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Journal: bioRxiv
Article Title: JPT2/HN1L functions as an NAADP-binding protein in a cell type-specific manner
doi: 10.1101/2025.11.11.687795
Figure Lengend Snippet: (A) cloning strategy of generation of Jpt2/Hn1l knockout mouse using CrisPR-Cas9. Using two gRNAs flanking exon 3 to delete exon 3 and cause a premature stop codon (B) PCR to show genetic deletion of exon 3 by Q5-polymerase PCR usingprimers flanking exon 3. Left reaction (Primer 1 (Jpt2-Wf/KO-fw) and 2 (Jpt2-Wf-rev2)) results in WT band of 410bp) and right reaction (primers 1 (Jpt2-Wf/Ko-fw) and3 (Jpt2-KO-rev)) shows a knockout band at 299bp and a faint WTband at 900bp. (C) Furthermore, deletion of JPT2 protein in spleens was confirmed by Western Blot using GaPDH as house keeping protein. Each datapoint represents protein from a single mouse. Statistical analysis was performed according to unpaired Student’s t-test.
Article Snippet: The Blot was incubated with an JPT2 (Sigma) or
Techniques: Cloning, Knock-Out, CRISPR, Western Blot