Journal: Scientific Reports

Article Title: Loss of ADAMTS4 reduces high fat diet-induced atherosclerosis and enhances plaque stability in ApoE −/− mice

doi: 10.1038/srep31130

Figure Lengend Snippet: Plaque characteristics in brachiocephalic artery (n = 5 in all groups, with 5 sections per mouse).

Article Snippet: Recombinant human ADAMTS4 (R&D Systems, USA) was used to plot the standard curve.

Techniques:

Journal: Scientific Reports

Article Title: Loss of ADAMTS4 reduces high fat diet-induced atherosclerosis and enhances plaque stability in ApoE −/− mice

doi: 10.1038/srep31130

Figure Lengend Snippet: ( a ) ADAMTS4 expression in the plaques of 12 and 18 weeks old ApoE −/− mice. ( b ) Quantification of the ADAMTS4 positive stained area expressed as percentage of total lesion area (n = 5 mice, with 5 sections from each brachiocephalic artery). ( c ) Plasma ADAMTS4 level in ApoE-null mice (n = 8). Values shown are mean ± SEM.

Article Snippet: Recombinant human ADAMTS4 (R&D Systems, USA) was used to plot the standard curve.

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: Loss of ADAMTS4 reduces high fat diet-induced atherosclerosis and enhances plaque stability in ApoE −/− mice

doi: 10.1038/srep31130

Figure Lengend Snippet: Co-localization of ADAMTS4 with CD68 ( a ) and SMA ( b ) in brachiocephalic aortic plaques of 18 weeks old ApoE −/− mice.

Article Snippet: Recombinant human ADAMTS4 (R&D Systems, USA) was used to plot the standard curve.

Techniques:

Journal: Experimental & Molecular Medicine

Article Title: Differentiation of adipose-derived stem cells into functional chondrocytes by a small molecule that induces Sox9

doi: 10.1038/s12276-020-0424-y

Figure Lengend Snippet: a Structure of ellipticine. b Concentration-dependent cell viability was measured by cell viability assays in ASCs. D DMSO-treated ASCs * p < 0.05, *** p < 0.001 compared to the control. n = 3. c The ability of ELPC to induce differentiation was confirmed using Alcian blue staining after ASCs were incubated with ELPC for 16 days. Scale bar: 3 mm. d A 3D pellet culture was performed using ASCs or ELPC-treated ASCs for 16 days. ** p < 0.01 compared to the control. n = 3. e The untreated ASC and ELPC-treated ASC pellets were stained using Alcian blue and H&E. The expression of Type II collagen, Aggrecan, and Type X collagen was detected by immunofluorescence. Nuclei were stained with DAPI (Blue). Scale bar: upper 400 μm and lower 50 μm. f The mRNA expression of the extracellular matrix degradation enzymes ADAMTS4, ADAMTS5, and MMP13. Normalized by GAPDH. * p < 0.05, *** p < 0.001 compared to the control. n = 3. g The concentrations of secreted ADAMTS4 and ADAMTS5 were measured by ELISAs. *** p < 0.001 compared to the control, # p < 0.001 compared to the 16-day control, $ p < 0.001 compared to the ELPC-treated ASCs. C and Cont control, DM chondrogenic differentiation induction medium, ELPC ELPC-treated ASCs. n = 3.

Article Snippet: The protein activities of ADAMTS4 and ADAMTS5, which are released as active protease forms in the cell culture supernatant, were measured using ADAMTS4 and ADAMTS5 ELISA kits (R&D Systems).

Techniques: Concentration Assay, Control, Staining, Incubation, Expressing, Immunofluorescence

Journal: International journal of oncology

Article Title: Expression of ADAMTS4 in Ewing's sarcoma.

doi: 10.3892/ijo_00000706

Figure Lengend Snippet: Figure 1. Effects of EWS-FLI1 suppression on ADAMTS4 expression. RNA from Ewing's sarcoma cells treated with either siEF1 or siGFP were subjected to RT-PCR experiment. ADAMTS4 mRNA expression was down- regulated after treatment with EWS-FLI1-specific siRNA. GAPDH (glyceral- dehyde 3-phosphate dehydrogenase) was used as an internal control.

Article Snippet: For ELISA, a monoclonal anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 (R&D Systems), a biotinylated goat anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 and an Avidin-HRP (eBioscience, San Diego, CA, USA) were used.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control

Journal: International journal of oncology

Article Title: Expression of ADAMTS4 in Ewing's sarcoma.

doi: 10.3892/ijo_00000706

Figure Lengend Snippet: Figure 2. Immunohistochemical analysis of ADAMTS4 protein in the tissue section of the patient with Ewing's sarcoma. (A) Hematoxylin and eosin staining, (B) rabbit IgG, (C) anti-ADAMTS4 antibody.

Article Snippet: For ELISA, a monoclonal anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 (R&D Systems), a biotinylated goat anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 and an Avidin-HRP (eBioscience, San Diego, CA, USA) were used.

Techniques: Immunohistochemical staining, Staining

Journal: International journal of oncology

Article Title: Expression of ADAMTS4 in Ewing's sarcoma.

doi: 10.3892/ijo_00000706

Figure Lengend Snippet: Figure 3. Immunofluorescence staining of ADAMTS4 protein in Ewing's sarcoma cell lines (SJES-2 and SJES-5), osteosarcoma cell lines (MG63 and SaOS2) and NIH3T3 cells expressing ADAMTS4. (A) NIH3T3, (B) ADAMTS4/NIH3T3, (C) SJES-5, (D) SJES-2, (E) MG63, (F) SaOS2.

Article Snippet: For ELISA, a monoclonal anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 (R&D Systems), a biotinylated goat anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 and an Avidin-HRP (eBioscience, San Diego, CA, USA) were used.

Techniques: Immunofluorescence, Staining, Expressing

Journal: International journal of oncology

Article Title: Expression of ADAMTS4 in Ewing's sarcoma.

doi: 10.3892/ijo_00000706

Figure Lengend Snippet: Figure 4. Detection of secreted ADAMTS4 protein. The cell lysates or culture supernatants were immunoprecipitated with the anti-ADAMTS4 antibody, resolved by SDS-PAGE, blotted and probed with the anti-ADAMTS4 antibody. Molecular size markers are shown on the left. Arrows indicate the ADAMTS4 proteins. (A), cell lysates; (B), supernatants.

Article Snippet: For ELISA, a monoclonal anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 (R&D Systems), a biotinylated goat anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 and an Avidin-HRP (eBioscience, San Diego, CA, USA) were used.

Techniques: Immunoprecipitation, SDS Page

Journal: International journal of oncology

Article Title: Expression of ADAMTS4 in Ewing's sarcoma.

doi: 10.3892/ijo_00000706

Figure Lengend Snippet: Figure 5. RT-PDR analysis of ADAMTS4 expression in the patient samples. GAPDH expression was used as an internal control.

Article Snippet: For ELISA, a monoclonal anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 (R&D Systems), a biotinylated goat anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 and an Avidin-HRP (eBioscience, San Diego, CA, USA) were used.

Techniques: Expressing, Control

Journal: International journal of oncology

Article Title: Expression of ADAMTS4 in Ewing's sarcoma.

doi: 10.3892/ijo_00000706

Figure Lengend Snippet: Figure 6. ELISA of ADAMTS4 protein in the patient sera and the supernatants of the cell lines. The error bars represent 1 standard deviation.

Article Snippet: For ELISA, a monoclonal anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 (R&D Systems), a biotinylated goat anti-ADAMTS4 antibody raised against amino acids 213-685 of the recombinant human ADAMTS4 and an Avidin-HRP (eBioscience, San Diego, CA, USA) were used.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation