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gapdh acris antibody  (OriGene)


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    Structured Review

    OriGene gapdh acris antibody
    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using <t>GaPDH</t> as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
    Gapdh Acris Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acris/GAPDH+Rabbit+Polyclonal+Antibody/bio_rxiv__2025__11__11__687795-146-9-10
    Average 94 stars, based on 20 article reviews
    gapdh acris antibody - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "JPT2/HN1L functions as an NAADP-binding protein in a cell type-specific manner"

    Article Title: JPT2/HN1L functions as an NAADP-binding protein in a cell type-specific manner

    Journal: bioRxiv

    doi: 10.1101/2025.11.11.687795

    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using GaPDH as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
    Figure Legend Snippet: (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using GaPDH as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.

    Techniques Used: Cloning, Knock-Out, CRISPR, Western Blot

    Related Articles

    Immunohistochemistry:

    Article Title: YAP drives cutaneous squamous cell carcinoma formation and progression
    Article Snippet: Antibody (Rabbit monoclonal) , anti-Keratin-5 , Abcam , (Abcam Cat# ab52635, RRID: AB_869890 ) , 1/500 IHC. .. Antibody (Rabbit polyclonal) , anti-beta- galactosidase , Acris , (Acris Antibodies GmbH Cat# R1064P, RRID: AB_973264 ) , 1/5000 IHC. .. Antibody (Rabbit polyclonal) , anti-E-Cadherin , Santa Cruz , (Santa Cruz Biotechnology Cat# sc-7870, RRID: AB_2076666 ) , 1/75 IHC.

    Binding Assay:

    Article Title: Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
    Article Snippet: .. The nonspecific binding sites were blocked by incubation with 5% fat-free dry milk in a TBS-T buffer at room temperature (RT) for 1.5 h. The nitrocellulose membranes were incubated overnight (at 4°C) with primary polyclonal antibodies including: rabbit anti-human Y1Rs antibody (diluted 1:500, cat. no. AP01221PU-N) and rabbit anti-human Y2Rs antibody (diluted 1:1000, cat. no. TA314282) both from Acris an OriGene Company. .. After being washed in TBS-T buffer, the nitrocellulose membranes were incubated with biotinylated goat anti-rabbit IgG (diluted 1:3000, cat. no. PK-6101, Vectastain Elite ABC-HRP Kit, Vector Labs, Burlingame, CA, USA) for 1 h, at RT.

    Incubation:

    Article Title: Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
    Article Snippet: .. The nonspecific binding sites were blocked by incubation with 5% fat-free dry milk in a TBS-T buffer at room temperature (RT) for 1.5 h. The nitrocellulose membranes were incubated overnight (at 4°C) with primary polyclonal antibodies including: rabbit anti-human Y1Rs antibody (diluted 1:500, cat. no. AP01221PU-N) and rabbit anti-human Y2Rs antibody (diluted 1:1000, cat. no. TA314282) both from Acris an OriGene Company. .. After being washed in TBS-T buffer, the nitrocellulose membranes were incubated with biotinylated goat anti-rabbit IgG (diluted 1:3000, cat. no. PK-6101, Vectastain Elite ABC-HRP Kit, Vector Labs, Burlingame, CA, USA) for 1 h, at RT.

    Staining:

    Article Title: PDGF-A suppresses contact inhibition during directional collective cell migration.
    Article Snippet: .. F-Actin was stained in specimens fixed in 4% paraformaldehyde (20 min, 0.01% Triton-X-100 added after 5 minutes) with Alexa Fluor 488 phalloidin or rhodamine phalloidin (Invitrogene) at 1:100 in PBS/BSA for 20 min. Rabbit antiserum against Xenopus laevis plasma FN (Winklbauer, 1998) (1:1000), mouse monoclonal antibody 8C8 against Xenopus laevis integrin1 (DSHB, Hausen, P. and Gawantka, V.) (1: 6), polyclonal rabbit antibody against syndecan-4 (SDC4) (1:200, TA 314520, Acris, OriGene), and polyclonal rabbit antibody against ephrin-B1, (1:200, A-20, sc – 1011, Santa Cruz Biotechnology; Lee et al. 2009), and PDGFRα (1:2000, 3164, Cell Signaling Technology; Bahm et al. 2017), were used. .. Secondary antibodies were Cy3-goat-antirabbit IgG, FITC-goat-anti-rabbit IgG, and Cy3-goat-anti-mouse IgG (Jackson Immuno Research Lab, Inc.).

    Clinical Proteomics:

    Article Title: PDGF-A suppresses contact inhibition during directional collective cell migration.
    Article Snippet: .. F-Actin was stained in specimens fixed in 4% paraformaldehyde (20 min, 0.01% Triton-X-100 added after 5 minutes) with Alexa Fluor 488 phalloidin or rhodamine phalloidin (Invitrogene) at 1:100 in PBS/BSA for 20 min. Rabbit antiserum against Xenopus laevis plasma FN (Winklbauer, 1998) (1:1000), mouse monoclonal antibody 8C8 against Xenopus laevis integrin1 (DSHB, Hausen, P. and Gawantka, V.) (1: 6), polyclonal rabbit antibody against syndecan-4 (SDC4) (1:200, TA 314520, Acris, OriGene), and polyclonal rabbit antibody against ephrin-B1, (1:200, A-20, sc – 1011, Santa Cruz Biotechnology; Lee et al. 2009), and PDGFRα (1:2000, 3164, Cell Signaling Technology; Bahm et al. 2017), were used. .. Secondary antibodies were Cy3-goat-antirabbit IgG, FITC-goat-anti-rabbit IgG, and Cy3-goat-anti-mouse IgG (Jackson Immuno Research Lab, Inc.).

    other:

    Article Title: Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia
    Article Snippet: Mouse IgG1 anti-CC10 , Acris, Origene , #AM26360PU-N.



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    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using <t>GaPDH</t> as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
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    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using <t>GaPDH</t> as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
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    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using <t>GaPDH</t> as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
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    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using <t>GaPDH</t> as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
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    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using <t>GaPDH</t> as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
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    Image Search Results


    (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using GaPDH as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.

    Journal: bioRxiv

    Article Title: JPT2/HN1L functions as an NAADP-binding protein in a cell type-specific manner

    doi: 10.1101/2025.11.11.687795

    Figure Lengend Snippet: (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using GaPDH as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.

    Article Snippet: The Blot was incubated with an JPT2 (Sigma) or GAPDH (Acris) antibody at 4 °C overnight.

    Techniques: Cloning, Knock-Out, CRISPR, Western Blot