anti adamts4 (Proteintech)

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Proteintech anti adamts4
Anti Adamts4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adamts4/ADAMTS4+Fusion+Protein/pmc12874112-153-32-40
Average 94 stars, based on 11 article reviews

anti adamts4 - by Bioz Stars, 2026-09

94/100 stars

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Immunohistochemical staining:

Article Title: Rabbits as preclinical animal models of cam morphology: proof of concept.
Article Snippet: .. For immunohistochemical (IHC) staining, primary antibodies for Runx2 (1:100, PA5-82787, Invitrogen, The Netherlands), ACAN (1:30, 13880-1-AP, Proteintech, China), Sox9 (1:200, PA5-81966, Invitrogen, The Netherlands), and ADAMTS4 (1:30, 11865-1-AP, Proteintech, China) were used with matching secondary antibodies (1:3000, BK-0201 and BK-0202, AimokeBio, China). .. A TUNEL detection kit (PF00006, Proteintech, China) was used per the manufacturer’s instructions for a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay of each section.

Article Title: Rabbits as preclinical animal models of cam morphology: proof of concept
Article Snippet: .. For immunohistochemical (IHC) staining, primary antibodies for Runx2 (1:100, PA5-82787, Invitrogen, The Netherlands), ACAN (1:30, 13880-1-AP, Proteintech, China), Sox9 (1:200, PA5-81966, Invitrogen, The Netherlands), and ADAMTS4 (1:30, 11865-1-AP, Proteintech, China) were used with matching secondary antibodies (1:3000, BK-0201 and BK-0202, AimokeBio, China). .. A TUNEL detection kit (PF00006, Proteintech, China) was used per the manufacturer’s instructions for a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay of each section.

Immunohistochemistry:

Staining:

Incubation:

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway
Article Snippet: .. Antigen retrieval was performed by incubating the sections in 1 mM EDTA antigen retrieval solution (pH = 9) in a water bath at 95 °C for 45 min. After rinsing with PBS, the sections were blocked with 5% BSA at room temperature for 1 h. The sections were then incubated with primary antibodies MMP13 (1:600, ProteinTech, China), ADAMTS4 (1:100, Affinity, USA), ADAMTS5 (1:100, Affinity, USA), and β-CATENIN (1:1000, ProteinTech, China) at 4 °C overnight. ..

Article Title: PIM3 enhances nucleus pulposus cell function and extracellular matrix integrity via kinase-dependent activation of the Akt/mTOR signaling axis in intervertebral disc degeneration.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway.
Article Snippet: .. Antigen retrieval was performed by incubating the sections in 1 mM EDTA antigen retrieval solution (pH = 9) in a water bath at 95 °C for 45 min. After rinsing with PBS, the sections were blocked with 5% BSA at room temperature for 1 h. The sections were then incubated with primary antibodies MMP13 (1:600, ProteinTech, China), ADAMTS4 (1:100, Affinity, USA), ADAMTS5 (1:100, Affinity, USA), and β-CATENIN (1:1000, ProteinTech, China) at 4 °C overnight. ..

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway
Article Snippet: .. After washing with TBST for 3 × 5 min, the membrane was incubated overnight at 4 °C with primary antibodies MMP13 (1:1000, ProteinTech, China), ADAMTS4 (1:800, Affinity, USA), ADAMTS5 (1:800, Affinity, USA), β-CATENIN (1:2000, ProteinTech, China), GSK 3β (1:1000, Cell Signaling Technology, USA), and p-GSK 3β-Ser9 (1:1000, Cell Signaling Technology, USA). .. After washing with TBST for 3 × 5 min, the membrane was incubated with a secondary antibody (1:1000) at room temperature for 1 h. Following washing with TBST for 6 × 5 min, the membrane was exposed using an ECL substrate (Millipore, Germany) to visualize the target proteins.

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway.
Article Snippet: .. After washing with TBST for 3 × 5 min, the membrane was incubated overnight at 4 °C with primary antibodies MMP13 (1:1000, ProteinTech, China), ADAMTS4 (1:800, Affinity, USA), ADAMTS5 (1:800, Affinity, USA), β-CATENIN (1:2000, ProteinTech, China), GSK 3β (1:1000, Cell Signaling Technology, USA), and p-GSK 3β-Ser9 (1:1000, Cell Signaling Technology, USA). .. After washing with TBST for 3 × 5 min, the membrane was incubated with a secondary antibody (1:1000) at room temperature for 1 h. Following washing with TBST for 6 × 5 min, the membrane was exposed using an ECL substrate (Millipore, Germany) to visualize the target proteins.

Blocking Assay:

Membrane:

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway
Article Snippet: .. After washing with TBST for 3 × 5 min, the membrane was incubated overnight at 4 °C with primary antibodies MMP13 (1:1000, ProteinTech, China), ADAMTS4 (1:800, Affinity, USA), ADAMTS5 (1:800, Affinity, USA), β-CATENIN (1:2000, ProteinTech, China), GSK 3β (1:1000, Cell Signaling Technology, USA), and p-GSK 3β-Ser9 (1:1000, Cell Signaling Technology, USA). .. After washing with TBST for 3 × 5 min, the membrane was incubated with a secondary antibody (1:1000) at room temperature for 1 h. Following washing with TBST for 6 × 5 min, the membrane was exposed using an ECL substrate (Millipore, Germany) to visualize the target proteins.

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway.
Article Snippet: .. After washing with TBST for 3 × 5 min, the membrane was incubated overnight at 4 °C with primary antibodies MMP13 (1:1000, ProteinTech, China), ADAMTS4 (1:800, Affinity, USA), ADAMTS5 (1:800, Affinity, USA), β-CATENIN (1:2000, ProteinTech, China), GSK 3β (1:1000, Cell Signaling Technology, USA), and p-GSK 3β-Ser9 (1:1000, Cell Signaling Technology, USA). .. After washing with TBST for 3 × 5 min, the membrane was incubated with a secondary antibody (1:1000) at room temperature for 1 h. Following washing with TBST for 6 × 5 min, the membrane was exposed using an ECL substrate (Millipore, Germany) to visualize the target proteins.

other:

Article Title: Targeted knockdown of Piezo1 in synovial macrophages attenuates osteoarthritis development
Article Snippet: ADAMTS4 , 82744-2-RR , Proteintech Group (Chicago, IL, USA) , 1:1000 , 1:200.

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