyanine green (ICG)

Article Snippet: Hasegawa et al. [ ] , 2020 , 2007–2017 (Japan) , Retrospective cohort , 852 , Low anterior resection or intersphincteric resections for rectal cancer , ICG 5 mg before , No ICG in matched controls during the same time period , AL , Lap (serosa) , Cancer.

Techniques:

Journal: Langenbeck's Archives of Surgery

Article Title: Indocyanine green and height of anastomosis in colorectal surgery– a network meta-analysis

doi: 10.1007/s00423-025-03765-x

Figure Lengend Snippet: Rankogram with probability for ranking first to fourth (least likely to be associated with anastomotic leak to most likely respectively). The probability ranges from 1.0 (most likely to fit in assigned ranking) to 0 (least likely). Studies are based on low (LAR) vs. high anterior resection (HAR) with and without indocyanine green (ICG). Intervention on left of graph, with higher surface under the cumulative ranking curve (SUCRA) is best

Article Snippet: Hasegawa et al. [ ] , 2020 , 2007–2017 (Japan) , Retrospective cohort , 852 , Low anterior resection or intersphincteric resections for rectal cancer , ICG 5 mg before , No ICG in matched controls during the same time period , AL , Lap (serosa) , Cancer.

Techniques: